Abstract
In this study, the role of hydrogen peroxide (H2O2), acting as a ROS, on physical and biochemical properties of tomatoes was investigated. Tomato fruits at slight breaker stage were immersed in a series of solutions of H2O2 (0, 10, 100, and 500 mM) for 30 min and then stored at room temperature for 4 weeks. The 100 mM H2O2 treatment significantly increased fruit firmness and decreased water-soluble pectin and expression of cell wall related genes, polygalacturonase (SlPG) and pectate lyase (SlPL). There was no significant change in ethylene and respiration rates between any conditions. Moreover, increasing H2O2 concentration decreased proline content, and the lowest proline level was found in tomato fruits treated with 100 mM H2O2. These results suggested that the overall morphological and biochemical quality of tomato could be effectively maintained by 100 mM H2O2 treatment in postharvest storage conditions.
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Abdel Latef AAA, Kordrostami M, Zakir A, Zaki H, Saleh OM (2019) Eustress with H2O2 facilitates plant growth by improving tolerance to salt stress in two wheat cultivars. Plants 8:303. https://doi.org/10.3390/plants8090303
Abdeldym EA, El-Mogy MM, Abdellateaf HRL, Atia MAM (2020) Genetic characterization, agro-morphological and physiological evaluation of grafted tomato under salinity stress conditions. Agronomy 10:1948. https://doi.org/10.3390/agronomy10121948
AL-Saikhan MS, Shalaby TA (2019) Effect of hydrogen peroxide (H2O2) treatment on physicochemical characteristics of tomato fruits during postharvest storage. Aust J Crop Sci 13:798–802. https://doi.org/10.21475/ajcs.19.13.05.p1738
Antoniou C, Chatzimichail G, Xenofontos R, Pavlou JJ, Panagiotou E, Christou A, Fotopoulos V (2017) Melatonin systemically ameliorates drought stress-induced damage in Medicago sativa plants by modulating nitro-oxidative homeostasis and proline metabolism. J Pineal Res 62:12401. https://doi.org/10.1111/jpi.12401
Bayoumi YA (2008) Improvement of postharvest keeping quality of white pepper fruits (Capsicum annuum, L.) by hydrogen peroxide treatment under storage conditions. Acta Biol Szeged 52:7–15
Billy L, Mehinagic E, Royer G, Renard CMGC, Arvineset G, Prost C, Jourjon F (2008) Relationship between texture and pectin composition of two apple cultivars during storage. Postharvest Biol Technol 47:315–324. https://doi.org/10.1016/j.postharvbio.2007.07.011
Brummel DA (2006) Cell wall disassembly in ripening fruit. Funct Plant Biol 33:103–119. https://doi.org/10.1071/FP05234
Brummel DA, Harpster MH (2001) Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants. Plant Mol Biol 47:311–339. https://doi.org/10.1023/A:1010656104304
Čamagajevac I, Maronić D, Pfeiffer T, Bek N, Lončarić Z (2019) Nitric oxide and hydrogen peroxide in plant response to biotic stress. In: Gupta D, Palma J, Corpas F (eds) Nitric oxide and hydrogen peroxide signaling in higher plants. Springer, Cham. https://doi.org/10.1007/978-3-030-11129-8_11
Chen YH, Hung YC, Chen MY, Lin MS, Lin HT (2019) Enhanced storability of blueberries by acidic electrolyzed oxidizing water application may be mediated by regulating ROS metabolism. Food Chem 270:229–235. https://doi.org/10.1016/j.foodchem.2018.07.095
Chen T, Qin G, Tian S (2020) Regulatory network of fruit ripening: current understanding and future challenges. New Phytologist 228:1219–1226. https://doi.org/10.1111/nph.16822
Divis P, Smilek J, Porizka J, Stursa V (2018) The quality of ketchups from the Czech Republic’s market in terms of their physico-chemical properties. Potravinarstvo Slovak J Food Sci 12:233–240
FAO (Food and Agriculture Organization of the United Nations) (2021) Dataset. http://www.fao.org/faostat/en/#data/TCL. Accessed Sept 2021
