Skip to main content
SpringerLink
Log in

Optimal conditions for spore germination and gametophyte and sporophyte production in the autumn fern Dryopteris erythrosora

  • Research Report
  • Published:
Horticulture, Environment, and Biotechnology Aims and scope Submit manuscript

Abstract

Dryopteris erythrosora (D. C. Eaton) Kuntze is used as an ornamental horticultural crop and landscaping plant, and it is a plant with high development value as an herbal medicine, but there are few studies to proliferate a large amount of plants. This study aimed to determine a suitable method for spore germination, gametophyte proliferation, sporophyte formation, and seedling production in D. erythrosora. Spore germination rate was highest in 1/2 × Murashige and Skoog (MS) medium (92.8%). A suitable medium for prothallus proliferation was selected based on gametophytes produced from germinated spores. A 1 × MS medium was most effective for gametophyte proliferation and active organ formation; moreover, 1 × MS medium containing 1% sucrose without activated charcoal was the most effective growth condition for inducing gametophyte growth and development. The suitable culture soil composition for sporophyte formation was investigated by varying the ratio of horticultural substrate, peat moss, perlite, and decomposed granite. We found that a 2:1 (v:v) mixture of horticultural substrate and perlite accelerated sporophyte formation (155 units). Furthermore, suitable seedlings and transplants for sporophyte seedling growth were determined by varying the ratio of horticultural substrate and decomposed granite, as well as plug tray cell size. For sporophyte seedlings, the greatest growth in root length (92.9 mm) and the most aerial and underground fresh weight (234.1 and 40.3 g, respectively) were observed in a mixture of horticultural substrate and decomposed granite at a 1:2 (v:v) ratio. Our results using tissue culture technology will be provided as a mass production method for substantial industrialization of D. erythrosora plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Baskaran X, Jeyachandran R (2012) In vitro spore germination and gametophyte growth assessment of a critically endangered fern: Pteris tripartita Sw. Pteridol Res 1:4–9

    Google Scholar 

  • Bertrand AM, Albuerne MA, Fernandez H, Gonzalez A, Sanchez-Tames R (1999) In vitro organogenesis of Polypodium cambricum. Plant Cell Tissue Organ Cult 57:65–69

    Article  Google Scholar 

  • Byun HJ, Kim YS, Kang HM, Kim IS (2012) Physico-chemical characteristics of used plug media and its effect on growth response of tomato and cucumber seedlings. J Bio Environ Control 21:207–212

    Google Scholar 

  • Camloh M (1993) Spore germination and early gametophyte development of Platycerium bifurcatum. Am Fern J 83:79–85

    Article  Google Scholar 

  • Camloh M, Gogala N (1992) In vitro culture of Platucerium bifurcatum gametophytes. Sci Hortic 51:343–346

    Article  Google Scholar 

  • Chang HC, Agrawal DC, Kuo CL, Wen JL, Chen CC, Tsay HS (2007) In vitro culture of Drynaria fortunei, a fern species source of Chinese medicine “Gu-Sui-Bu”. Vitro Cell Dev Biol Plant 43:133–139

    Article  Google Scholar 

  • Cho JS, Han JH, Lee CH (2017) Effects of medium components and composition on mass propagation of Arachniodes aristata (G. Forst.) Tindale. Hortic Sci Technol 35:131–141

    CAS  Google Scholar 

  • Cox J, Bhatia P, Ashwath N (2003) In vitro spore germination of the fern Schizaea dichotoma. Sci Hortic 97:369–378

    Article  Google Scholar 

  • De Boodt M, Verdonck O (1972) The physical properties of the substrates in horticulture. Acta Hortic 26:37–44

    Article  Google Scholar 

  • Dyer AF (1979) The culture of fern gametophytes for experimental investigation. In: Dyer AF (ed) The experimental biology of ferns. Academic, London, pp 254–305

    Google Scholar 

  • Fernandez H, Revilla MA (2003) In vitro culture of ornamental ferns. Plant Cell Tissue Organ Cult 73:1–13

    Article  CAS  Google Scholar 

  • Fernandez H, Bertrand AM, Sanchez-Tames R (1993) In vitro regeneration of Asplenium nidus L. from gametophytic and sporophytic tissue. Sci Hortic 56:71–77

    Article  Google Scholar 

  • Fernandez H, Bertrand AM, Sanchez-Tames R (1996) Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis. Plant Cell Tissue Organ Cult 45:93–97

    Article  Google Scholar 

  • Fernandez H, Bertrand AM, Sanchez-Tames R (1997) Gemmation in cultured gametophytes of Osmunda regalis. Plant Cell Rep 16:358–362

    Article  CAS  PubMed  Google Scholar 

  • Fernandez H, Bertrand AM, Sanchez-Tames R (1999) Biological and nutritional aspects involved in fern multiplication. Plant Cell Tissue Organ Cult 56:211–214

    Article  Google Scholar 

  • Jang BK, Cho JS, Lee KC, Lee CH (2017) Culture conditions affecting spore germination, prothallus propagation and sporophyte formation of Dryopteris nipponensis Koidz. Hortic Sci Technol 35:480–489

    CAS  Google Scholar 

  • Jun HJ, Kim JS, Bang JH, Kim HK, Beuchat LR, Ryu JH (2013) Combined effects of plant extracts in inhibiting the growth of Bacillus cereus in reconstituted infant rice cereal. Int J Food Microbiol 160:260–266

    Article  CAS  PubMed  Google Scholar 

  • Kim HS, Kim KH (2011) Physical properties of the horticultural substrate according to mixing ratio of peatmoss, perlite and vermiculite. Korean J Soil Sci Fert 44:321–330

