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Screening Arbuscular Mycorrhizal Fungi in Order to Select the Best for Alleviating Wilt Disease Complex of Capsicum

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Abstract

Arbuscular mycorrhizal fungi (AMF) play an important role in protection of host plant from infection by soil-borne plant pathogens. In the present study different AMF were tested for their ability to alleviate wilt disease complex of tomato caused by bacterial pathogen Ralstonia solanacearum, fungal pathogen Phytophthora capsici and root-knot nematode Meloidogyne incognita. Plant growth parameters like plant height, stem girth, biovolume index, root and shoot dry weight, total plant dry weight, disease index and mycorrhizal parameters like per cent root colonization and spore count in the root zone soil were evaluated. In general AMF inoculation increased plant growth parameters. Among 11 AMF screened, Glomus bagyarajii was found to be the best AMF in enhancing plant height, stem girth, total plant dry weight, mycorrhizal root colonization and most importantly exhibited least per cent disease index (50.20 %) as compared to control (82.14 %). The results show the potential of using G. bagyarajii for the biocontrol of wilt disease complex of capsicum.

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References

  1. Sreedhara DS, Kerutagi MG, Basavaraja H, Kunnal LB, Dodamani MT (2013) Economics of capsicum production under protected conditions in Northern Karnataka. Karnataka J Agric Sci 26:217–219

    Google Scholar 

  2. Di Vito M, Greco N, Carella A (1985) Population densities of Meloidogyne incognita and yield of Capsicum annuum. J Nematol 17:45–49

    PubMed  PubMed Central  CAS  Google Scholar 

  3. Rao MS, Ramachandran N, Sowmya DS, Ratnamma K, Chaya MK, Manoj Kumar R (2009) Biological control of nematode induced disease complex in certain vegetable crops. In: Abstracts of international conference on horticulture, Bangalore, India, p 213, 9–12 November 2009

  4. Shali S (2000) Studies on chilli wilt in Jammu. M.Sc Thesis submitted to Sher-e-Kashmir University of Agriculture Science and Technology (Kashmir) Shalimar Campus, Srinagar Jammu and Kashmir, India

  5. Najar AG (2001) Cause and management of chilli wilt in Kashmir. Ph.D thesis submitted to Sher-e-Kashmir University of Agricultural Science and Technology (Kashmir) Shalimar Campus, Srinagar Jammu and Kashmir India

  6. Azcon-Aguilar C, Barea JM (1996) Arbuscular mycorrhizas and biocontrol of soil-borne plant pathogens-an overview of the mechanisms involved. Mycorrhiza 6:457–464

    Article  Google Scholar 

  7. Tahat MM, Sijam K, Othman R (2010) Mycorrhizal fungi as a biocontrol agent. Plant Pathol J 9:13085–13094

    Google Scholar 

  8. Chandanie WA, Kubota M, Hyakumachi M (2009) Interactions between the arbuscular mycorrhizal fungus Glomus mosseae and plant growth promoting fungi and their significance for enhancing plant growth and suppressing damping-off of cucumber (Cucumis sativus L.). Appl Soil Ecol 41:336–341

    Article  Google Scholar 

  9. Fierro-Corondao RA, Castro-Moreno MG, Ruelas-Ayala RD, Apodaca-Sanchez MA, Maldonado-Mendoza IE (2013) Induced protection by Rhizophagus intraradices against Fusarium wilt of tomoato. Interciencia 38:48–53

    Google Scholar 

  10. Bagyaraj DJ (1996) Mycorrhizal symbiosis in tropical trees in relation to control of root pathogens. In: Nair KSS, Sharma PK, Varma RV (eds) Impact of diseases and insect pests. Kerala Forest Research Institute Publishers, Peechi, pp 239–245

    Google Scholar 

  11. Habte M, Schmitt D (2005) Antagonistic interactions between plant parasitic nematode and arbuscular mycorrhizal fungi. In: Mehrotra VS (ed) Mycorrhizae: role and application. Allied publishers, New Delhi, pp 183–203

    Google Scholar 

  12. Lekshmi MS, Ram AB, Bagyaraj DJ (2014) Biocontrol of wilt disease complex of tomato by arbuscular mycorrhizal fungi. J Soil Biol Ecol 34:128–139

    Google Scholar 

  13. Rao MS, Sowmya DS, Chaya MK, Manoj Kumar R, Rathnamma K, Gavaskar J, Priti K, Ramachandran N (2012) Management of nematode induced wilt disease complex in Capsicum using Pseudomonas fluorescens and Paecilomyces lilacinus. Nematol Mediterr 40:101–105

