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Comparison of artemisinin content and antioxidant activity from various organs of Artemisia species

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Abstract

One objective of this study was to compare the antioxidant activity from various organs of Artemisia species and identify new organs of Artemisia species with antioxidant activity. The various organs of the Artemisia species were analyzed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, which are widely used for evaluating antioxidant activity. A. annua, A. montana, A. stolonifera and A. gmelinii found to have strong antioxidant activity. These findings reinforce the potential of plants as valuable antioxidant sources. A second objective of this study was to compare the artemisinin content from various organs of Artemisia species and identify that a new organ of Artemisia species with high artemisinin content. Artemisinin is one of the most important phytochemicals found in the Artemisia species, and has been used for the treatment of malaria all over the world. Using high-performance liquid chromatography, we analyzed the artemisinin content from various organs of the Artemisia species. The roots of A. absinthium and A. rubripes howed high contents of artemisinin. This, roots of A. absinthium and A. rubripes are suggested as alternatives to A. annua as medicinal sources of artemisinin.

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References

  • Ali N., I. Shah, S.W. Shah, G. Ahmed, M. Shoaib, M. Junaid, W. Ali, and Z. Ahmed. 2013. Antioxidant and relaxant activity of fractions of crude methanol extract and essential oil of Artemisia macrocephala Jacquem. BMC Complem. Altern. Med. 13:1–8.

    Article  Google Scholar 

  • Bang M.H., M.W. Han, M.C. Song, J.G. Cho, H.G. Chung, T.S. Jeong, K.T. Lee, M.S. Choi, S.Y. Kim, and N.I. Baek. 2008. A cytotoxic and apoptosis-inducing sesquiterpenoids isolated from the aerial parts of Artemisia princeps Pampanini (Sajabalssuk). Chem. Pharm. Bull. 56:1168–1172.

    Article  CAS  PubMed  Google Scholar 

  • Bhakuni R.S., D.C. Jain, R.P. Sharma and S. Kumar. 2001. Secondary metabolites of Artemisia annua and their biological activity. Curr. Sci. 80:35–48.

    CAS  Google Scholar 

  • Brown G.D., G.Y. Liang, and L.K. Sy. 2003. Terpenoids from the seeds of Artemisia annua. Phytochemistry. 64:303–323.

    Article  CAS  PubMed  Google Scholar 

  • Buragohain P., N. Surineni, N.C. Barua, P.D. Bhuyan, P. Boruah, J.C. Borah, S. Laisharm, and D.S. Moirangthem. 2015. Synthesis of a novel series of fluoroarene derivatives of artemisinin as potent antifungal and anticancer agent. Bioorg. Med. Chem. Lett. 25:3338–3341.

    Article  CAS  PubMed  Google Scholar 

  • Castilho P.C., S.C. Gouveia, and A.I. Rodrigues. 2008. Quantification of artemisinin in Artemisia annua extracts by 1 H-NMR. Phytochem. Anal. 19:329–334.

    Article  CAS  PubMed  Google Scholar 

  • Chaturvedi D., A. Goswami, P.P. Saikia, N.C. Barua, and P.G. Rao. 2010. Artemisinin and its derivatives: a novel class of anti-malarial and anti-cancer agents. Chem. Soc. Rev. 39:435–454.

    Article  CAS  PubMed  Google Scholar 

  • Delabays N. 1997. Biologie de la reproduction chez l’Artemisia annua L. et génétique de la production en Artémisinine. Ph. D. Thesis, University of Lausanne.

    Google Scholar 

  • Erdemoglu N., I. Orhan, M. Kartal, N. Adigüzel, and B. Bani. 2007. Determination of artemisinin in selected Artemisia L. species of turkey by reversed phase HPLC. Rec. Nat. Prod. 1:36–43.

    CAS  Google Scholar 

  • Ferreira J.F. and J.M. Gonzalez. 2009. Analysis of underivatized artemisinin and related sesquiterpene lactones by high performance liquid chromatography with ultraviolet detection. Phytochem. Anal. 20:91–97.

