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Variations in Pentacyclic Triterpenoids in Different Parts of Four Ocimum Species Using Reverse Phase-High Performance Liquid Chromatography

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Betulinic acid (BA), oleanolic acid (OA) and ursolic acid (UA) are the important pentacyclic triterpenoid compounds known for their anti-inflammatory, trypanocidal, antirheumatic, antiviral, antioxidant, antitumoral and many other activities. The quantitative analysis and distribution of BA, OA, and UA in different parts viz leaf, stem, and inflorescence of Ocimum basilicum (OB), O. gratissimum (OG), O. kilimandscharicum (OK), O. tenuiflorum (OT) was performed using reverse phase-high performance liquid chromatography and further confirmed the presence of triterpenoids in OG by high performance thin layer chromatography analysis. Leaf of OG was rich in OA (0.141 ± 0.007 %) and UA (0.296 ± 0.015 %), while BA (0.043 ± 0.011 %) was higher in the inflorescence of OK. The yield of BA, OA and UA in OG and OK was very significant (p < 0.001) as compared with OB and OT. The content of UA varied (0.008 ± 0.004 to 0.296 ± 0.015 %) within different parts and species, but this content was higher as compared to OA and BA. This communication presents for the first time simultaneous quantification of pentacyclic triterpenoids (BA, OA and UA) in OB, OG, OK and OT.

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References

  1. Gill D, Soni N, Sagar B, Raheja S, Agrawal S (2012) Ocimum kilimandscharicum: a systematic review. J Drug Deliv Ther 2(3):45–52

    Google Scholar 

  2. Anonymous (1966) The wealth of India—raw materials, vol III. CSIR, New Delhi

    Google Scholar 

  3. Chang X, Alderson PG, Wright CJ (2009) Variation in the essential oils in different leaves of Basil (O. basilicum L.) at day time. Open Hortic J 2:13–16

    Article  CAS  Google Scholar 

  4. Joshi RK (2013) Chemical composition, in vitro antimicrobial and antioxidant activities of the essential oils of Ocimum gratissimum, O. sanctum and their major constituents. Indian J Pharm Sci 75:457–462

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Vasudevan P, Kashyap S, Sharma S (1999) Bioactive botanicals from Basil (Ocimum sp.). J Sci Ind Res 58:332–338

    CAS  Google Scholar 

  6. Joshi RK (2013) Chemical composition of the essential oil of camphor basil (Ocimum kilimandscharicum Guerke). Glob J Med Plant Res 1:207–209

    Google Scholar 

  7. Suanarunsawat T, Ayutthaya WDN, Songsak T, Rattanamahaphoom J (2009) Anti-lipidemic actions of essential oil extracted from Ocimum sanctum L. leaves in rats fed with high cholesterol diet. J Appl Biomed 7:45–53

    CAS  Google Scholar 

  8. Joshi RK (2014) Chemical composition and antimicrobial activity of the essential oil of Ocimum basilicum L. (sweet basil) from Western Ghats of North West Karnataka, India. Anc Sci Life 33:149–154

    Article  Google Scholar 

  9. Joshi RK, Hoti SL (2014) Chemical composition of the essential oil of Ocimum tenuiflorum L. (Krishna Tulsi) from North West Karnataka, India. Plant Sci Today 1:99–102

    Article  Google Scholar 

  10. Vetal MD, Lade VG, Rathod VK (2012) Extraction of ursolic acid from Ocimum sanctum leaves: kinetics and modeling. Food Bioprod Process 90:793–798

    Article  CAS  Google Scholar 

  11. Anandjiwala S, Kalola J, Rajani M (2006) Quantification of eugenol, luteolin, ursolic acid, and oleanolic acid in black (Krishna tulasi) and green (Sri tulasi) varieties of Ocimum sanctum Linn. using high-performance thin-layer chromatography. J Assoc Anal Chem Int 89:1467–1474

    CAS  Google Scholar 

  12. Silva MGV, Vieira IGP, Mendes FNP, Albuquerque IL, dos Santos RN, Silva FO (2008) Variation of ursolic acid content in eight Ocimum species from northeastern Brazil. Molecules 13:2482–2487

    Article  CAS  PubMed  Google Scholar 

  13. Shanmuga SR, Gowtham L, Ramanathan M, Manikandan P, Venugopal V, Kamalakannan D (2011) Quantification of bioactive principles in Indian traditional herb Ocimum Sanctum Linn. (Holy Basil) leaves by high performance liquid chromatography. Asian J Biomed Pharm Sci 1:35–41

    Google Scholar 

  14. Shanmuga RS, Ramanathan M, Rajesh R, Satheesh B, Saravanan D (2012) LC-MS quantification of rosmarinic acid and ursolic acid in the Ocimum sanctum Linn. leaf extract (holy basil, tulsi). J Liq Chromatogr Relat Technol 35:634–650

    Article  Google Scholar 

  15. Verma S, Kothiyal P (2012) Pharmacological activities of different species of tulsi. Int J Biopharm Phytochem Res 1:21–39

    Google Scholar 

  16. Rout KK, Singh RK, Barik DP, Mishra SK (2012) Thin-layer chromatographic separation and validated HPTLC method for quantification of ursolic acid in various Ocimum species. J Food Drug Anal 20:865–871