Felizini E, Lichter A, Smilanik JL, Ippolito A (2016) Disinfecting agents for controlling fruit and vegetable diseases after harvest. Postharvest Biol Technol 122:53–69
Figueroa CR, Rosli HG, Civello PM, Martínez GA, Herrera R, Moya-León MA (2010) Changes in cell wall polysaccharides and cell wall degrading enzymes during ripening of Fragaria chiloensis and Fragaria ×ananassa fruits. Sci Hortic 124:454–462
Filisetti-Cozzi TMCC, Carpita NC (1991) Measurement of uronic acids without interference from neutral sugars. Anal Biochem 197:157–162. https://doi.org/10.1016/0003-2697(91)90372-z
Foyer CH (2018) Reactive oxygen species, oxidative signaling and the regulation of photosynthesis. Environ Exp Bot 154:134–142. https://doi.org/10.1016/j.envexpbot.2018.05.003
Gao H, Zhang Z, Lv XG, Cheng N, Peng BZ, Cao W (2016) Effect of 24-epibrassinolide on chilling injury of peach fruit in relation to phenolic and proline metabolisms. Postharvest Biol Technol 111:390–397. https://doi.org/10.1016/j.postharvbio.2015.07.031
Giuffrè AM, Zappia C, Capocasale M (2017) Tomato seed oil for edible use: cold break, hot break and harvest year effects. J Food Process Preserve 41:e13309. https://doi.org/10.1111/jfpp.13309
Guo DL, Wang ZG, Li Q, Gu SC, Zhang GH, Yu YH (2019) Hydrogen peroxide treatment promotes early ripening of Kyoho grape. Aust J Grape Wine Res 25:357–362. https://doi.org/10.1111/ajgw.12399
Guo DL, Wang ZG, Pei MS et al (2020) Transcriptome analysis reveals mechanism of early ripening in Kyoho grape with hydrogen peroxide treatment. BMC Genomics 21:784. https://doi.org/10.1186/s12864-020-07180-y
Herrera-Vásquez A, Salinas P, Holuigue L (2015) Salicylic acid and reactive oxygen species interplay in the transcriptional control of defense genes expression. Front Plant Sci 6:171
Ji Y, Hu W, Liao J, Xiu Z, Jiang Z, Guan Y, Yang X, Feng K (2021) Ethanol vapor delays softening of postharvest blueberry by retarding cell wall degradation during cold storage and shelf life. Postharvest Biol Technol 177:111538. https://doi.org/10.1016/j.postharvbio.2021.111538
Jiménez A, Creissen G, Kular B, Firmin J, Robinson S, Verhoeyen M, Mullineaux P (2002) Changes in oxidative processes and components of the antioxidant system during tomato fruit ripening. Planta 214:751–758. https://doi.org/10.1007/s004250100667
Jones JB, Jones JP, Stall RE, Zitter TA (1991) Infectious diseases: diseases caused by fungi. Compendium of Tomato Diseases. The American Phytopathological Society, St. Paul, pp 9–25
Junglee S, Urban L, Sallanon H, Lopez-Lauri F (2014) Optimized assay for hydrogen peroxide determination in plant tissue using potassium iodide. Am J Analyt Chem 5:511081. https://doi.org/10.4236/ajac.2014.511081
Klee HJ, Giovannoni JJ (2011) Genetics and control of tomato fruit ripening and quality attributes. Annu Rev Genet 45:41–59
Koltun SJ, MacIntosh AJ, Goodrich-Schnider RM, Klee HJ, Hutton SF, Sarnoski PJ (2021) Sensory and chemical characteristics of tomato juice from fresh market cultivars with comparison to commercial tomato juice. Flavour Frag J 36:121–136
Kumar V, Irfan M, Ghosh S, Chakraborty N, Chakraborty S, Datta A (2016) Fruit ripening mutants reveal cell metabolism and redox state during ripening. Protoplasma 253:581–594. https://doi.org/10.1007/s00709-015-0836-z
Kumar N, Tokas J, Raghavendra M, Singal HR (2020) Impact of exogenous salicylic acid treatment on the cell wall metabolism and ripening process in postharvest tomato fruit stored at ambient temperature. Int J Food Sci 56:2961–2972. https://doi.org/10.1111/ijfs.14936
Lacan D, Baccou JC (1998) High levels of antioxidant enzymes correlate with delayed senescence in nonnetted muskmelon fruits. Planta 204:377–382
