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Glycerol-based liquid formulation of the epiphytic yeast Hanseniaspora guilliermondii isolate YBB3 with multiple modes of action controls postharvest Aspergillus rot in grapes

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Abstract

Postharvest loss is a major constraint in the production of grapes, both in terms of quality and quantity. In this study, 33 yeasts were isolated from the fructosphere of grapes and tested for their antifungal activity against Aspergillus spp., which infects grapes during the postharvest stage. The results revealed that the yeast isolate YBB3 exhibited maximum inhibitory effect against Aspergillus spp. in dual plate assay (57% over control). Molecular characterization of YBB3 showed 99.31% identity with Hanseniaspora guilliermondii. Wound site colonization of H. guilliermondii in grape cv. Thompson Seedless showed 80% inhibition of Aspergillus spp. over the control in vitro. YBB3 produced volatile, non-volatile and thermostable compounds, which inhibited the mycelial growth of Aspergillus spp. In addition, YBB3 produced killer toxins, which were identified by the presence of blue colour-stained cells. Moreover, glycerol-based liquid bioformulations of H. guilliermondii (YBB3) showed maximum inhibitory effect against Aspergillus spp. (96%) over the control on inoculated wounded grapes berries. Thus, the yeast isolate H. guilliermondii identified and characterized in this study is worthy of further studies for the sustainable management of Aspergillus rot in grapes.

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Acknowledgements

The authors acknowledge the Department of Plant Pathology, Agricultural College and Research Institute, Madurai, Tamil Nadu, India for providing necessary facilities to carry out the research work.

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Authors and Affiliations

  1. Department of Plant Pathology, Agricultural College and Research Institute, Madurai, Tamil Nadu, India

    Murugan Nandhini & Sankarasubramanian Harish

  2. Agricultural College and Research Institute, Killikulam, Tamil Nadu, India

    K. Eraivan Arutkani Aiyanathan

  3. Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

    Sankarasubramanian Harish & Dhakshinamoorthy Durgadevi

  4. Department of Horticulture, Agricultural College and Research Institute, Madurai, Tamil Nadu, India

    Andithevar Beaulah

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  1. Murugan Nandhini
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Correspondence to Sankarasubramanian Harish.

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Nandhini, M., Harish, S., Aiyanathan, K.E.A. et al. Glycerol-based liquid formulation of the epiphytic yeast Hanseniaspora guilliermondii isolate YBB3 with multiple modes of action controls postharvest Aspergillus rot in grapes. J Plant Pathol 103, 1253–1264 (2021). https://doi.org/10.1007/s42161-021-00909-y

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