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Enhanced Production of Nitrilase from Streptomyces sp. MTCC 7546 by Response Surface Method

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Nitrilases are enzymes with varied applications preferably used in preparation of industrially important compounds as well as in bioremediation processes. The production of enzymes is influenced by medium compositions as some of the constituents of medium directly affect the enzyme synthesis. In the present study, the components of the medium are optimized by classical and response surface methodology to maximize the production of intracellular nitrilase from Streptomyces sp. MTCC 7546. Glucose was observed as the most suitable carbon source for production of nitrilase by ‘one factor at a time’. The optimal concentration of glucose (5 g/L) was set as zero coded level for further optimization study. A central composite design was employed to study the effect of interaction among glucose, yeast extract and mineral base and evaluated for maximum nitrilase yield. The nitrilase production of 11.54 U/mg was observed at 24 h in medium containing 6.5 g/L glucose, 1.0 g/L yeast extract and 220 mL/L mineral base with 10 mM of benzonitrile as inducer. A twofold increment from 5.95 U/mg in the enzyme yield was observed after the optimization study of response surface methodology.

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References

  1. Gupta V, Gaind S, Verma PK, Sood N, Srivastava AK (2010) Purification and characterization of intracellular nitrilases from Rhodococcus sp.—potential role of periplasmic nitrilase. Afr J Microbiol Res 4:1148–1153

    CAS  Google Scholar 

  2. Ghanayem BI, Nyska A, Haseman JK, Bucher JR (2002) Acrylonitrile is a multisite carcinogen in male and female B6C3F1 mice. Toxicol Sci 68:59–68

    Article  CAS  PubMed  Google Scholar 

  3. Singh R, Sharma R, Tewari N, Geetanjali Rawat DS (2006) Nitrilase and its application as a ‘green’ catalyst. Chem Biodivers 3:1279–1287

    Article  CAS  PubMed  Google Scholar 

  4. Nigam VK, Khandelwal AK, Gothwal RK, Mohan MK, Choudhury B, Vidyarthi AS, Ghosh P (2009) Nitrilase catalyzed conversion of acrylonitrile by free and immobilized cells of Streptomyces sp. J Biosci 34:21–26

    Article  CAS  PubMed  Google Scholar 

  5. Mathew CD, NagasawaT Kobayashi M, Yamada H (1998) Nitrilase-catalyzed production of Nicotinnic acid from 3-cyanopyridine in Rhodococcus rhodochrous J1. Appl Environ Microbiol 54:1030–1032

    Google Scholar 

  6. Nagasawa T, Nakamura T, Yamada H (1990) Production acrylic acid and Methacrylic acid using Rhodococcus rhodochrous J1 nitrilase. Appl Microbiol Biotechnol 34:322–324

    CAS  Google Scholar 

  7. Nigam VK, Agarwal A, Sharma M, Ghosh P, Choudhury B (2009) Bioconversion of 3-cyanopyridine to nicotinic acid by a thermostable nitrilase. Res J Biotech 4:32–36

    CAS  Google Scholar 

  8. Agarwal A, Nigam VK (2014) Nitrilase mediated conversion of indole-3-acetonitrile to indole-3-acetic acid. Biocatal Agric Biotechnol 3:351–357

    Google Scholar 

  9. Shen M, Zheng Y, Shen YC (2009) Biosynthesis of p-methoxyphenylacetic acid from p-methoxyphenylacetonitrile by immobilized Bacillus subtilis ZJB-063. Process Biochem 43:978–983

    Google Scholar 

  10. Vega-Hernάndez MC, Leόn-Barrios M, Pèrez-Galdona R (2002) Indole-3-acetic acid production from indole-3-acetonitrile in Bradyrhizobium. Soil Biol Biochem 34:665–668

    Article  Google Scholar 

  11. Wang YJ, Zheng YG, Xue JP, Shen YC (2006) Microbial transformation of indole-3-acetonitrile to indole-3-acetamide by Nocardia sp. 108. Process Biochem 41:1746–1750

    Article  CAS  Google Scholar 

  12. Kobayashi M, Suzuki T, Fujita T, Masuda M, Shimizu S (1995) Occurrence of enzymes involved in biosynthesis of indole-3-acetic acid from indole-3-acetonitrile in plant associated bacteria, Agrobacterium and Rhizobium. Proc Natl Acad Sci USA 92:714–718

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Kobayashsi M, Izui H, Nagasawa T, Yamada H (1993) Nitrilase in biosynthesis of the plant hormone indole-3-acetic acid from indole-3-acetonitrile: cloning of the Alcaligenes gene and site directed mutagenesis of cysteine residues. Proc Natl Acad Sci USA 90:247–251

    Article  Google Scholar 

  14. Kim YB, Yang SJ, Lee JW, Cha J, Hong SY, Chung SH, Lee ST, Rhim SL (1996) Cloning and expression of an amylase gene from Streptomyces albus KSM-35 in Escherichia coli. Food Biotechnol 5:249–253

