Abstract
The present work was undertaken to study the effects of 24-epibrassinolide (24-EBL) on percent germination, growth (root length, shoot length, fresh weight and dry weight), lipid peroxidation, sodium and potassium ion concentrations, proline content, total osmolyte content, level of antioxidants (ascorbic acid, tocopherol and glutathione) in 7-day old seedlings of Raphanus sativus exposed to cadmium and mercury toxicity. Results of present study revealed that growth of seedlings was enhanced with the treatment of 24-EBL. In addition, 24-EBL was proved effective to overcome cadmium and mercury stress by altering the level of ions, osmoprotectants and antioxidants of plant.
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Yadav SK (2010) Heavy metals toxicity in plants: an overview on the role of glutathione and phytochelatins in heavy metal stress tolerance of plants. S. Afr J Bot 76:167–179
Kovalchuk O, Titov V, Hohn B (2001) A sensitive transgenic plant system to detect toxic inorganic compounds in the environment. Nat Biotechnol 19(6):568–572
Chen J, Yang ZM (2012) Mercury toxicity, molecular response and tolerance in higher plants. Biometals 25(5):847–857
Israr M, Sahi S, Datta R, Sarkar D (2006) Bioaccumulation and physiological effects of mercury in Sesbania drummonii. Chemosphere 65:591–598
Zhou ZS, Huang SQ, Guo K, Mehta SK, Zhang PC, Yang ZM (2007) Metabolic adaptations to mercury-induced oxidative stress roots of Medicago sativa L. J Inorg Biochem 101:1–9
Seth CS, Remans T, Keunen E, Jozefczak M, Gielen H, Opdenakker K, Weyens N, Vangronsveld J, Cuypers A (2012) Phytoextraction of toxic metals: a central role for glutathione. Plant Cell Environ 35:334–346
Kandelinskaya OL, Topunov AF, Grishchenko ER (2007) Biochemical Aspects of Growth-Stimulating Effects of Steroid Phytohormones on Lupine Plants. Appl Biochem Microbiol 43(3):324–331
Ryu H, Cho H, Kim K, Hwang I (2010) Phosphorylation dependent nucleocytoplasmic shuttling of BES1 is a key regulatory event in brassinosteroid signaling. Mol Cells 29(3):283–290
Khripach VA, Zhabinskii VN, De-Groot AE (2000) Twenty years of brassinosteroids: steroidal plant hormones warrant better crops for the XXI century. Ann Bot 86:441–447
Yu JQ, Huang LF, Hu WH et al (2004) A role for brassinosteroids in regulation of photosynthesis in Cucumis sativus. J Exp Bot 55:1135–1143
Kartal G, Temel A, Arican E, Gozukirmizi N (2009) Effects of brassinosteroids on barley root growth, antioxidant system and cell division. Plant Growth Regul 58:261–267
Benzarti S, Mohri S, Ono Y (2008) Plant response to heavy metal toxicity: comparative study between the hyperaccumulator Thlaspi caerulescens (ecotype Ganges) and nonaccumulator plants: lettuce, radish and alfalfa. Environ Toxicol 23(5):607–616
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Bates LS, Waldren RP, Tear ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
Roe JH, Kuether CA (1943) The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenyl hydrazine derivative of dehydroascorbic acid. J Biol Chem 147:399–407
Martinek RG (1964) Method for the determination of vitamin E (total tocopherols) in serum. Clin Chem 10:1078–1086
Sedlak J, Lindsay RH (1968) Estimation of total, proteinbound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205
Aldoobie NF, Beltagi MS (2013) Physiological, biochemical and molecular responses of common bean (Phaseolus vulgaris L.) plants to heavy metals stress. Afr J Biotechnol 12:4614–4622
Jaleel CA, Jayakumar K, Xing ZC, Azooz MM (2009) Antioxidant potentials protect Vigna radiata (L.) Wilczek plants from soil cobalt stress and improve growth and pigment composition. Plant Omics 2:120–126
Gaur N, Flora G, Yadav M, Tiwari A (2014) A review with recent advancements on bioremediation-based abolition of heavy metals. Environ Sci 16:180–193
Sharma P, Bhardwaj R (2007) Effects of 24-Epibrassinolide on growth and metal uptake in Brassica juncea L. under copper metal stress. Acta Physiol Plant 29:259–263
Choudhary SP, Bhardwaj R, Gupta BD, Dutt P, Gupta RK, Biondi S, Kanwar M (2010) Epibrassinolide induces changes in indole-3-acetic acid, abscisic acid and polyamine concentrations and enhances antioxidant potential of radish seedlings under copper stress. Physiol Plant 140:280–296
Ozdemir F, Bor M, Demiral T, Turkan I (2004) Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regul 42:203–211
Sharma SS, Dietz KJ (2008) The relationship between metal toxicity and cellular redox imbalance. Trends Plant Sci 14:43–49
Alaoui-Sosse B, Genet P, Vinit-Dunand F, Toussaint ML, Epron D, Badot PM (2004) Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant Sci 166:1213–1218
Pietrini F, Iannelli MA, Pasqualini S, Massacci A (2003) Interaction of cadmium with glutathione and photosynthesis in developing leaves and chloroplasts of Phragmites australis (Cav.) Trin. ex Steudel. Plant Physiol 133:829–837
Bajguz A (2000) Blockage of heavy metal accumulation in Chlorella vulgaris cells by 24-epibrassinolide. Plant Physiol Biochem 38:797–801
Choudhary SP, Kanwar M, Bhardwaj R, Yu JQ, Tran LP (2012) Chromium stress mitigation by polyamine-brassinosteroid application involves phytohormonal and physiological strategies in Raphanus sativus L. PLoS ONE 7:e33210
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Authors are also thankful to Department of Botanical and Environmental Sciences, Guru Nanak Dev University- Amritsar (India) for providing laboratory facilities for this work.
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Kapoor, D., Rattan, A., Gautam, V. et al. Alleviation of Cadmium and Mercury Stress by Supplementation of Steroid Hormone to Raphanus sativus Seedlings. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 661–666 (2016). https://doi.org/10.1007/s40011-015-0501-5
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DOI: https://doi.org/10.1007/s40011-015-0501-5