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AtMybL-O modulates abscisic acid biosynthesis to optimize plant growth and ABA signaling in response to drought stress

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Abstract

To combat constant stress from the external environment throughout their life cycle, plants have evolved their own defense mechanisms. Through robust and complicated defense mechanisms, plants have increased their productivity and adaptability under harsh conditions. In this study, we demonstrated the function of the AtMybL-O gene by using knockout (ko) mutants to expand the existing research field. The atmybl-o ko mutant seedlings grew similarly to the wild type (WT) in response to osmotic stress, while the AtMybL-O overexpression lines exhibit growth suppression in the same growth condition. Further, we attempted to understand the functional mechanism of AtMybL-O with respect to stress response toward drought stress. Firstly, we determined the changes in gene expression of the mutants in response to mannitol treatment and identified a strong increase in the expression of COR15b, DREB1A, and NCED3 gene in the mutant. Finally, through abscisic acid (ABA) measurement experiments, we observed that the ABA content of mannitol-treated mutants was higher than that of the WT. Therefore, our results indicate that AtMybL-O modulates ABA biosynthesis and ABA signaling in response to drought stress.

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Acknowledgments

The present study was supported by a Grant from the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (to H.L., 2016; Grant No. 2016-116118-3).

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Authors and Affiliations

  1. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Anam-dong 5-ga, Seongbuk-gu, Seoul, 136-713, Republic of Korea

    Chan Young Jeong, Won Je Lee, Hai An Truong, Cao Sơn Trịnh & Hojoung Lee

  2. Institute of Life Science and Natural Resources, Korea University, Seoul, 136-713, Republic of Korea

    Chan Young Jeong & Hojoung Lee

  3. Department of Molecular Biotechnology, College of Agriculture and Life Sciences, Bioenergy Research Center, Chonnam National University, Gwangju, Republic of Korea

    Suk-Whan Hong

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  1. Chan Young Jeong
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  2. Won Je Lee
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  3. Hai An Truong
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  4. Cao Sơn Trịnh
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  5. Suk-Whan Hong
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Correspondence to Suk-Whan Hong or Hojoung Lee.

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Jeong, C.Y., Lee, W.J., Truong, H.A. et al. AtMybL-O modulates abscisic acid biosynthesis to optimize plant growth and ABA signaling in response to drought stress. Appl Biol Chem 61, 473–477 (2018). https://doi.org/10.1007/s13765-018-0376-2

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  • DOI: https://doi.org/10.1007/s13765-018-0376-2

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