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Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction

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Abstract

The efficiency to remove polycyclic aromatic hydrocarbons (PAHs) was determined using a hemoglobin-catalyzed biocatalytic reaction. The present study employed pyrene as a model of PAHs to study its oxidative removal in the presence of H2O2 and hemoglobin in mass ratio of 3:1. The extent of pyrene removal reached up to 91.1% in the presence of H2O2 and hemoglobin. However, the extent of pyrene removal was 21.3% in the presence of H2O2 only. The results indicate that pyrene removal might be due to pyrene oxidation by the biocatalytic reaction. Overall, this study demonstrated that hemoglobin-catalyzed biocatalytic reactions could remediate pyrene effectively.

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Acknowledgment

This study was supported by the Korea Basic Science Institute (KBSI) and was partially supported by a grant from Hankuk University of Foreign Studies.

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Authors and Affiliations

  1. Department of Environmental Science, Hankuk University of Foreign Studies, Youngin-si, Gyeonggi-do, 17035, Republic of Korea

    Haein Keum & Guyoung Kang

  2. Department of Biosystems Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea

    Namhyun Chung

Authors
  1. Haein Keum
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  2. Guyoung Kang
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  3. Namhyun Chung
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Correspondence to Guyoung Kang or Namhyun Chung.

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Keum, H., Kang, G. & Chung, N. Oxidation of pyrene using a hemoglobin-catalyzed biocatalytic reaction. Appl Biol Chem 60, 505–508 (2017). https://doi.org/10.1007/s13765-017-0305-9

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  • DOI: https://doi.org/10.1007/s13765-017-0305-9

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