Skip to main content
SpringerLink
Log in

Biochemical changes in two Vigna spp. during drought and subsequent recovery

  • Original Article
  • Published:
Indian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Drought is one of the major environmental factors restricting crop production world over. The present experiment was conducted to evaluate the changes in important biochemical traits and yield of pulses under drought stress imposed during reproductive stage. Black gram (Vigna mungo L.) genotypes: T-9, PU-19 and green gram (Vigna radiata L.) genotypes: Pratap, TMB-37 were taken as test material in a randomized block design with three replications. Leaf chlorophyll content, nitrate reductase activity and total flavonoid were found to be positively correlated with soil moisture content, while negative correlations were obtained with proline and anthocyanin content. Significant differences among genotypes, treatments and interaction between the genotypes and treatment were observed for yield and other biochemical parameters, which demonstrated the existence of differences among the genotypes studied for drought tolerance. Genotypes T-9 of black gram and Pratap of green gram were found to be more tolerant to drought stress. Among the crops, black gram showed better drought tolerance than green gram. Biochemical parameters chlorophyll content, leaf proline content, nitrate reductase activity and anthocyanin content were identified as marker traits for selecting drought resistant pulse genotypes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Al Asbahil, A. A., AlMaqtari, M. A., & Mohammed, K. (2012). ABA biosynthesis defective mutants reduce some free amino acids accumulation under drought stress in tomato leaves in comparison with Arabidopsis plants tissues. Journal of Stress Physiology & Biochemistry, 8(2), 179–192.

    Google Scholar 

  • Alaei, Y. (2011). The effect of amino acids on leaf chlorophyll content in bread wheat genotypes under drought stress conditions. Middle-East Journal of Scientific Research, 10(1), 99–101.

    CAS  Google Scholar 

  • Ali, M., & Gupta, S. (2012). Carrying capacity of Indian agriculture: pulse crops. Current Science, 102(6), 874–881.

    Google Scholar 

  • Anderson, J. M., & Boardman, N. K. (1964). Studies on greening of dark grown bean plants. Australian Journal of Biological Science, 17, 93–101.

    Google Scholar 

  • Baroowa, B., & Gogoi, N. (2012). Effect of induced drought on different growth and biochemical attributes of black gram (Vigna mungo L.) and green gram (Vigna radiate L.). Journal of Environmental Research and Development, 6, 584–593.

    Google Scholar 

  • Bartels, D., & Sunker, R. (2005). Drought and salt tolerance in plants. Critical Reviews in Plant Sciences, 24, 23–58.

    Article  CAS  Google Scholar 

  • Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39, 205–207.

    Article  CAS  Google Scholar 

  • Chaves, M. M., Pereira, J. S., Maroco, J., Rodriques, M. L., Osorio, M. L., Carvatho, I., et al. (2002). How plants cope with water stress in the field photosynthesis and growth? Annals of Botany, 89, 907–916.

    Article  CAS  PubMed  Google Scholar 

  • Fresneau, C., Ghashghaie, J., & Cornic, G. (2007). Drought effect on nitrate reductase and sucrose-phosphate synthase activities in wheat (Triticum durum L.): role of leaf internal CO2. Journal of Experimental Botany, 58(11), 2983–2992.

    Article  CAS  PubMed  Google Scholar 

  • Hatier, J. H. B., & Gould, K. S. (2008). Foliar anthocyanins as modulators of stress signals. Journal of Theoretical Biology, 253, 625–627.

    Article  CAS  PubMed  Google Scholar 

  • Hossain, B. M., Rahman, W. M., Rahman, N. M., Noorulanwar, A. H. M., & Hossen, A. K. M. M. (2010). Effects of water stress on yield attributes and yield of different mungbean genotypes. International Journal of Sustainable Crop Production, 5(1), 19–24.

    Google Scholar 

  • Jaworski, E. (1971). Nitrate reductase assay in intact plant tissue. Biochemical Biophysical Research Communications, 43, 1274–1279.

    Article  CAS  PubMed  Google Scholar 

  • Jordan, B. R., James, P. E., Strid, A., & Anthony, R. G. (1994). The effect of ultraviolet-B radiation on gene expression and pigment composition in etiolated and green pea leaf tissue: UV-B induced changes are gene-specific and dependent upon the development stage. Plant Cell & Environment, 17, 45–54.