    Google Scholar 

  15. Voff JN, Altenbuchner J (1998) Genetic instability of the Streptomyces chromomsome. Mol Microbiol 27:239–246

    Article  Google Scholar 

  16. Banga J, Tripathi CKM (2009) Response surface methodology for optimization of medium components in submerged culture of Aspergillus flavus for enhanced heparinise production. Lett Appl Microbiol 49:204–209

    Article  CAS  PubMed  Google Scholar 

  17. Adinarayana K, Ellaiah P (2002) Response surface optimization of the critical medium components for the production of alkaline protease by a new isolated Bacillus sp. J Pharm Pharmaceut Sci 5:272–278

    CAS  Google Scholar 

  18. Dubey S, Singh A, Banerjee UC (2011) Response surface methodology of nitrilase production by recombinant Escherichia coli. Braz J Microbiol 42:1085–1092

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Kumari A, Mahapatra P, Banerjee R (2009) Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogens. Braz Arch Biol Technol 52:1349–1356

    Article  CAS  Google Scholar 

  20. Shen M, Liu ZQ, Zheng YG, Shen YC (2009) Enhancing endonitrilase production by a newly isolated Arthrobacter nitroguajacolicus ZJUTB06-99 through optimization of culture medium. Biotechnol Bioprocess Eng 14:795–802

    Article  CAS  Google Scholar 

  21. Dong HP, Liu ZQ, Zheng YG, Shen YC (2011) Medium optimization for nitrilase production by newly isolated Rhodococcus erythropolis ZJB-0910 using statistical designs. Chem Biochem Eng 25:351–358

    CAS  Google Scholar 

  22. Khandelwal AK, Nigam VK, Choudhury B, Medicherla KM, Ghosh P (2007) Optimization of nitrilase production from a new thermophillic isolate. J Chem Technol Biotechnol 82:642–661

    Google Scholar 

  23. Piotrowski M, Schonfelder S, Weiler EW (2001) The Arabidopsis thaliana isogene NIT4 and its orthologs in Tabacco encode β-cyano-l-alanine hydratase/nitrilase. J Biol Chem 276:2616–2621

    Article  CAS  PubMed  Google Scholar 

  24. Miller GL (1959) Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428

    Article  CAS  Google Scholar 

  25. Standbury PF, Whitaker A, Hall SJ (1998) Princsssiples of fermentation technology. Aditya, New Delhi

    Google Scholar 

  26. Tanyildizi MS, Özer D, Elibol M (2005) Optimization of α-amylase production by Bacillus sp. using response surface methodology. Process Biochem 40:2291–2296

    Article  CAS  Google Scholar 

  27. Kashmiri ZN, Mankar SA (2014) Free radicals and oxidative stress in bacteria. Int J Curr Microbiol App Sci 3:34–40

    CAS  Google Scholar 

  28. Kamal A, Kumar MS, Kumar CG, Shaik T (2011) Bioconversion of acrylonitrile to acrylic acid by Rhodococcus ruber strain AKSH-84. J Microbiol Biotechnol 21:37–42

    Article  CAS  PubMed  Google Scholar 

  29. Yamamoto K, Komatsu K (1991) Purification and characterization of nitrilase responsible for the hydrolysis from Acinetobacter sp. AK 226. Agric Biol Chem 55:1459–1466

    CAS  PubMed  Google Scholar 

  30. Varshney AK, Mohan MK, Vidyarthi AS, Nigam VK, Ghosh P (2013) Statistical optimization of medium components to increase the manganese peroxidise by Phanerochaete chrysoporium NCIM 1197. Biotechnol Bioprocess Eng 18:1176–1184

    Article  CAS  Google Scholar 

  31. Muralidhar RV, Chirumamila RR, Marchant R, Nigam P (2001) A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources. Biochem Eng J 9:17–23

    Article  CAS  Google Scholar 

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Acknowledgments

Authors are thankful to the University Grants Commission, New Delhi, India for providing Junior Research Fellowship to Astha Agarwal (F.2-29/2011; SA-1) for carrying out this research work. Authors are also thankful to Centre of Excellence (COE), Department of Biotechnology under TEQIP phase II (Ref No: NPIU/TEQIP II/FIN/31/158; Dated 16th April 2013) for laboratory facilities and infrastructure support and lastly, authors are grateful to Dr. Alok Varshney for sincere help during experimental work. Authors also declare that there is no financial or commercial conflict of interest.

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  1. Department of Bio-Engineering, Birla Institute of Technology, Mesra, Ranchi, 835 215, India

    Astha Agarwal & Vinod Kumar Nigam

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  1. Astha Agarwal
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  2. Vinod Kumar Nigam
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Correspondence to Vinod Kumar Nigam.

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Agarwal, A., Nigam, V.K. Enhanced Production of Nitrilase from Streptomyces sp. MTCC 7546 by Response Surface Method. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 87, 603–609 (2017). https://doi.org/10.1007/s40011-015-0638-2

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  • DOI: https://doi.org/10.1007/s40011-015-0638-2

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