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Laccase from Aspergillus nidulans TTF6 Showing Pb Activation for Smaller Substrates and Dyes Remediation in All Climates

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Prompted by multiple applications of laccases, the fungi yielding enzyme with wider geographical applicability and other novel features was explored from Jaisalmer desert (India). One of the isolates TTF6 yielded laccase exhibiting novel many-fold amplification in activity in the presence of lead (Pb) and tolerance to Na. The peroxide-free enzyme showed apparent molecular mass 40 kDa, activity from 10 to 80 °C, temperature optima at 60 °C and pH optima at 5.0. It was highly thermostable losing only 22 and 44% of its maximal activity in 24 and 48 h, respectively, with a half-life of 60 h at 60 °C. It was found resistant to studied metals including Cu and Zn and showed tolerance to many organic solvents. The enzyme was produced best in Olga medium and induced by ethanol and copper sulfate but not by guaiacol, though guaiacol could be utilized as substrate. The partially purified enzyme decolorized selected synthetic dyes at 20–40 °C, the decolorization rate, however, was not affected by Pb. The fungus was identified on the basis of morphological and ITS sequence characteristics as A. nidulans. This is the first report of a Pb-activated laccase and the sixth phenol oxidase from A. nidulans reported so far.

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Acknowledgements

A fruitful discussion during screening of laccase producing fungus with Dr. Harald Claus, Institut fúr Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, Mainz, Germany, is gratefully acknowledged herewith. The work was supported by Madhya Pradesh Council of Science and Technology (Grant Number 3902/CST/R&D/2011) to the corresponding author.

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Authors and Affiliations

  1. Government Science and Commerce College, Benazir, Bhopal, MP, India

    Vinod Chaurse & Deepak Chauhan

  2. Government Postgraduate College, Biaora, MP, India

    Sanjay Sahay

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  1. Sanjay Sahay
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  2. Vinod Chaurse
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Correspondence to Sanjay Sahay.

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Significance Statement

TTF6 laccases with tolerance to various inhibitory conditions, residual activity at wider temperature range and novel Pb activation for smaller substrates (ABTS) not larger substrates (dyes) are opening new vistas in laccases’ science and applications.

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Sahay, S., Chaurse, V. & Chauhan, D. Laccase from Aspergillus nidulans TTF6 Showing Pb Activation for Smaller Substrates and Dyes Remediation in All Climates. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 143–150 (2020). https://doi.org/10.1007/s40011-019-01092-y

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