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In vitro evaluation of sugar syrups, antibiotics, and miticides on growth of honey bee pathogen, Ascosphaera apis: Emphasis for chalkbrood prevention is on keeping bees healthy

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Abstract

To examine the link between chalkbrood and treatments applied to honey bee (Apis mellifera) colonies, growth of Ascosphaera apis was determined under chalkbrood-inducing conditions on agar containing crushed bee larvae as a mock infection. Treatments were tested at concentrations in the field, representative of beekeeping practice (at least in the USA). A chilling or warming stress known to heighten chalkbrood caused no growth enhancement of A. apis. Miticides had a negative effect; formic acid killed A. apis and oxalic acid reduced growth by 70 %. High-fructose corn syrups and sucrose syrup undiluted, a 50:50 mixture, alone, and with antibiotics did not alter growth of A. apis. We conclude that chalkbrood does not develop from a temperature- or treatment-induced activation of A. apis. Sugar syrups, miticides, and antibiotics point out other health problems that make bees prone to infection by A. apis. Thus, chalkbrood opportunistically infects bees under other forms of stress.

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REFERENCES

  • Agner, G., Gyórbiró, A., Valkó, I., Vasvári-Debreczy, L., Szabó, A., Farkas, G., Halász, J., Podányi, B., Brlik, J., Simon, K., Hermecz, I. (2003) Light- and temperature-induced degradation of fumagillin. Acta Pharm. Hung. 73, 41–45

    PubMed  Google Scholar 

  • Aliano, N.P., Ellis, M.D., Siegfried, B.D. (2006) Acute contact toxicity of oxalic acid to Varroa destructor (Acari: Varroidae) and their Apis mellifera (Hymenoptera: Apidae) hosts in laboratory bioassays. J. Econ. Entomol. 99, 1579–1582

    Article  CAS  PubMed  Google Scholar 

  • Amrine Jr., J.W., Noel, R.C., Webb, D. (2007) Results of 50 % formic acid fumigation of honey bee hives [Apis mellifera ligustica (Hymenoptera: Apidae)] to control varroa mites (Acari: Varroidae) in brood combs in Florida, U.S.A. Int. J. Acarol. 33, 99–109

    Article  Google Scholar 

  • Aronstein, K.A., Cabanillas, H.E. (2012) Chalkbrood re-examined. In: Sammataro, D., Yoder, J.A. (eds.) Honey Bee Colony Health: Challenges and Sustainable Solutions, pp. 121–130. CRC Press, Boca Raton

    Google Scholar 

  • Baldrian, P., Gabriel, J. (2002) Intraspecific variability in growth response to cadmium of the wood-rotting fungus Piptoporus betulinus. Mycologia 94, 428–436

    Article  CAS  PubMed  Google Scholar 

  • Chiesa, F., Milani, N., D’Agaro, M. (1989) Observations of the reproductive behavior of Varroa jacobsoni Oud.: techniques and preliminary results. In: Cavalloro, R. (ed.) Proceeding of the Meeting of the EC-Experts’ Group, Udine 1988, pp. 213–222. CEC, Luxembourg

    Google Scholar 

  • Christensen, M., Gilliam, M. (1983) Notes on the Ascosphaera species inciting chalkbrood in honey bees. Apidologie 14, 291–297

    Article  Google Scholar 

  • Cooper, B. (1980) Fluctuating broodnest temperature rhythm. British Isles Bee Breeders News 18, 12–16

    Google Scholar 

  • de Miranda, J.R., Gauthier, L., Ribière, M., Chen, Y.P. (2012) Honey bee viruses and their effect on bee and colony health. In: Sammataro, D., Yoder, J.A. (eds.) Honey Bee Colony Health: Challenges and Sustainable Solutions, pp. 71–102. CRC Press, Boca Raton

    Google Scholar 

  • Flores, J.M., Ruiz, J.A., Ruz, J.M., Puert, F., Bustos, M., Padilla, F., Campano, F. (1996) Effect of temperature and humidity of sealed brood on chalkbrood development under controlled conditions. Apidologie 27, 185–192

    Article  Google Scholar 

  • Gilliam, M. (1986) Infectivity and survival of the chalkbrood pathogen. Ascosphaera apis, in colonies of honey bees, Apis mellifera, Apidologie 17, 93–100

    Google Scholar 

  • Gilliam, M., Taber III, S., Lorenz, B.J., Prest, D.B. (1988) Factors affecting development of chalkbrood disease in colonies of honey bees, Apis mellifera, fed pollen contaminated with Ascosphaera apis. J. Invertebr. Pathol. 52, 314–325

    Article  Google Scholar 

  • Hitchcock, J.D., Moffett, J.O., Lackett, J.J., Elliot, J.R. (1970) Tylosin for control of American foulbrood disease in honey bees. J. Econ. Entomol. 63, 204–207

    CAS  Google Scholar 

  • Huang, W.-F., Solter, L.F., Yau, P.M., Imai, B.S. (2013) Nosema ceranae escapes fumagillin control in honey bees. Plos Pathogens 9(3), e1003185. doi:10.1371/journal.ppat.1003185

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jennings, D.H., Lysek, G. (1999) Fungal Biology: Understanding the Fungal Lifestyle. Springer, New York

    Google Scholar 

  • Jimenez, M.I., Aguar, M.P., Olmo, V., Galiano, A., Martinez Fernandez, A.R. (1994) In vitro antifungal activity of IQB-863-F, amphotericin B and other imidazole derivatives in front of Ascosphaera apis. Rev. lberoam. Micol. 11, 7–9

