Abstract
Wheat dwarf virus (WDV) causes one of the most serious cereal diseases in many countries. In spring 2020, we observed wheat plants with symptoms of severe dwarfism, no heading/reduced heading, leaf streaking and yellowing in five regions located in western, central and northern parts of Ukraine. The aim of the study was to identify the virus causing mentioned symptoms, investigate its impact on wheat yield and perform phylogenetic analysis of the detected viral isolates. Using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and polymerase chain reaction, WDV was confirmed in 14 varieties of winter and spring wheat. CP gene regions of two WDV isolates were sequenced: Khm-K-Ukr, MW176036 (from winter wheat var. Kolonia, Khmelnytskyi region) and MIP-12-Ukr, MW176037 (winter wheat D-12-1, Kyiv region). Phylogenetic analysis showed that Khm-K-Ukr and MIP-12-Ukr belong to the clade E. These isolates have the highest level of sequence identity with the barley isolate WDV-Lip from Poland (KY781940), the Ukrainian wheat isolate WDV-Uk-Miron (FN806784) and the isolate from Estonia (MK193742) and clustered on the same branch indicating their possible common origin. The isolate MIP-12-Ukr has 1 unique aa substitution and Khm-K-Ukr has 4 aa mutations in the studied CP gene region in comparison with other 52 WDVs from GenBank. It was found that WDV significantly reduced the number of seeds per spike 2.6–3.4 -fold, the weight of seeds per spike (3.8–4.2-fold), while weight of 1000 grains was also decreased (by 2.6–3.5 times). These results indicate that WDV is a big threat to wheat crops in Ukraine.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Availability of data and materials
All data generated or analyzed during this study are included in this published article (and its additional files). The Khm-K-Ukr and MIP-12-Ukr sequences have been deposited in the NCBI GenBank [accession numbers MW176036 and MW176037] and have not been released to the public database yet but are available from the corresponding author on reasonable request.
Code availability
Not applicable.
References
Achon MA, Serrano L, Ratti C, Rubies-Autonell C (2006) First detection of Wheat dwarf virus in barley in Spain associated with an outbreak of Barley Yellow Dwarf. Plant Dis 90:970. https://doi.org/10.1094/PD-90-0970A
Behjatnia S, Afsharifar A, Tahan V, Motlagh MA, Gandomani OE, Niazi A, Izadpanah K (2011) Widespread occurrence and molecular characterization of Wheat dwarf virus in Iran. Australas Plant Pathol 40:12–19. https://doi.org/10.1007/s13313-010-0008-8
Bendahmane M, Schalk HJ, Gronenborn B (1995) Identification and characterization of wheat dwarf virus from France using a rapid method for geminivirus DNA preparation. Phytopathology 85(11):1449–1455. https://doi.org/10.1094/Phyto-85-1449
Bisztray G, Gáborjányi R, Vacke J (1989) Isolation and characterization of wheat dwarf virus found for the first time in Hungary. J Plant Dis Prot 96:449–454
Crowther JR (1995) ELISA. Theory and practice. Humana Press, New York
Ekzayez AM, Kumari SG, Ismail I (2011) First report of Wheat dwarf virus and its vector (Psammotettix provincialis) affecting wheat and barley crops in Syria. Plant Dis 95:76. https://doi.org/10.1094/PDIS-09-10-0628
Fohrer F, Lebrun I, Lapierre H (1992) Acquisitions recente sur le virus du nanisme du ble. Phytoma 443:18–20 (in French)
Ghodoum Parizipour MH, Ramazani L, Pakdaman Sardrood B (2018) Temperature affected transmission, symptom development and accumulation of Wheat dwarf virus. Plant Prot Sci 54:222–233. https://doi.org/10.17221/147/2017-PPS
Guliaeva II, Shevchenko OV, Snigur GO, Bisov AS, Milkus BN (2011) Spread of wheat dwarf virus (WDV) on the South of Ukraine. Microbiol Biotechnol 3:71–77 (in Ukrainian)
Gutierrez C (2002) Strategies for geminivirus DNA replication and cell cycle interference. Physiol Mol Plant Path 60:219–230. https://doi.org/10.1006/pmpp.2002.0401
Huelsenbeck JP, Rannala B (1997) Maximum likelihood estimation of phylogeny using stratigraphic data. Paleobiology 23(2):174–180. https://doi.org/10.1017/S0094837300016778
Huth W (2000) Viruses of Graminae in Germany—a short overview. J Plant Dis Prot 107:406–414
Jeżewska M (2011) First report of Wheat dwarf virus occurring in Poland. Phytopathol Pol 21:93–100