    Article  CAS  PubMed  Google Scholar 

  • Ferreira J.F.S., V.D. Zheljazkov, and J.M. Gonzalez. 2013. Artemisinin concentration and antioxidant capacity of Artemisia annua distillation byproduct. Ind. Crop. Prod. 41:294–298.

    Article  CAS  Google Scholar 

  • Graham I.A., K. Besser, S. Blumer, C.A. Branigan, T. Czechowski, L. Elias, I. Guterman, D. Harvey, P.G. Isaac, A.M. Khan, T.R. Larson, Y. Li, T. Pawson, T. Penfield, A.M. Rae, D.A. Rathbone, S. Reid, J. Ross, M.F. Smallwood, V. Segura, T. Townsend, D. Vyas, T. Winzer, and D. Bowles. 2010. The genetic map of Artemisia annua L. identifies loci affecting yield of the antimalarial drug artemisinin. Science 327:328–331.

    Article  CAS  PubMed  Google Scholar 

  • Hatami T., S.A. Emami, S.S. Miraghaee, and M. Mojarrab. 2014. Total phenolic contents and antioxidant activities of different extracts and fractions from the aerial parts of Artemisia biennis willd. Iran. J. Pharm. Res. 13:551–558.

    PubMed Central  CAS  PubMed  Google Scholar 

  • Hencken C.P., A.S. Kalinda, and J.G. D’Angelo. 2009. The anti-infective and anti-cancer properties of artemisinin and its derivatives. Annu. Rep. Med. Chem. 44:359–378.

    Article  CAS  Google Scholar 

  • Hien T.T. and N.J. White. 1993. Qinghaosu. Lancet. 341:603–608.

    Article  CAS  PubMed  Google Scholar 

  • Huang D., B. Ou, and R.L. Prior. 2005. The chemistry behind antioxidant capacity assays. J. Agric. Food Chem. 53:1841–1856.

    Article  CAS  PubMed  Google Scholar 

  • Jeremic D., A. Jokic, A. Behbud, and M. Stefanovic. 1973. A new type of sesquiterpene lactone isolated from Artemisia annua L., arteannuin B. Tetrahedron Lett. 14:3039–3042.

    Article  Google Scholar 

  • Jung U.J., N.I. Baek, H.G. Chung, M.H. Bang, J.S. Yoo, T.S. Jeong, K.T. Lee, Y.J. Kang, M.K. Lee, H.J. Kim, J.Y. Yeo, and M.S. Choi. 2007. The anti-diabetic effects of ethanol extract from two variants of Artemisia princeps Pampanini in C57BL/KsJ-db/db mice. Food. Chem. Toxicol. 45:2022–2029.

    Article  CAS  PubMed  Google Scholar 

  • Kang K.M. and S.H. Lee. 2013. Effects of extraction methods on the antioxidative activity of Artemisia sp. J. Korean Soc. Food Sci. Nutr. 42:1249–1254.

    Article  CAS  Google Scholar 

  • Kang Y.J., U.J. Jung, M.K. Lee, H.J. Kim, S.M. Jeon, Y.B. Park, H.G. Chung, N.I. Baek, K.T. Lee, T.S. Jeong, and M.S. Choi. 2008. Eupatilin isolated from Artemisia princeps Pampanini, enhances hepatic glucose metabolism and pancreatic ß-cell function in type 2 diabetic mice. Diabetes Res. Clin. Pract. 82:25–32.

    Article  CAS  PubMed  Google Scholar 

  • Khlifi D., R.M. Sghaier, S. Amouri, D. Laouini, M. Hamdi, and J. Bouaiila. 2013. Composition and anti-oxidant, anti-cancer, and anti-inflammatory activities of Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L.. Food. Chem. Toxicol. 55:202–208.

