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Early stage of obesity potentiates nitric oxide reduction during the development of renal failure

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Abstract

Background

Obesity is a serious health problem associated with the pathogenesis of various metabolic diseases. Nitric Oxide (NO) plays an important role in kidney function and altered NO levels have been associated with the pathogenesis of obesity. Therefore, we aimed to study whether an early stage of obesity contributes with progression of renal failure through further NO impairment.

Methods

Male C57BL/6 mice were fed with a high-fat diet (HFD) or a normal diet (ND) during 2 weeks. All mice underwent either sham surgery (sham) or 5/6 nephrectomy (Np). One group of HFD Np mice was treated with antioxidants plus L-arginine. Kidney damage parameters were assessed and eNOS metabolism was evaluated.

Results

Mice on a HFD increased body weight, eNOS protein and mRNA expression, and radical oxygen species (ROS). Urine nitrites excretion, urine volume, and plasma BH4 were decreased. In HFD mice, 5/6 Np further increased BH2 and urine protein concentration, ROS levels, and eNOS mRNA expression. The decrease in BH4 plasma levels and urine nitrites excretion was accentuated. NO synthesis stimulation with the antioxidants + L-arginine treatment prevented all these changes.

Conclusions

The early changes in NO metabolism are associated with an early stage of obesity. This effect on NO potentiates kidney damage development.

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Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. CINVESTAV Monterrey, Apodaca, Nuevo León, Mexico

    Ana María Gámez-Méndez, Hilda Vargas-Robles, Mónica Arellano-Mendoza, Erika Cruz-Laguna, Amelia Rios & Bruno Escalante

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  1. Ana María Gámez-Méndez
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  2. Hilda Vargas-Robles
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  3. Mónica Arellano-Mendoza
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  4. Erika Cruz-Laguna
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  5. Amelia Rios
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  6. Bruno Escalante
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Correspondence to Bruno Escalante.

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Gámez-Méndez, A.M., Vargas-Robles, H., Arellano-Mendoza, M. et al. Early stage of obesity potentiates nitric oxide reduction during the development of renal failure. J Nephrol 27, 281–287 (2014). https://doi.org/10.1007/s40620-013-0029-9

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  • DOI: https://doi.org/10.1007/s40620-013-0029-9

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