Jilaveanu A, Vacke J (1995) Isolation and identification of wheat dwarf virus (WDV) in Romania. Probleme De Protectia Plantelor 23:51–62
Jones RAC (2021) Global plant virus disease pandemics and epidemics. Plants 10:233. https://doi.org/10.3390/plants10020233
Kapooria RG, Ndunguru J (2004) Occurrence of viruses in irrigated wheat in Zambia. EPPO Bull 34:413–419. https://doi.org/10.1111/j.1365-2338.2004.00771.x
Koklu G, Ramsell N, Kvarnheden A (2007) The complete genome sequence for a Turkish isolate of Wheat dwarf virus (WDV) from barley confirms the presence of two distinct WDV strains. Virus Genes 34(3):359–366. https://doi.org/10.1007/s11262-006-0029-0
Korber B (2002) HIV sequence sigmatires and similarities. In: Rodrigo AG, Learn GH (eds) Computational and evolutionary analysis of HIV molecular sequences. Springer, Boston, pp 55–72. https://doi.org/10.1007/0-306-46900-6_4
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874. https://doi.org/10.1093/molbev/msw054
Kundu JK, Gadiou S, Cervena G (2009) Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic. Virus Genes 38(3):468–474. https://doi.org/10.1007/s11262-009-0352-3
Kvarnheden A, Lindblad M, Lindsten K et al (2002) Genetic diversity of Wheat dwarf virus. Arch Virol 147:205–216
Lemmetty A, Huusela-Veistola E (2005) First report of WDV in winter wheat in Finland. Plant Dis 89:912. https://doi.org/10.1094/PD-89-0912B
Lindbland M, Sandgren M, Sigvald R (1999) Epidemiology and control of wheat dwarf. In: Proc. VIIth Int. Plant Virus Epidemiology Symp. Aguadulce (Almeria), Spain, p 114
Lindbland M, Waern P (2002) Correlation of wheat dwarf incidence to winter wheat cultivation practices. Agric Ecosyst Environ 92(2–3):115–122. https://doi.org/10.1016/S0167-8809(01)00302-4
Lindsten K, Lindsten B (1993) Occurrence and transmission of Wheat dwarf virus (WDV) in France. In: A.N.P.P. Third Int. Conf. on Pests in Agriculture. Montpellier, France, pp 41–48
Lindsten K, Vacke J (1991) A possible barley adapted strain of Wheat dwarf virus (WDV). Acta Phytopathol Entomol Hung 26:175–180
Liu Y, Wang B, Vida G, Cséplo-Károlyi M, Wu B, Wu Y, Wang X (2012) Genomic analysis of the natural population of Wheat dwarf virus in wheat from China and Hungary. J Integr Agric 11(12):2020–2027
Lotfipour M, Amid Motlagh MH, Afsharifar A, Behjatnia SAA, Izadpanah K (2013) The nucleotide sequence of complete genome and taxonomic position of wheat and barley isolates of wheat strain of wheat dwarf virus in Iran. Iran J Plant Pathol 49:375–388
MacDowell SW, Macdonald H, Hamilton WD, Coutts RH, Buck KW (1985) The nucleotide sequence of cloned wheat dwarf virus DNA. EMBO J 4(9):2173–2180
Marn MV, Plesko IM (2017) First report of the occurrence of Wheat dwarf virus infecting wheat in Slovenia. Plant Dis 101(7):1336. https://doi.org/10.1094/PDIS-11-16-1642-PDN
Mishchenko LT (2009) Viral disease of winter wheat. Phytosociocenter, Kyiv (in Ukrainian)
Mishchenko LT, Dunich AA, Mishchenko IA, Petrenkova VP, Mukha TI (2018) Monitoring of economically important wheat viruses under weather conditions change in Ukraine and investigation of seed transmission of Wheat streak mosaic virus. Bulg J Agric Sci 24(4):660–669
Muhire B, Martin DP, Brown JK, Navas-Castillo J, Moriones E, Zerbini FM, Rivera-Bustamante R, Malathi VG, Briddon RW, Varsani A (2013) A genome-wide pairwise-identity-based proposal for the classification of viruses in the genus Mastrevirus (family Geminiviridae). Arch Virol 158:1411–1424. https://doi.org/10.1007/s00705-012-1601-7
Muhire BM, Varsani A, Martin DP (2014) SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS ONE 9(9):e108277. https://doi.org/10.1371/journal.pone.0108277
Najar A, Othman FB, Boudhir H, Makkouk KM, Zarouk R, Bessai R, Kumari SG (2000) Viral diseases of cultivated legume and cereal crops in Tunisia. Phytopathol Mediterr 39:423–432
Ng JCK, Falk BW (2006) Virus-vector interactions mediating nonpersistent and semipersistent transmission of plant viruses. Ann Rev Phytopathol 44:183–212. https://doi.org/10.1146/annurev.phyto.44.070505.143325
Procedure for examination of state trials of cereals, grain-legume crops, groat crops. Protection of rights for plant varieties, Kyiv 2003, issue 2, 241 p (in Ukrainian)