    Article  CAS  PubMed  Google Scholar 

  • Kim H.J., J.Y. Seo, H.J. Suh, S.S. Lim, and J.S. Kim. 2012. Antioxidant activities of licorice-derived prenylflavonoids. Nutr. Res. Parct. 6:491–498.

    Article  Google Scholar 

  • Lai H.C., N.P. Singh, and T. Sasaki. 2013. Development of artemisinin compounds for cancer treatment. Invest. New Drugs 31:230–246.

    Article  CAS  PubMed  Google Scholar 

  • Lee I.S., D.K. Ryu, J. Lim, S. Cho, B.Y. Kang, and H.J. Choi. 2012. Artesunate activates Nrf2 pathway-driven anti-inflammatory potential through ERK signaling in microglial BV2 cells. Neurosci. Lett. 509:17–21.

    Article  CAS  PubMed  Google Scholar 

  • Lee J.H., S.H. Lee, C.G. Park, C.B. Park, O.T. Kim, A.J. Choi, Y.J. Kim, and S.W. Cha. 2013. Cultivation characteristics and variation of artemisinin contents by harvest time of Artemisia annua L. distributed in Korea. Korean J. Med. Crop. Sci. 21:433–438.

    Article  Google Scholar 

  • Lee T.B. 1980. Illustrated flora of Korea. Hyangmunsa, Seoul. p. 755–761.

    Google Scholar 

  • Lee W.T. 1996. Lineamenta florae Koreae. Academy Publishing Co., Seoul. p. 1082–1098.

    Google Scholar 

  • Li T., H. Chen, N. Wei, X. Mei, S. Zhang, D.L. Liu, Y. Gao, S.F. Bai, X.G. Liu, and Y.X. Zhou. 2012. Anti-inflammatory and immunomodulatory mechanisms of artemisinin on contact hypersensitivity. Int. Immunopharmacol. 12:144–150.

    Article  CAS  PubMed  Google Scholar 

  • Malagón F., J. Vázquez, G. Delgado, and A. Ruiz. 1997. Antimalaric effect of an alcoholic extract of Artemisia ludoviciana mexicana in a rodent malaria model. Parasitologia. 39:3–7.

    Google Scholar 

  • McArthur E.D., C.L. Pope, and D.C. Freeman. 1981. Chromosomal studies of subgenus tridentatae of Artemisia: evidence for autopolyploidy. Am. J. Bot. 68:589–605.

    Article  Google Scholar 

  • Naili M.B., R.O. Alghazeer, N.A. Saleh, and A.Y. Al-Najjar. 2010. Evaluation of antibacterial and antioxidant activities of Artemisia campestris (Astraceae) and Ziziphus lotus (Rhamnacea). Arab. J. Chem. 3:79–84.

    Article  CAS  Google Scholar 

  • Newton P. and N. White. 1999. Malaria: new developments in treatment and prevention. Annu. Rev. Med. 50:179–192.

    Article  CAS  PubMed  Google Scholar 

  • Paeshuyse J., L. Coelmont, I. Vliegen, J. Van hemel, J. Vandenkerckhove, E. Peys, B. Sas, E. De Clercq, and J. Neyts. 2006. Hemin potentiates the anti-hepatitis C virus activity of the antimalarial drug artemisinin. Biochem. Biophys. Res. Commun. 348:139–144.

    Article  CAS  PubMed  Google Scholar 

  • Park B.J., and M. Onjo. 2008. Antioxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf. Korean J. Plant Res. 21:408–412.

    Google Scholar 

  • Qian G.P., Y.W. Yang, and Q.L. Ren. 2005. Determination of artemisinin in Artemisia annua L. by reversed phase HPLC. J. Liq. Chromatogr. Relat. Technol. 28:705–712.

    Article  CAS  Google Scholar 

  • Qian Y.X., J.C. Kang, B.X. Lei, L. Wang, and Y. Huang. 2014. Screening and taxonomic identification of endophytic fungi with antitumor and antioxidant activities from Artemisia lactiflora. Zhongguo Zhong Yao Za Zhi 39:438–441.

