Uncommon selection by root system size increases barley yield

Abstract

Most agronomy practices such as fertilization, irrigation, and soil treatment involve plant root interactions. However, the role of plant roots is rarely assessed during agricultural experiments due to the lack of suitable methods. Plant varieties with a larger root system use soil water and nutrients in dry environments more effectively than varieties with a smaller root system. Such large root varieties can be developed by breeding. Therefore, we evaluated the effects of selection for large or small root systems in 12 barley populations developed via the mutual crossing of four parents in the F₃ generation as a response to selection in the preceding F₂ generation. Root system size was analyzed by measuring electrical capacitance. Results show that our selection method was effective because the progeny of plants with larger root systems also had larger root systems. Conversely, the progeny of plants with smaller root systems also had smaller root systems. The average differences were +40 and −43 % in the parental segregating generation and +4 and −2 % in the progeny. The root system size impacted the grain yield, which showed a twofold greater response to selection. Indeed, plants with a root system increase of 3.9 % exhibited a yield increase of 8.1 %. Some varieties transmitted larger root systems to their progeny, and some transmitted smaller root systems. Thus, we show that the method used enabled the comparison of root system size in the same crop, in the same soil, and at the same time, which is particularly suitable for selecting root system size in practical breeding. We do not know any other suitable method for the repeated evaluation of intact plants and harvesting of seeds from the selected plants. Root system size has not yet been reported by other authors as a selection criterion in practical breeding, with the exception of root crops such as sugar beet and carrots.