Skip to main content
SpringerLink
Log in

High-performance thin-layer chromatography profiling of Crateva magna (Lour.) DC. from different parts of South India

  • Original Research Paper
  • Published:
JPC – Journal of Planar Chromatography – Modern TLC Aims and scope Submit manuscript

Abstract

Phytochemicals derived from plants and plant-based substances have been used both in traditional medicine and in pharmaceutical products approved by the US Food and Drug Administration (FDA). Given the prevalence of lifestyle disorders and the constant emergence of newer pathogens, it is crucial to continue searching for potential drug candidates. Plant-based products are particularly promising due to their extensive chemical composition and reduced side effects. This study focused on Crateva magna (Lour.) DC., an underutilized plant species, obtained from various parts of South India, to evaluate its phytochemical composition. The extracts from C. magna contained alkaloids, flavonoids, phenols, and sterols and showed significant levels of total phenolics, flavonoids, and tannins, which are comparable to other related Crateva species. The methanolic and water extracts of the leaves showed overall more increased levels of phenolic and flavonoid content than bark extracts. On further scrutiny of the extracts using high-performance thin-layer chromatography (HPTLC), unique bands in addition to commonly shared bands were observed in extracts obtained from different locations. This variability emphasizes the effect of geoclimatic conditions on the secondary metabolite composition in plants. Further analysis and identification of marker compounds may pave the way for the discovery of new drugs to treat a variety of diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Abelson PH (1990) Medicine from plants. Science 247(4942):513. https://doi.org/10.1126/science.2300807

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Shu YZ (1998) Recent natural products based drug development: a pharmaceutical industry perspective. J Nat Prod 61(8):1053–1071. https://doi.org/10.1021/np9800102

    Article  CAS  PubMed  Google Scholar 

  3. Graul AI (2004) The year’s new drugs. Drug News Perspect 17(1):12–31

    Google Scholar 

  4. Bhattacharjee A, Shashidhara SC (2012) Phytochemical and ethno-pharmacological profile of Crateva nurvala Buch-Hum (Varuna): a review. Asian Pac J Trop Biomed 2:S1162–S1168. https://doi.org/10.1016/S2221-1691(12)60379-7

    Article  Google Scholar 

  5. Warrier PK (1998) Indian medicinal plants: a compendium of 500 species. Orient Longman, Hyderabad

    Google Scholar 

  6. Chidambaram K, Albert J, Karpagam K, Sivasubramanian N (2011) Antipyretic activity of Crateva magna bark in TAB-vaccine induced pyrexia. Int J Pharm Sci Res 2(4):856–859. https://doi.org/10.13040/IJPSR.0975-8232.2(4).856-59

    Article  Google Scholar 

  7. Abirami J, Jothi G, Brindha P (2017) Microscopic, physicochemical and phytochemical screening of Crateva magna (Lour.) DC. (Leaf). Int J Curr Pharm Res 9(5):201–204. https://doi.org/10.22159/ijcpr.2017v9i5.22564

    Article  CAS  Google Scholar 

  8. Meera R, Chidambaranathan N (2017) Anti cancer activity of ethanolic extract of Crateva magna Lour. (DC) against Ehrlich Ascitic carcinoma cell lines in mice. J Pharm Sci Res 9(10):1869–1873

    CAS  Google Scholar 

  9. Kumar S, Yadav A, Yadav M, Yadav JP (2017) Effect of climate change on phytochemical diversity, total phenolic content and in vitro antioxidant activity of Aloe vera (L.) Burm. f. BMC Res Notes. https://doi.org/10.1186/s13104-017-2385-3

    Article  PubMed  PubMed Central  Google Scholar 

  10. Kowalska T, Sajewicz M (2022) Thin-Layer Chromatography (TLC) in the screening of botanicals-its versatile potential and selected applications. Molecules 27(19):6607. https://doi.org/10.3390/molecules27196607

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Ram M, Abdin MZ, Khan MA, Jha P (2011) HPTLC Fingerprint Analysis: A Quality Control for Authentication of Herbal Phytochemicals. In: Srivastava M (ed) High-Performance Thin-Layer Chromatography (HPTLC). Springer, Berlin. https://doi.org/10.1007/978-3-642-14025-9_7

    Chapter  Google Scholar 

  12. Surayot P, Seekhaw P (2018) Phytochemical screening, determination of total phenolic and flavonoid contents and antioxidant activities from Crateva magna (Lour.) DC. Life Sci Environ J 19(2):297–305

    Google Scholar 

  13. Choudhary NK, Gupta K, Jain AK, Pal A (2020) Quantitative estimation of marker compound (Lupeol and Diosgenin) in polyherbal formulation by HPTLC method. Plant Arch 20(2):4337–4342

