Abstract
Epimorphic regeneration in vertebrates involves the restoration of lost tissue or organs through the formation of a regeneration blastema and occurs through a complex interaction of a number of molecular signaling pathways. Of the many effectors of successful tail regeneration in the lizard Hemidactylus flaviviridis, one crucial pathway is the cyclooxygenase-2 (COX-2) mediated PGE2 signaling pathway. The current study was aimed at understanding whether COX-2 signaling plays any role in the expression of Wnt/β-Catenin signaling components during regenerative outgrowth in H. flaviviridis. Etoricoxib—selective inhibitor of the inducible isoform of COX-2—was administered to lizards orally. We tested the expression of β-Catenin during wound epidermis and blastema stages in the regenerating tail and found a reduction in its expression in response to drug treatment. Further, it was observed that the expression of canonical Wnt ligands was greatly altered due to COX-2 inhibition. Our results provide evidence of a cross-talk between the COX-2 induced PGE2 pathway and Wnt/β-Catenin signaling in the regenerating lizard tail. An understanding of the interaction among various signaling pathways will help elucidate the mechanism underlying epimorphosis in lizards, the only amniotes capable of appendage regeneration.
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Acknowledgements
The authors are thankful to DST-SERB, Govt. of India for financial assistance in the form of Project No. SB/SO/AS-008; CSIR, New Delhi for research fellowship to PB; DBT-MSUB-ILSPARE, The M. S. University of Baroda for providing the LC/MS facility; Adityarao Khnavilkar for his assistance in LC/MS.
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All experimental protocols were approved by the Institutional Animal Ethics Committee (IAEC) constituted as per the guidelines of Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), India (IACUC no. 984/07/2014-2).
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Buch, P.R., Sarkate, P., Uggini, G.K. et al. Inhibition of Cyclooxygenase-2 Alters Wnt/β-Catenin Signaling in the Regenerating Tail of Lizard Hemidactylus flaviviridis . Tissue Eng Regen Med 14, 171–178 (2017). https://doi.org/10.1007/s13770-017-0037-2
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DOI: https://doi.org/10.1007/s13770-017-0037-2