Liao WB, Zhang ML, Huang GB, Yu JH (2012) Hydrogen peroxide in the vase solution increases vase life and keeping quality of cut Orienta l × Trumpet hybrid lily ‘Manissa’. Sci Hort 139:32–38
Lin Y, Lin Y, Lin H, Lin M, Li H, Yuan F, Chen Y, Xiao J (2018) Effects of paper containing 1-MCP postharvest treatment on the disassembly of cell wall polysaccharides and softening in Younai plum fruit during storage. Food Chem 264:1–8. https://doi.org/10.1016/j.foodchem.2018.05.031
Lin YX, Lin HT, Chen YH, Wang H, Lin MS, Ritenour MA et al (2020) The role of ROS-induced change of respiratory metabolism in pulp breakdown development of longan fruit during storage. Food Chem 305:125439. https://doi.org/10.1016/j.foodchem.2019.125439
Lin Y, Lin H, Wang H, Lin M, Chen Y, Fan Z, Hung YC, Lin Y (2020) Effects of hydrogen peroxide treatment on pulp breakdown, softening, and cell wall polysaccharide metabolism in fresh longan fruit. Carbohydr Polym 242:116427. https://doi.org/10.1016/j.carbpol.2020.116427
Liu T, Ye X, Li M, Li J, Qi H, Hu X (2020) H2O2 and NO are involved in trehalose-regulated oxidative stress tolerance in cold-stressed tomato plants. Environ Exp Bot 171:103961. https://doi.org/10.1016/j.envexpbot.2019.103961
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262
Lunn D, Phan TD, Tucker GA, Lycett GW (2013) Cell wall composition of tomato fruit changes during development and inhibition of vesicle trafficking is associated with reduced pectin levels and reduced softening. Plant Physiol Biochem 66:91–97. https://doi.org/10.1016/j.plaphy.2013.02.005
Mansourbahmani S, Ghareyazie B, Zarinnia V, Kalatejari S, Mohammadi RZ (2018) Study on the efficiency of ethylene scavengers on the maintenance of postharvest quality of tomato fruit. J Food Meas Charact 12:691–701. https://doi.org/10.1007/s11694-017-9682-3
Marinho HS, Real C, Cyrne L, Soares H, Antunes F (2014) Hydrogen peroxide sensing, signalling and regulation of transcription factors. Redox Biol 2:535–562. https://doi.org/10.1016/j.redox.2014.02.006
Martí R, Roselló S, Cebolla-Cornejo J (2016) Tomato as a source of carotenoids and polyphenols targeted to cancer prevention. Cancers 8:58. https://doi.org/10.3390/cancers8060058
Meegahawaththa WK, Singhalage ID, Mudannayake DC (2020) Tomato (Lycopersicon esculentum L.) peel powder as a source of natural antioxidant and a colorant in stirred yoghurt. Food Life 2:87–97. https://doi.org/10.5851/fl.2020.e10
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410. https://doi.org/10.1016/s1360-1385(02)02312-9
Mohammadrezakhani S, Hajilou J, Rezanejad F, Nahandi FZ (2019) Assessment of exogenous application of proline on antioxidant compounds in three Citrus species under low temperature stress. J Plant Interact 14:347–358. https://doi.org/10.1080/17429145.2019.1629033
Nangare DD, Singh Y, Kumar PS, Minhas PS (2016) Growth, fruit yield and quality of tomato (Lycopersicon esculentum Mill.) as affected by deficit irrigation regulated on phenological basis. Agric Water Manag 171:73–79
Ning Zhang X, Liao YW, Wang XR, Zhang L, Ahammed GJ, Li X, Li QY (2020) Epigallocatechin-3-gallate enhances tomato resistance to tobacco mosaic virus by modulating RBOH1-dependent H2O2 signalling. Plant Physiol Biochem 150:263–269. https://doi.org/10.1016/j.plaphy.2020.03.008
Pattyn J, Vaughan-Hirsch J, de Poel BV (2020) The regulation of ethylene biosynthesis: a complex multilevel control circuitry. New Phytol 229:770–782. https://doi.org/10.1111/nph.16873
Pech JC, Purgatto E, Bouzayen M, Latche A (2012) Ethylene and fruit ripening. Annu Plant Rev 44:275–304. https://doi.org/10.1002/9781118223086.ch11
Pinheiro J, Ganhão R, Gonçalves EM, Silva CLM (2019) Assessment of thermosonication as postharvest treatment applied on whole tomato fruits: optimization and validation. Foods 8:649. https://doi.org/10.3390/foods8120649
