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Plant growth promoting activities of rhizobacteria isolated from Podophyllum hexandrum growing in North-West regions of the Himalaya

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Abstract

A collection of 50 rhizospheric and 43 endorhizospheric bacteria was obtained from Podophyllum hexandrum grown in high hills of Churdhar, Himachal Pradesh, India and subjected to replica plating for one step screening of plant growth promoting (PGP) traits. After replica plating, 41 bacterial isolates simultaneously growing on Pikovskaya’s, Chrome-azurol-S, and Jensen’s medium were characterized for additional PGP traits. Of the total, 80.48 % isolates produced indole-3-acetic acid, 12.20 % produced HCN, 53.65 % exhibited chitinase and 41.46 % exhibited protease activity. In vitro inhibition of fungal pathogens viz., Alternaria solani, Fusarium oxysporum, Dematophora necatrix, Sclerotium rolfsii and Phytophthora spp. ranged from 44 % to 88 %. Twenty most efficient PGPR under in vitro conditions were evaluated for growth promotion of tomato in growth chamber. Significantly highest root length (43.00 mm), shoot length (73.00 mm), plant dry weight (12.90 mg plant−1) and vigour index (10,567.60) were recorded for isolate 2a1. The efficacy of isolate 2a1 was further evaluated under net house and resulted in remarkable increase in root length (58.02 %), shoot length (66.89 %), root dry weight (98.67 %), shoot dry weight (88.58 %), available nitrogen (31.89 %), phosphorus (22.15 %) and potassium (4.51 %) content in soil over untreated control. Remarkable increase was also recorded in nitrogen (11.85 %), phosphorus (23.08 %) and potassium (36.84 %) content of shoot biomass over control. Strain 2a1 was identified as Bacillus subtilis after biochemical and phylogenetic analysis. These results demonstrated that strain 2a1 from Podophyllum hexandrum exhibited multiple PGP attributes for development as a biofertilizer to enhance soil fertility and promote plant growth.

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Acknowledgments

The authors would like to thank ICAR (AINP on Soil Biodiversity and Biofertilizer), New Delhi, India, for providing the financial support. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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  1. Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, H.P., 173230, India

    Rashmi Sharma, Parul Sharma, Anjali Chauhan & C. K. Shirkot

  2. DAV University, Jalandhar, Punjab, 144012, India

    Abhishek Walia

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Correspondence to C. K. Shirkot.

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Sharma, R., Sharma, P., Chauhan, A. et al. Plant growth promoting activities of rhizobacteria isolated from Podophyllum hexandrum growing in North-West regions of the Himalaya. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 1443–1457 (2017). https://doi.org/10.1007/s40011-016-0722-2

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