Ramsell JNE, Lemmetty A, Jonasson J, Andersson A, Sigvald R, Kvarnheden A (2008) Sequence analyses of Wheat dwarf virus isolates from different hosts reveal low genetic diversity within the wheat strain. Plant Pathol 57:834–841. https://doi.org/10.1111/j.1365-3059.2008.01862.x
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Schubert J, Habekus A, Kazmaier K, Jeske H (2007) Surveying cereal-infecting geminiviruses in Germany—diagnostics and direct sequencing using rolling circle amplification. Virus Res 127:61–70. https://doi.org/10.1016/j.virusres.2007.03.018
Schubert J, Habekuss A, Wu B, Thieme T, Wang X (2014) Analysis of complete genomes of isolates of the Wheat dwarf virus from new geographical locations and descriptions of their defective forms. Virus Genes 48(1):133–139. https://doi.org/10.1007/s11262-013-0989-9
Shepherd DN, Martin DP, McGivern DR, Boulton MI, Thomson JA, Rybicki EP (2005) A three-nucleotide mutation altering the Maize streak virus Rep pRBRinteraction motif reduces symptom severity in maize and partially reverts at high frequency without restoring pRBR-Rep binding. J Gen Virol 86:803–813. https://doi.org/10.1099/vir.0.80694-0
Širlová L, Vacke J, Chaloupková M (2005) Reaction of selected winter wheat varieties to autumnal infection with Wheat dwarf virus. Plant Prot Sci 41:1–7. https://doi.org/10.17221/2732-PPS
Sõmera M, Truve E, Sooväli P (2019) First report of Wheat dwarf virus in winter wheat in Estonia. Dis Notes 103(7):1797. https://doi.org/10.1094/PDIS-11-18-2059-PDN
Stephanov J, Dimov A (1981) Bolestta vdjudjavanje po spenittsata Bolgaria. [The Wheat dwarf disease in Bulgaria]. Rasteniev Nauki 18:1274–2128 (in Bulgarian)
Tóbiás I, Kiss B, Bakardjieva N, Palkovics L (2009) The nucleotide sequence of barley strain of of Wheat Dwarf Virus isolated in Bulgaria. Cereal Res Commun 37:237–242. https://doi.org/10.1556/CRC.37.2009.2.10
Tóbiás I, Shevchenko O, Kiss B, Bysov A, Snihur H, Polischuk V, Salánki K, Palkovics L (2011) Comparison of the nucleotide sequences of wheat dwarf virus (WDV) isolates from Hungary and Ukraine. Pol J Microbiol 60(2):125–131
Trzmiel K (2018) Detection and discrimination of barley and wheat specific form of WDV. Cereal Res Commun 46(2):301–310. https://doi.org/10.1556/0806.46.2018.11
Vacke J (1961) Wheat dwarf virus disease. Biol Plant Praha 3:228–233
Vacke J, Cibulka R (2000) Response of selected winter wheat varieties to wheat dwarf virus infection at an early growth stage. Czech J Genet Plant Breed 36:1–4
Wu B, Shang X, Schubert J, Habekuß A, Elena SF, Wang X (2015) Global-scale computational analysis of genomic sequences reveals the recombination pattern and coevolution dynamics of cereal-infecting geminiviruses. Sci Rep 5:8153. https://doi.org/10.1038/srep08153
Xie J, Wang X, Liu Y, Peng Y, Zhou G (2007) First report of the occurrence of WDV in wheat in China. Plant Dis 91:111. https://doi.org/10.1094/PD-91-0111B
Zhang P, Liu Y, Liu W, Massart S, Wang X (2014) Simultaneous detection of wheat dwarf virus, northern cereal mosaic virus, barley yellow striate mosaic virus and rice black-streaked dwarf virus in wheat by multiplex RT-PCR. J Virol Methods 249:170–174. https://doi.org/10.1016/j.jviromet.2017.09.010
Acknowledgements
The authors acknowledge Oleg Skufinskyi of the "Agrotorg-Podillya" for providing samples of diseased wheat and significant interest in the work.
Funding
Not applicable.
Author information
Authors and Affiliations
Contributions
LM designed the study; LM, AD, AD, OM and IM prepared material for the experiments and collected data; LM, AD, AD and TK conducted the experiments; AD wrote the manuscript. All authors analyzed all data, drafted the figures, commented, reviewed and approved the final manuscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Consent for publication
All authors declare consent of publication.
Consent to participate
Approved by all authors.
Ethics approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Mishchenko, L.T., Dunich, A.A., Mishchenko, I.A. et al. Wheat dwarf virus in Ukraine: occurrence, molecular characterization and impact on the yield. J Plant Dis Prot 129, 107–116 (2022). https://doi.org/10.1007/s41348-021-00552-w
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s41348-021-00552-w