    Article  Google Scholar 

  • Kim NR, Chi LW, Lee CH (2013) α-glucosidase inhibition activity of methanol extracts and fractions obtained from three Dryopteridaceae species. Korean J Med Crop Sci 21:301–305

    Article  Google Scholar 

  • Knop W (1865) Quantitative untersuchungen uber die ernahrungsprozesse der pflanzen. Landwirtsch Vers Stn 7:93–107

    Google Scholar 

  • Korea Biodiversity Information System (KBIS) (2016) Korea National Arboretum. Pocheon, Korea. http://www.nature.go.kr/index.jsp

  • Korea Fern Society (2005) Fern and fern allies of Korea. GeoBook, Seoul, pp 246–247

    Google Scholar 

  • Lee TB (2003) Coloured flora of Korea. Hyangmunsa, Seoul, p 76

    Google Scholar 

  • Martinez OG, Chambi CJ, Aviles Z (2014) Gametophytic phase of two neotropical ferns, Dennstaedtia globulifera (Poir.) Hieron and Hypolepis poeppigii Mett. ex Maxon (Dennstaedtiaceae). Plant Syst Evol 300:909–915

    Article  Google Scholar 

  • Miller JH (1968) Fern gametophytes as experimental material. Bot Rev 34:361–440

    Article  CAS  Google Scholar 

  • Moran RC (2004) A natural history of ferns. Timber Press, Portland, pp 34–89

    Google Scholar 

  • Oh SJ, Hong SS, Kim YH, Koh SC (2008) Screening of biological activities in fern plants native to jeju island. Korean J Plant Res 21:12–18

    Google Scholar 

  • Pan MJ, Staden JV (1998) The use of charcoal in in vitro culture—a review. Plant Growth Regul 26:155–163

    Article  CAS  Google Scholar 

  • Park SG, Kurosawa K, Yahata H (2009) Evaluation of water treatment residue as a soil substitute material compared to decomposed granite soil on the growth of greening trees. Bull Inst Trop Agric Kyushu Univ 32:93–100

    Google Scholar 

  • Park EY, Choi JM, Lee DH (2013) Changes in soil physical properties of peatmoss containing root media as influenced by container size and packing density. Korean J Hort Sci 31:558–564

    Google Scholar 

  • Perez-Garcia B, Mendoza-Ruiz A, Espinosa-Matias S, Gomez-Pignataro LD (2010) Gametophyte morphology of Platycerium andinum Baker and Platycerium wandae Racif. Micron 41:806–813

    Article  PubMed  Google Scholar 

  • Raghavan V (1989) Developmental biology of fern gametophytes. Cambridge University Press, Cambridge, pp 168–177

    Book  Google Scholar 

  • Ramirez-Trejo MDR, Perez-Garcia B, Mendoza-Ruiz A, Valdez-Avila R, Espinosa-Matias S (2013) Observations of the spore, gametophyte and young sporophyte of Pteridium caudatum (L.) Maxon using scanning electron microscopy. Micron 45:37–44

    Article  Google Scholar 

  • Rural Development Administration (RDA) (2002) Standard analysis of substrate. Sangnoksa, Suwon, pp 83–102

    Google Scholar 

  • Sun BY, Shin HC, Hyun JO, Kim YD, Oh SH (2014) Vascular plants of Dokdo and Ulleungdo Islands in Korea. The National Institute of Biological Resources, GeoBook, Seoul, pp 30–37

    Google Scholar 

  • Suo J, Chen S, Zhao Q, Shi L, Dai S (2015) Fern spore germination in response to environmental factors. Front Biol 10:358–376

    Article  CAS  Google Scholar 

  • Teng WL (1997) Activated charcoal affects morphgenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum. Plant Cell Rep 17:77–83

    Article  CAS  PubMed  Google Scholar 

  • Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631

    Article  CAS  Google Scholar 

  • Wu H, Liu XQ, Ji H, Chen LQ (2010) Effects of light, macronutrients, and sucrose on germination and development of the endangered fern Adiantum reniforme var. sinense (Adiantaceae). Sci Hortic 125:417–421

    Article  CAS  Google Scholar 

  • Yaseen M, Ahmad T, Sablok G, Standardi A, Hafiz IA (2013) Review: role of carbon sources for in vitro plant growth and development. Mol Biol Rep 40:2837–2849

    Article  CAS  PubMed  Google Scholar 

  • Zhang KM, Shi L, Li D, Zhang XC (2008) Development process and spore sterile culture of Pteris wallichiana Agardh. Acta Hortic Sin 35:94–98

    CAS  Google Scholar 

Download references

Acknowledgements

We are grateful to the Department of Horticultural Science, Chungbuk National University, and Brain Korea 21 Center for Bio-Resource Development for their support of and contributions to this work.

Author information

Authors and Affiliations

  1. Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, Cheongju, 28644, Korea

    Bo Kook Jang & Cheol Hee Lee

  2. Quality Control and Seed Tech Team, Quality Assurance Department, Nongwoo Bio CO., LTD, Yeoju, 12655, Korea

    Ju Sung Cho

  3. National Institute of Biological Resources, Incheon, 22689, Korea

    Hyuk Joon Kwon

Authors
  1. Bo Kook Jang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ju Sung Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hyuk Joon Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cheol Hee Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cheol Hee Lee.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 143 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jang, B.K., Cho, J.S., Kwon, H.J. et al. Optimal conditions for spore germination and gametophyte and sporophyte production in the autumn fern Dryopteris erythrosora. Hortic. Environ. Biotechnol. 60, 115–123 (2019). https://doi.org/10.1007/s13580-018-0097-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-018-0097-9

Keywords

Search

Navigation