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Genetic Diversity of Scanty Available Himalayan Saussurea obvallata (DC.) Edgew.

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Abstract

Saussurea obvallata (DC.) Edgew. of Asteraceae family is an endangered plant species used for traditional, cultural and religious purposes. The species is not systematically explored till date at global level. Here, we show the genetic variation and gene flow evaluation within and among the population of S. obvallata from different sites of Uttarakhand region of India by utilizing ten ISSR primers. The result showed 59 polymorphic loci in 30 studied individuals and 100% polymorphism in all the fragments. Number of alleles for each locus (Na), Shannon’s index (I), number of effective alleles per locus (Ne) and Nei’s gene diversity (H) were calculated to be 1.59, 1.52, 0.38 and 0.27, respectively. The mean polymorphic loci percentage (Pp%) was found 60%, whereas genetic differentiation level (Gst) amid population were 39.37% and 60.63% genetic variation found among and within populations, respectively. The molecular variance partitioning by AMOVA demonstrated a noteworthy genetic differentiation within (66%) and among (44%) populations. The study revealed the lowest gene flow level among all studied populations of S. obvallata; therefore, the use of modern biotechnological interventions is recommended for conservation and sustainable utilization of this valuable species.

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Acknowledgements

We would like to thank Dr. Bheem Joshi (Wildlife Institute of India, Dehradun) and Dr. Praveen Dhyani (G.B. Pant National Institute, Almora, India) for their great help and technical support during this research. Help and support also received from the Graphic Era (Deemed to be University), Dehradun, India, are also duly acknowledged.

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This research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Biotechnology, Graphic Era (Deemed to be) University, 566/6 Bell Road Clement Town, Dehra Dun, Uttarakhand, 248 001, India

    Prabhakar Semwal, Sakshi Painuli & L. M. S. Palni

  2. G.B. Pant National Institute of Himalayan Environment and Development, Garhwal Unit, Srinagar, Uttarakhand, 246 174, India

    Arun Jugran

  3. Department of Botany, School of Biosciences and Biotechnology, Baba Ghulam Shah Badshah University, Rajouri, J&K, 185 131, India

    Susheel Verma

  4. Department of Life Sciences, Graphic Era (Deemed to be) University, 566/6 Bell Road Clement Town, Dehradun, Uttarakhand, 248 001, India

    Ashish Thapliyal

  5. Department of Pharmacognosy, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India

    Devesh Tewari

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  1. Prabhakar Semwal
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  2. Sakshi Painuli
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Semwal, P., Painuli, S., Jugran, A. et al. Genetic Diversity of Scanty Available Himalayan Saussurea obvallata (DC.) Edgew.. Iran J Sci Technol Trans Sci 44, 587–594 (2020). https://doi.org/10.1007/s40995-020-00862-y

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