Pokalsky AR, Hiatt WR, Ridge N, Rasmussen R, Houck CM, Shewmaker CK (1989) Structure and expression of elongation factor 1α in tomato. Nucleic Acids Res 17:466–4673
Qin G, Meng X, Wang Q, Tian S (2009) Oxidative damage of mitochondrial proteins contributes to fruit senescence: a redox proteomics analysis. J Proteome Res 8:2449–2462. https://doi.org/10.1021/pr801046m
Ren Y, He J, Liu H, Liu G, Ren X (2016) Nitric oxide alleviates deterioration and preserves antioxidant properties in ‘tainong’ mango fruit during ripening. Hortic Environ Biotechnol 58:27–37. https://doi.org/10.1007/s13580-017-0001-z
Ribeiro CW, Korbes AP, Garighan JA, Jardim-Messeder D, Carvalho FEL, Sousa RHV et al (2017) Rice peroxisomal ascorbate peroxidase knockdown affects ROS signaling and triggers early leaf senescence. Plant Sci 263:55–65. https://doi.org/10.1016/j.plantsci.2017.07.009
Wakabayashi K, Chun JP, Huber DJ (2000) Extensive solubilization and depolymerization of cell wall polysaccharides during avocado (Persea americana) ripening involves concerted action of polygalacturonase and pectinmethylesterase. Physiol Plant 108:345–352. https://doi.org/10.1034/j655.1399-3054.2000.t01-1-100402.x
Wang Y, Luo Z, Mao L, Ying T (2016) Contribution of polyamines metabolism and GABA shunt to chilling tolerance induced by nitric oxide in cold-stored banana fruit. Food Chem 197:333–339. https://doi.org/10.1016/j.foodchem.2015.10.118
Wang D, Yeats TH, Uluisik S, Rose JKC, Seymour GB (2018) Fruit softening: revisiting the role of pectin. Trends Plant Sci 23:302–310. https://doi.org/10.1016/j.tplants.2018.01.006
Wang D, Samsulrizal NH, Yan C, Allcock NS, Craigon J, Blanco-Ulate B, Ortega-Salazar I, Marcus SE, Bagheri HM, Fons LP, Fraser PD, Foster T, Fray R, Knox JP, Seymour GB (2019) Characterization of CRISPR mutants targeting genes modulating pectin degradation in ripening tomato. Plant Physiol 179:544–555. https://doi.org/10.1104/pp.18.01187
Wang H, Chen Y, Lin H, Lin M, Chen Y, Lin Y (2020) 1-Methylcyclopropene containing-papers suppress the disassembly of cell wall polysaccharides in Anxi persimmon fruit during storage. Int J Biol Macromol 151:723–729. https://doi.org/10.1016/j.ijbiomac.2020.02.146
Waszczak C, Kerchev PI, Muhlenbock P, Hoeberichts FA, Van Der Kelen K, Mhamdi A et al (2016) SHORT-ROOT deficiency alleviates the cell death phenotype of the Arabidopsis catalase2 mutant under photorespiration-promoting conditions. Plant Cell 28:1844–1859. https://doi.org/10.1105/tpc.16.00038
Wei C, Ma L, Cheng Y, Guan Y, Guan J (2019) Exogenous ethylene alleviates chilling injury of ‘Huangguan’ pear by enhancing the proline content and antioxidant activity. Sci Hortic 257:208671. https://doi.org/10.1016/j.scienta.2019.108671
Zhao Y, Zhu X, Hou Y, Wang X, Li X (2019) Effects of nitric oxide fumigation treatment on retarding cell wall degradation and delaying softening of winter jujube (Ziziphus jujuba Mill. cv. Dongzao) fruit during storage. Postharvest Biol Technol 156:110954. https://doi.org/10.1016/j.postharvbio.2019.110954
Zhong TY, Yao GF, Wang SS (2021) Hydrogen sulfide maintains good nutrition and delays postharvest senescence in postharvest tomato fruits by regulating antioxidative metabolism. J Plant Growth Regul. https://doi.org/10.1007/s00344-021-10377-4
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Torun, H., Uluisik, S. Postharvest application of hydrogen peroxide affects physicochemical characteristics of tomato fruits during storage. Hortic. Environ. Biotechnol. 63, 391–401 (2022). https://doi.org/10.1007/s13580-021-00403-5
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DOI: https://doi.org/10.1007/s13580-021-00403-5