    CAS  Google Scholar 

  17. Phadtare S, Pandit R, Shinde V, Mahadik K (2013) Comparative phytochemical and pharmacological evaluations of two varieties of Ocimum basilicum for antiarthritic activity. J Pharmacogn Phytochem 2:158–167

    Google Scholar 

  18. Pai SR, Pawar NV, Nimbalkar MS, Kshirsagar PR, Kolar FK, Dixit GB (2013) Seasonal variation in content of camptothecin from the bark of Nothapodytes nimmoniana (Grah.) Mabb., using HPLC analysis. Pharmacogn Res 5:219–223

    Article  Google Scholar 

  19. Pai SR, Nimbalkar MS, Pawar NV, Dixit GB (2011) Optimization of extraction techniques and quantification of Betulinic acid (BA) by RP-HPLC method from Ancistrocladus heyneanus Wall. Ex Grah. Ind Crops Prod 34:1458–1464

    Article  CAS  Google Scholar 

  20. Pai SR, Joshi RK (2014) Distribution of betulinic acid in plant kingdom. Plant Sci Today 1:103–107

    Article  Google Scholar 

  21. Yogeeswari P, Sriram D (2005) Betulinic acid and its derivatives: a review on their biological properties. Curr Med Chem 12:657–666

    Article  CAS  PubMed  Google Scholar 

  22. Nangia-Makker P, Tait L, Shekhar MPV, Palomino E, Hogan V, Piechocki MP (2007) Inhibition of breast tumor growth and angiogenesis by a medicinal herb: Ocimum gratissimum. Int J Cancer 121:884–889

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Balanehru S, Nagarajan B (1991) Protective effect of oleanolic acid and ursolic acid against lipid peroxidation. Biochem Int 24:981–990

    CAS  PubMed  Google Scholar 

  24. Pai SR, Upadhya V, Hegde HV, Joshi RK, Kholkute SD (2014) New report of triterpenoid betulinic acid along with oleanolic acid from Achyranthes aspera by reversed phase-ultra flow liquid chromatographic analysis and confirmation using high performance thin layer chromatographic and fourier transform-infrared spectroscopic techniques. J Planar Chromatogr 27:38–41

    Article  Google Scholar 

  25. Naumoska K, Simonovska B, Albret A, Vovk I (2013) TLC and TLC-MS screening of ursolic, oleanolic and betulinic acids in plant extracts. J Planar Chromatogr 26:125–131

    Article  CAS  Google Scholar 

  26. Hingorani L, Ebersole B, Patel S (2012) Optimization and validation of ursolic acid by HPLC in Ocimum sanctum. Planta Med 78:PJ17

    Google Scholar 

  27. Gupta SK, Prakash J, Srivastava S (2002) Validation of traditional claim of Tulsi, Ocimum sanctum Linn. as a medicinal plant. Indian J Exp Biol 40:765–773

    CAS  PubMed  Google Scholar 

  28. Joshi RK, Badakar VM, Kholkute SD, Khatib N (2011) Chemical composition and antimicrobial activity of the essential oil of the leaves of Feronia elephantum (Rutaceae) from North West Karnataka. Nat Prod Commun 6:141–143

    CAS  PubMed  Google Scholar 

  29. Joshi RK (2013) Pulegone and menthone chemotypes of Mentha spicata Linn. from Western Ghats region of North West Karnataka, India. Natl Acad Sci Lett 36:349–352

    Article  CAS  Google Scholar 

  30. Joshi RK (2014) 2,4,6-Trimethoxy-styrene new chemotype from the essential oil of Zanthoxylum ovalifolium Wight from India. Natl Acad Sci Lett 37:331–333

    Article  CAS  Google Scholar 

  31. Hanover JW (1966) Genetics of terpenes. I. Gene control of monoterpene levels in Pinus monticola Dougl. Heredity 21:73–84

    Article  CAS  Google Scholar 

  32. Mithen R, Raybould AF, Giamoustaris A (1995) Divergent selection for secondary matabolites between wild populations of Brassica oleracea and its implications for plant–herbivore interactions. Heredity 75:472–484

    Article  CAS  Google Scholar 

  33. Franz C (1993) Genetics. In: Hay RKM, Waterman PG (eds) Volatile oil crops. Longman, Harlow, pp 63–96

    Google Scholar 

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Acknowledgments

Authors are thankful to Indian Council of Medical Research (ICMR), New Delhi for providing facility during the work. SRP is indebted to the Department of Science and Technology (DST), New Delhi (Grant No. SB/YS/LS-71/2013) for financial support during the study.

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Authors and Affiliations

  1. Plant Biotechnology and Tissue Culture Division, Department of Plant Biotechnology and Tissue Culture, Regional Medical Research Centre (Indian Council of Medical Research), Nehru Nagar, Belgaum, Karnataka, 590 010, India

    Sandeep R. Pai

  2. Department of Phytochemistry, Regional Medical Research Centre (Indian Council of Medical Research), Nehru Nagar, Belgaum, Karnataka, 590 010, India

    Rajesh K. Joshi

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Correspondence to Rajesh K. Joshi.

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Pai, S.R., Joshi, R.K. Variations in Pentacyclic Triterpenoids in Different Parts of Four Ocimum Species Using Reverse Phase-High Performance Liquid Chromatography. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 1153–1158 (2017). https://doi.org/10.1007/s40011-015-0682-y

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