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Evaluation of the anti-osteoporosis and antioxidant activities of phenolic compounds from Euphorbia maculata

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Abstract

Antioxidant and anti-osteoporosis activities of extracts and chemical constituents from the whole plant of Euphorbia maculata were investigated. The MeOH extract, as well as EtOAc and H2O fractions (10.0 μg/mL), exhibited potent antioxidant activities. Their oxygen radical absorbance capacity and cupric ion reducing antioxidant capacity values were 27.07±0.31 to 28.47±0.36 and 43.86±0.26 to 46.67±0.34 fold higher than those of 1.0 μM Trolox, respectively. The MeOH extract and EtOAc fraction (at 10.0 μg/mL) also significantly suppressed excessive bone resorption by osteoclasts with tartrate-resistant acid phosphatase (TRAP) activity values of 154.90±4.25 and 163.95±9.77%, respectively. Bioassay guided isolation of the EtOAc and H2O fractions afforded 19 known compounds (1–19). Of these, compounds 18, and 13–15 showed good antioxidant activity based on peroxyl radical-scavenging and reducing capacity assays, whereas compounds 1, 4, 7, and 14 showed the most significant inhibitory effect with TRAP activity values ranging from 121.31±1.41 to 110.00±3.74% relative to the control.

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Authors and Affiliations

  1. College of Pharmacy, Chungnam National University, Daejeon, 305-764, Republic of Korea

    Bui Thi Thuy Luyen, Bui Huu Tai, Nguyen Phuong Thao & Young Ho Kim

  2. Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam

    Bui Huu Tai & Nguyen Phuong Thao

  3. Department of Food and Nutrition, Hannam University, Daejeon, 305-811, Republic of Korea

    Sang Hyun Lee & Hae Dong Jang

  4. Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang Oriental Medicines Research Institute, Iksan, Jeonbuk, 570-749, Republic of Korea

    Young Mi Lee

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  1. Bui Thi Thuy Luyen
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  2. Bui Huu Tai
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  3. Nguyen Phuong Thao
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  4. Sang Hyun Lee
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Correspondence to Hae Dong Jang or Young Ho Kim.

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Luyen, B.T.T., Tai, B.H., Thao, N.P. et al. Evaluation of the anti-osteoporosis and antioxidant activities of phenolic compounds from Euphorbia maculata . J Korean Soc Appl Biol Chem 57, 573–579 (2014). https://doi.org/10.1007/s13765-014-4157-2

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  • DOI: https://doi.org/10.1007/s13765-014-4157-2

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