    Google Scholar 

  14. Jackson ML (1973) Soil chemical analysis. Prentice Hall of India, New Delhi, pp 239–241

    Google Scholar 

  15. Gerdemann JW, Nicolson TH (1963) Spores of mycorrhizal Endogone species extracted from soil by wet-sieving and decanting. Trans Br Mycol Soc 46:235–244

    Article  Google Scholar 

  16. Hatchell GE (1985) Production of bare root seedlings. In: Proceedings of 3rd Bio South SI Res Conf, pp 395–402

  17. Philips JH, Hayman DS (1970) Improved procedures for cleaning roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–161

    Article  Google Scholar 

  18. Cobb NA (1918) Estimating the nematode population of soil, US Department of Agriculture, Bureau of Pant Industry, Agriculture Technical Circular, vol 1, pp 1–48

  19. Schidler AF (1961) A simple substitute for a Baermann funnel. Plant Dis Rep 45:747–748

    Google Scholar 

  20. Kesavan V, Chowdhary B (1977) Screening for resistant Fusarium wilt in tomato. SABRAOJ 21:57–65

    Google Scholar 

  21. Little TM, Hills FC (1978) Agricultural exprimentation. Wiley, New York

    Google Scholar 

  22. Oyetunji and Salami (2011) Control of Fusarium wilt in the stems on mycorrhizal and trichodermal inoculated pepper. J Appl Biosci 45:3071–3080

    Google Scholar 

  23. Tahat MM, Sijam K, Othman R (2012) The potential of endomycorrhizal fungi in controlling tomato bacterial wilt Ralstonia solanacearum under glasshouse conditions. Afr J Biotechnol 11:13085–13094

    Google Scholar 

  24. Thilagar G, Bagyaraj DJ (2015) Influence of different arbuscular mycorrhizal fungi on growth and yield of chilly. Proc Natl Acad Sci India Sect B Biol Sci 85:71–75

    Article  CAS  Google Scholar 

  25. Puttaradder J, Lakshman HC (2015) Effect of co-inoculation of AM fungi on growth, P uptake, acid and alkaline phosphatase activity in Capsicum annum L. Var. Pusa jwala. Int J Res Eng Appl Sci 6:91–98

    Google Scholar 

  26. Filion M, St-Arnaud M, Jabaji-Hare SH (2003) Quantification of Fusarium soloni f. sp. Phaseoli in mycorrhizal bean plants and surrounding mycorrhizosphere soil using real-time polymerase chain reaction and direct isolations on selective media. Phytopathology 93:229–235

    Article  PubMed  CAS  Google Scholar 

  27. Whipps JM (2004) Prospects and limitations for mycorrhizas in biocontrol of root pathogens. Can J Bot 82:1198–1227

    Article  Google Scholar 

  28. Pavithra S, Ravichandra NG, Suresh SR (2014) Evaluation of brinjal Genotypes against Meloidogyne incognita and Ralstonia solanacearum. Trends Biosci 7:3932–3935

    Google Scholar 

  29. Graham JH, Menge JA (1982) Influence of vesicular arbuscular mycorrhizae and soil phosphorus on take-all disease of wheat. Phytopathology 72:95–98

    Article  Google Scholar 

  30. Ren L, Lou Y, Sakamoto K, Inubushi K, Amemiya Y, Shen Q, Xu G (2010) Effects of arbuscular mycorrhizal colonization on microbial community in rhizosphere soil and Fusarium wilt disease in tomato. Commun Soil Sci Plant 41:1399–1410

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are thankful to Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi (grant no. BT/PR 10623/AGR/05/421/2008) for the financial assistance to carry out this work.

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Authors and Affiliations

  1. Centre for Natural Biological Resources and Community Development (CNBRCD), 41 RBI Colony, Anand Nagar, Bangalore, 560024, India

    M. Raghavendra Kumar, R. Ashwin & D. J. Bagyaraj

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  1. M. Raghavendra Kumar
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  2. R. Ashwin
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  3. D. J. Bagyaraj
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Correspondence to D. J. Bagyaraj.

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Raghavendra Kumar, M., Ashwin, R. & Bagyaraj, D.J. Screening Arbuscular Mycorrhizal Fungi in Order to Select the Best for Alleviating Wilt Disease Complex of Capsicum. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 679–684 (2018). https://doi.org/10.1007/s40011-016-0804-1

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  • DOI: https://doi.org/10.1007/s40011-016-0804-1

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