    Google Scholar 

  14. Radha SR, Doraiswamy R (2017) HPTLC fingerprinting analysis of the aqueous bark extract of Crateva magna Lour. (DC.) for terpenoid. World J Pharm Pharm Sci 6:1489–1495

    CAS  Google Scholar 

  15. Bazargani MM, Falahati-Anbaran M, Rohloff J (2021) Comparative Analyses of phytochemical variation within and between congeneric species of willow herb, Epilobium hirsutum and E. parviflorum: contribution of environmental factors. Front Plant Sci 11(595190):1–16. https://doi.org/10.3389/fpls.2020.595190

    Article  Google Scholar 

  16. Harbone JB (1984) Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. Springer, Dordrecht.

  17. Hendra R, Ahmad S, Oskoueian E, Sukari A, Shukor MY (2011) Antioxidant, Anti-inflammatory and Cytotoxicity of Phaleria macrocarpa (Boerl.) Scheff Fruit. BMC Complement Altern Med 11:110. https://doi.org/10.1186/1472-6882-11-110

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Nazarni R, Purnama D, Umar S, Eni H (2016) The effect of fermentation on total phenolic, flavonoid and tannin content and its relation to antibacterial activity in jaruk tigarun (Crateva nurvala, Buch HAM). Int Food Res J 23(1):309–315

    CAS  Google Scholar 

  19. Saeed N, Khan MR, Shabbir M (2012) Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med 12:221. https://doi.org/10.1186/1472-6882-12-221

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Wagay NA, Khan NA, Rothe SP (2017) Profiling of secondary metabolites and antimicrobial activity of Crateva religiosa G. Forst. Bark—A rare medicinal plant of Maharashtra India. Int J Biosci 10(5):343–354. https://doi.org/10.12692/ijb/10.5.343-354

    Article  Google Scholar 

  21. Nounagnon MS, Dah-nouvlessounon D, N’Tcha C, Legba B, Baba-Moussa F, Adjanohoun A, Baba-moussa L (2018) Phytochemistry and biological activities of Crateva adansonii extracts. Int J Pharm Pharm Sci 10(9):62–67. https://doi.org/10.22159/ijpps.2018v10i9.27197

    Article  CAS  Google Scholar 

  22. Kiruba S, Mahesh M, Paul ZM, Jeeva S (2011) Preliminary phytochemical screening of the pericarp of Crateva magna (Lour.) DC.—a medicinal tree. Asian Pac J Trop Biomed 1(1):S129–S130. https://doi.org/10.1016/S2221-1691(11)60139-1

    Article  Google Scholar 

  23. Christiana OA, Johnbull OE, Raphael CM, Joseph OO, Paul MO, Emmanuel GJ (2019) Gas Chromatographic study of bio-active compounds in methanolic extract of leaf of Crateva adansonii DC. J Phys Conf Ser 1299:012014. https://doi.org/10.1088/1742-6596/1299/1/012014

    Article  CAS  Google Scholar 

  24. Dah-Nouvlessounon D, Adoukonou-Sagbadja H, Diarrassouba N, Sina H, Adjanohoun A, Inoussa M, Akakpo D, Gbenou JD, Kotchoni SO, Dicko MH, Baba-Moussa L (2015) Phytochemical analysis and biological activities of Colanitida Bark. Biochem Res Int 2015:493879. https://doi.org/10.1155/2015/493879

    Article  PubMed  PubMed Central  Google Scholar 

  25. Lämke JS, Unsicker SB (2018) Phytochemical variation in treetops causes and consequences for tree-insect herbivore interactions. Oecologia 187:377–388. https://doi.org/10.1007/s00442-018-4087-5

    Article  ADS  PubMed  PubMed Central  Google Scholar 

  26. Meera R, Venkataraman S (2017) Preliminary phytochemical screening and HPTLC finger printing of root extract of Crateva magna Lour. (DC). Res J Pharm Biol Chem Sci 8(4):913–919

    CAS  Google Scholar 

Download references

Funding

None.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, India

    Shinsy Poongattil & Jibu Thomas

  2. Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Kerala, India

    Sulaiman Cheruthazhakkat

Authors
  1. Shinsy Poongattil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jibu Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sulaiman Cheruthazhakkat
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shinsy Poongattil.

Ethics declarations

Conflict of interest

All the authors declared that there is no conflict of interest to report.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 658 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Poongattil, S., Thomas, J. & Cheruthazhakkat, S. High-performance thin-layer chromatography profiling of Crateva magna (Lour.) DC. from different parts of South India. JPC-J Planar Chromat (2024). https://doi.org/10.1007/s00764-024-00294-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00764-024-00294-z

Keywords

Search

Navigation