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Determination of 5′-mononucleotides in infant formula by capillary electrophoresis with ultraviolet detection

紫外检测-毛细管电泳法测定婴儿奶粉中的 5单磷酸核苷酸

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Dairy Science & Technology

Abstract

A method for the simultaneous determination of five 5′-mononucleotides in infant formula by capillary electrophoresis (CE) with ultraviolet detection was developed. The analysis was performed using a bare fused-silica capillary (75 μm, i.d. × 50.2 cm; effective length, 40 cm) with a separation buffer consisting of 0.03 mol.L−1 sodium tetraborate and 0.08 mol.L−1 hydroxypropyl-β-cyclodextrin at pH 10.00. The samples were deproteinized with 0.05 mol.L−1 acetic acid. Limits of detection were between 0.8 and 1.0 mg.L−1 with an injection volume of 96 nL. The precision of the method was in the range of between 2.0% and 5.2% (n = 7). The recoveries at the concentrations studied (5 and 20 mg.L−1) were between 87.3% and 113.5%. The method was used for the analysis of five different kinds of infant formula, and the results were compared with the HPLC method. The CE method, which had high separation efficiency, consumed no solvent and could be used as an alternative to the HPLC method.

紫外检测-毛细管电泳法测定婴儿奶粉中的 5单磷酸核苷酸

摘要 : 本文建立了紫外检测-毛细管电泳 (CE-UV) 测定婴儿配方奶粉中5′-单磷酸核苷酸的新方法。以未涂覆石英毛细管柱(50.2 cm × 75 μm, 有效长度40 cm)为分离柱、以0.03 mol.L−1 四硼酸钠及0.08 mol.L−1 羟丙基β-环糊精为分离缓冲液 (pH 10.0),于254 nm 波长处进行紫外检测。样品前处理简单,仅需0.05 mol.L−1 醋酸沉淀蛋白。方法检出限在0.8 ~ 1 mg.L−1 (进样体积96 nL)间。方法精密度在2.0% ~ 5.2% (n = 7)间。在测定的浓度范围内(5 mg.L−1 and 20 mg.L−1)回收率在87.3% ~ 113.5% 间。用该法分析了5种婴儿配方奶粉,并与文献报道的高效液相色谱法 (HPLC) 测定的结果进行了比较。结果表明:CE法无需有机溶剂且分离效率高,可以成为HPLC法的替代方法.

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Authors and Affiliations

  1. Beijing Center for Disease Control and Prevention, Beijing, 100013, China

    Xiaojing Ding, Yun Li & Shan Zhao

  2. School of Public Health and Family Medicine, Capital Medical University, Beijing, 100069, China

    Xiaojing Ding & Yun Li

  3. College of Food Science and Technology, Agricultural University of Hebei, Baoding, 071001, China

    Baohua Song & Zhi Wang

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  1. Xiaojing Ding
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  2. Yun Li
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  3. Baohua Song
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  4. Shan Zhao
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  5. Zhi Wang
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Correspondence to Xiaojing Ding.

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Ding, X., Li, Y., Song, B. et al. Determination of 5′-mononucleotides in infant formula by capillary electrophoresis with ultraviolet detection. Dairy Science & Technol. 91, 759–770 (2011). https://doi.org/10.1007/s13594-011-0035-4

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  • DOI: https://doi.org/10.1007/s13594-011-0035-4

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