    Google Scholar 

  • Johnson, R.N., Zaman, M.T., Decelle, M.M., Siefel, A.J., Tarpy, D.R., Siegel, E.C., Starks, P.T. (2005) Multiple micro-organisms in chalkbrood mummies: evidence and implications. J. Apic. Res. 44, 29–32

    Google Scholar 

  • Katznelson, H., Jamieson, C.A. (1952) Control of Nosema disease of honeybees with fumagillin. Science 115, 70–71

    Article  CAS  PubMed  Google Scholar 

  • Mao, W., Schuler, M.A., Berenbaum, M.R. (2013) Honey constituents up-regulate detoxification and immunity genes in the western honey bee Apis mellifera. Proc. Nat. Acad. Sci. 110, 8842–8846

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mudroňová, D., Toporčák, J., Nemcová, R., Gancarčíková, S., Hajdučková, V., Rumanovská, K. (2011) Lactobacillus sp. as a potential probiotic for the prevention of Paenibacillus larvae infection in honey bees. J. Apic. Res. 50, 323–324

    Article  Google Scholar 

  • Peng, C.Y.-S., Mussen, E., Fong, A., Cheng, P., Wong, G., Montague, M.A. (1996) Laboratory and field studies on the effects of the antibiotic tylosin on honey bee Apis mellifera L. (Hymenoptera: Apidae) development and prevention of America foulbrood disease. J. Invertebr. Pathol. 67, 65–71

    Article  CAS  PubMed  Google Scholar 

  • Rademacher, E., Harz, M. (2006) Oxalic acid for the control of varroosis in honey bee colonies - review. Apidologie 37, 98–120

    Article  CAS  Google Scholar 

  • Sahinler, N., Kurt, S. (2004) A study on antifungal activity of formic acid and propolis extract against chalkbrood disease pathogen Ascosphaera apis. J. Anim. Vet. Adv. 3, 554–556

    Google Scholar 

  • Sammataro, D. (2012) Global status of honey bee mites. In: Sammataro, D., Yoder, J.A. (eds.) Honey Bee Colony Health: Challenges and Sustainable Solutions, pp. 37–54. CRC Press, Boca Raton

    Google Scholar 

  • Sokal, R.R., Rohlf, F.J. (1995) Biometry: The Principles and Practice of Statistics in Biological Research. Freeman, San Francisco

    Google Scholar 

  • Southwick, E.E., Heldmaier, G. (1987) Temperature control in honey bee colonies. BioScience 37, 395–399

    Article  Google Scholar 

  • Underwood, R.M., Currie, R.W. (2005) Effect of concentration and exposure time on treatment efficacy against varroa mites (Acari: Varroidae) during indoor winter fumigation on honey bees (Hymenoptera: Apidae) with formic acid. J. Econ. Entomol. 98, 1802–1809

    Article  CAS  PubMed  Google Scholar 

  • Winston, P.W., Bates, D.S. (1960) Saturated solutions for the control of humidity in biological research. Ecology 41, 232–237

    Article  Google Scholar 

  • Yamaji, K., Ishimoto, H., Usui, N., Mori, S. (2005) Organic acid and water-soluble phenolics produced by Paxillus sp. 60/92 together show antifungal activity against Pythium vexans under acidic culture conditions. Mycorrhiza 15, 17–23

    Article  CAS  PubMed  Google Scholar 

  • Yoder, J.A., Christensen, B.S., Croxall, T.J., Tank, J.L., Sammataro, D. (2008) Suppression of growth rate of colony-associated fungi by high fructose corn syrup feeding supplement, formic acid, and oxalic acid. J. Apic. Res. 47, 126–130

    Article  CAS  Google Scholar 

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ACKNOWLEDGMENTS

We thank Diana Sammataro (Carl Hayden Honey Bee Research Center, Tucson, Arizona) for her helpful comments on an earlier draft of this article. Support for this project was made possible by a Wittenberg University Research Grant to AJJ and CLM from funding provided by James R. Wymer.

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Authors and Affiliations

  1. Department of Biology, Wittenberg University, Springfield, OH, 45501, USA

    Jay A. Yoder, Andrew J. Jajack, William S. Cornacchione, Allison L. Dunn, Edward G. Cunningham, Caroline L. Matchett & Andrew E. Rosselot

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  1. Jay A. Yoder
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  2. Andrew J. Jajack
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  3. William S. Cornacchione
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  4. Allison L. Dunn
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  5. Edward G. Cunningham
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  6. Caroline L. Matchett
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  7. Andrew E. Rosselot
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Correspondence to Jay A. Yoder.

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Manuscript editor: Peter Rosenkranz

Evaluation in vitro de solutions sucrées, d’antibiotiques et d’acaricides sur le développement du pathogène d’abeilles, Ascosphaera apis : l’important est de maintenir les abeilles en bonne santé pour prévenir la maladie du couvain plâtré

Colonie d’abeilles / mycose / acide formique / acide oxalique / sirop de maïs à haute teneur en fructose

In vitro Analyse von Zuckersyrup, Antibiotika und Akariziden auf das Wachstum des Honigbienenpathogens Ascophaera apis : Kalkbrutvorbeugung bedeutet, die Bienen gesund zu halten

Bienenvolk / Pilzinfektion / Ameisensäure / Oxalsäure / Fruktose-Maissyrup / Antibiotika

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Yoder, J.A., Jajack, A.J., Cornacchione, W.S. et al. In vitro evaluation of sugar syrups, antibiotics, and miticides on growth of honey bee pathogen, Ascosphaera apis: Emphasis for chalkbrood prevention is on keeping bees healthy. Apidologie 45, 568–578 (2014). https://doi.org/10.1007/s13592-014-0274-5

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  • DOI: https://doi.org/10.1007/s13592-014-0274-5

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