    Article  CAS  Google Scholar 

  • Kiani, S. P., Maury, P., Sarrafi, A., & Grieu, P. (2008). QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annus L.) under well-watered and water-stressed conditions. Plant Science, 175, 565–573.

    Article  Google Scholar 

  • Liu, K., Ye, Y., Tang, C., Wang, Z., & Yang, J. (2008). Responses of ethylene and ACC in rice grains to soil moisture and their relations to grain filling. Frontiers of Agriculture in China, 2(2), 172–180.

    Article  Google Scholar 

  • Long, S. P., Humphries, S., & Falkowski, P. G. (1994). Photo-inhibition of photosynthesis in nature. Annual Review of Plant Physiology and Plant Molecular Biology, 45, 633–662.

    Article  CAS  Google Scholar 

  • Merzlyak, M. N., Chivkunova, O. B., Solovchenko, A. E., & Naqvi, K. R. (2008). Light absorption by anthocyanins in juvenile, stressed, and senescing leaves. Journal of Experimental Botany, 59, 3903–3911.

    Article  CAS  PubMed  Google Scholar 

  • Mohammadkhani, N., & Heidari, R. (2008). Drought-induced accumulation of soluble sugars and proline in two maize varieties. World Applied Sciences Journal, 3(3), 448–453.

    Google Scholar 

  • Okwu, D. E. (2004). The phytochemicals and vitamins contents of indigenous spices of South Eastern, Nigeria. Journal of Sustainable Agriculture and Environment, 6, 30–34.

    Google Scholar 

  • Osuagwu, G. G. E. (2008). The effect of rate of application of poultry manure on the phenol, flavonoid and steroid potential of the leaves of Ocimumgratissimum. Journal of Sustainable Agriculture and Environment, 10(2), 106–111.

    Google Scholar 

  • Pandey, H. C., Baig, M. J., & Bhatt, R. K. (2012). Effect of moisture stress on chlorophyll accumulation and nitrate reductase activity at vegetative and flowering stage in Avena species. Agricultural Science Research Journal, 2(3), 111–118.

    Google Scholar 

  • Sundaresan, S., & Sudhakaran, P. R. (1995). Water stress-induced alterations in proline metabolism of drought-susceptible and -tolerant cassava (Manihot esculenta) cultivars. Physiologia Plantarum, 94, 635–642.

    Article  CAS  Google Scholar 

  • Tahkokorpi, M. (2010). Anthocyanins under drought and drought-related stresses in bilberry (Vaccinium myrtillus L.). Oulu: Acta Universities Ouluensis.

    Google Scholar 

  • Tawfik, K. M. (2008). Effect of water stress on addition to potassiomag as application on mungbean. Australian Journal of basic and Applied Science, 2(1), 42–52.

    CAS  Google Scholar 

  • Yang, L., Han, Z. M., Yang, L. M., & Han, M. (2010). Effects of water stress on photosynthesis, biomass, and medicinal material quality of Tribulusterrestri. Chinese Journal of Applied Ecology, 21, 2523–2528.

    PubMed  Google Scholar 

  • Zaeifizade, M., & Goliov, R. (2009). The Effect of the Interaction between Genotypes and Drought Stress on the Superoxide Dismutase and Chlorophyll Content in Durum Wheat Landraces. Turkish Journal of Biology, 33, 1–7.

    Google Scholar 

Download references

Acknowledgments

Authors are thankful to University Grant Commission (UGC), New Delhi, India for providing financial support for this work and to the Chief Scientist, RARS (Shillongoni), Assam for providing us the seed material.

Author information

Author notes

  1. Nirmali Gogoi

    Present address: Department of Environmental Science, Tezpur University, Napaam, Tezpur, 784028, Assam, India

Authors and Affiliations

  1. Department of Environmental Science, Tezpur University, Napaam, Tezpur, 784028, Assam, India

    Bhaswatee Baroowa

Authors
  1. Bhaswatee Baroowa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nirmali Gogoi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nirmali Gogoi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baroowa, B., Gogoi, N. Biochemical changes in two Vigna spp. during drought and subsequent recovery. Ind J Plant Physiol. 18, 319–325 (2013). https://doi.org/10.1007/s40502-013-0048-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40502-013-0048-5

Keywords

Search

Navigation