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Microbiological study and insecticidal potential of purified extract from Streptomyces sp. on the larvae of Galleria mellonella

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Abstract

In the present study, one Streptomyces species was isolated from a soil sample that was collected in Khafr El Shikh Governorate. Actinomycetes are prolific producers of antibiotics and important suppliers to the pharmaceutical industry. They can produce a wide range of secondary metabolites. The isolate was purified and characterized by morphological, physiological, biochemical methods and using Scanning electron microscope (SEM). The isolate was found to be Streptomyces catenula like microorganism and was therefore called Streptomyces catenula MY 12. The Streptomyces metabolite was extracted using ethyl acetate solvent. The purified extract was used to study the effect against 3rd instar larvae of the greater wax moth, Galleria mellonella. The study showed deleterious effects on growth and development of G. mellonella larvae. The results demonstrated that the mortality increased with increase in concentration. The extract prolonged the larval—pupal periods and reduced both pupation and percentage of adult emergence. The LC50 and LC90 values were calculated. The consumed food by unirradiated and irradiated 3rd instar larvae of G. mellonella in each concentration was studied. The larvae consumed much media in control treatment and fewer amounts in the higher concentration of the extract. The SEM revealed that different malformation or abnormalities were observed in larval cuticle and associated sensilla in the form of tear and ridged tergum cuticle. Also, broken and fallen or scarcity of trichodea sensilla. Undistinguished segments tear sternum and destroy plural membrane. The spiracles were completely closed, and prolegs crochets disappeared.

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Authors and Affiliations

  1. Department of Radiation Microbiology, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

    Mahmoud Abdel Wahab Soliman

  2. Department of Natural Products Research, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

    Ali Fawzy Hamza & Nagwan Fahmy Zahran

  3. Chemistry Department, Faculty of Engineering, Ain Shams University, Cairo, Egypt

    Ghada Bassioni

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  1. Mahmoud Abdel Wahab Soliman
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  2. Ali Fawzy Hamza
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  3. Nagwan Fahmy Zahran
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  4. Ghada Bassioni
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All authors have contributed equally to the work.

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Correspondence to Nagwan Fahmy Zahran.

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Soliman, M.A.W., Hamza, A.F., Zahran, N.F. et al. Microbiological study and insecticidal potential of purified extract from Streptomyces sp. on the larvae of Galleria mellonella. J Plant Dis Prot 128, 1565–1574 (2021). https://doi.org/10.1007/s41348-021-00508-0

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