    PubMed  Google Scholar 

  • Rustaiyan A. and S. Masoudi. 2011. Chemical constituents and biological activities of Iranian Artemisia species. Phytochem. Lett. 4:440–447.

    Article  CAS  Google Scholar 

  • Ryu J.H., H.J. Lee, Y.S. Jeong, S.Y. Ryu, and Y.N. Han. 1998. Yomogin, an inhibitor of nitric oxide production in LPS-activated macrophages. Arch. Pharm. Res. 21:481–484.

    Article  CAS  PubMed  Google Scholar 

  • Ryu J.H., S.J. Lee, M.J. Kim, J.H. Shin, S.K. Kang, K.M. Cho, and N.J. Sung. 2011. Antioxidant and anticancer activities of Artemisia annua L. and determination of functional compounds. J. Korean Food Sci. Nutr. 40:509–516.

    Article  CAS  Google Scholar 

  • Singh N.P. and H. Lai. 2001. Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sci. 70:49–56.

    Article  CAS  PubMed  Google Scholar 

  • Soomro S., V.B. Konkimalla, T. Langenberg, A. Mahringer, C. Horwedel, P. Holenya, A. Brand, C. Catin, G. Fricker, M. Dewerchin, P. Carmeliet, E.M. Conway, H. Jansen, and T. Efferth. 2011. Design of novel artemisinin-like derivatives with cytotoxic and antiangiogenic properties. J. Cell. Mol. Med. 15:1122–1135.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Tian N., J. Li, S. Liu, J. Huang, X. Li, and Z. Liu. 2012. Simultaneous isolation of artemisinin and its precursors from Artemisia annua L. by preparative RP-HPLC. Biomed. Chromatogr. 26:708–713.

    CAS  PubMed  Google Scholar 

  • Van Quekelberghe S.A.A., S.A. Soomro, J.A. Cordonnier, and F. Herwig Jansen. 2008. Optimization of an LC-MS method for the determination of artesunate and dihydroartemisinin plasma levels using liquid-liquid extraction. J. Anal. Toxicol. 32:133–139.

    Article  PubMed  Google Scholar 

  • Want M.Y., M. Islamuddin, G. Chouhan, A.K. Dasgupta, A.P. Chattopadhyay, and F. Afrin. 2014. A new approach for the delivery of artemisinin: Formulation, Characterization, and ex-vivo antileishmanial studies. J. Colloid Interface Sci. 432:258–269.

    Article  CAS  PubMed  Google Scholar 

  • World Health Organization. 2012. World marlaria report 2012. WHO, Geneva. p. 218.

    Google Scholar 

  • Wu B., K. Hu, S. Li, J. Zhu, L. Gu, H. Shen, B.D. Hambly, S. Bao, and W. Di. 2012. Dihydroartiminsin inhibits the growth and metast of epithelial ovarian cancer. Oncol. Rep. 27:101–108.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, 369-873, Korea

    Jeong Hoon Lee, Sang-Hoon Lee, Young-Guk Kim & Seon Woo Cha

  2. Converging Research Division, National Marine Biodiversity Institute of Korea, Seocheon, 325-902, Korea

    Jeong Min Lee

  3. Department of Integrative Plant Science, Chung-Ang University, Anseong, 456-756, Korea

    Sanghyun Lee

  4. Deprartment of Plant Resources, Kongju National University, Yesan, 340-702, Korea

    Seong-Min Kim

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  1. Jeong Hoon Lee
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  2. Jeong Min Lee
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  3. Sang-Hoon Lee
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Correspondence to Jeong Hoon Lee.

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Lee, J.H., Lee, J.M., Lee, SH. et al. Comparison of artemisinin content and antioxidant activity from various organs of Artemisia species. Hortic. Environ. Biotechnol. 56, 697–703 (2015). https://doi.org/10.1007/s13580-015-0143-9

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  • DOI: https://doi.org/10.1007/s13580-015-0143-9

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