Skip to main content
SpringerLink
Log in

Influence of short-term irradiation during pre- and post-grafting period on the graft-take ratio and quality of tomato seedlings

  • Research Report
  • Protected Horticulture
  • Published:
Horticulture, Environment, and Biotechnology Aims and scope Submit manuscript

Abstract

The objective of this study was to evaluate influence of short-term irradiation during pre- and post-grafting period on the graft-take ratio and quality of tomato seedlings. Irradiation by six light qualities, darkness, white fluorescent lamps (WFL), red LED, far-red LED, blue LED, and natural light, were used to treat seedlings for 10 days before grafting. And Irradiation by five light qualities, darkness, WFL, red LED, far-red LED, and blue LED, were used to treat seedlings for 10 days after grafting, during healing and acclimatization periods. When short-term irradiation was applied before grafting, the graft-take ratios (27.8–66.7%) were considerably low in all light treatments as compared with natural light (96.7%). The graft-take ratio of red LED was not statically different with WFL treatment, but higher than far-red and blue LED treatments. The lowest graft-take ratio (27.8%) was observed in darkness treatment. Changing light intensity before grafting was the cause of reduced graft-take ratios in this study. There was no significant difference among natural light, WFL, and red LED treatments in growth parameters, except for leaf chlorophyll level, leaf width, and fresh weight of root, but decreased in seedlings treated with far-red LED, blue LED, and darkness. Graft-take ratios (68.5–100.0%) were enhanced when short-term irradiation was applied after grafting. The maximum (100%) graft-take ratio was recorded in red LED treatment, but was not statistically different with the WFL treatment. The lowest graft-take ratio was also observed in the darkness treatment. Plant growth responses to red LED were also similar with those to WFL after grafting. However, when short-term irradiation was applied after grafting, the lowest values of plant growth were observed in far-red LED treatment. The plant growth parameters were similar in seedlings treated with darkness and blue LED, but lower than red LED and WFL treatments. The root morphology was improved in seedlings treated with red LED after grafting by increasing total root surface, total root length, and number of toot tips. Seedling quality increased at 35 days after transplanting in the red LED treatment by increased plant growth parameters, especially compactness and root morphology, as compared with other treatments.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  • Andrews, P.K. and C.S. Marquez. 1993. Graft incompatibility. Hort. Rev. 15:183–232.

    Google Scholar 

  • Afshari, R.T., R. Fngoshtari, and S. Kalantari. 2011. Effect of light and different plant growth regulators on induction of callus growth in rapeseed (Brassica napus L) genotypes. Plant Omics J. 4:60–67.

    Google Scholar 

  • Arsenault, J.L., S. Pouleur, C. Messier, and R. Guay. 1995. WinRHIZO, a root-measuring system with a unique overlap correction method. Hortscience 30(4):906. (Abstr.)

    Google Scholar 

  • Bula, R.J., R.C. Morrow, T.W. Tibbitts, D.J. Barta, R.W. Ignatius, and T.S. Martin. 1991. Light-emitting diodes as a radiation source for plants. Hortscience 26:203–205.

    CAS  PubMed  Google Scholar 

  • Davis, A.R., P. Perkins-Veazie, Y. Sakata, S. Lopez-Galarza, J.V. Maroto, S.G. Lee, Y.C. Huh, Z.Y. Sun, A. Miguel, R.K. Stephen, R. Cohen, and J.M. Lee. 2008. Cucurbit grafting. Critical Rev. Plant Sci. 27:50–74.

    Article  Google Scholar 

  • Goins, G.D., N.C. Yorio, M.M. Sanwo, and C.S. Brown. 1997. Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with without supplemental blue lighting. J. Exp. Bot. 48:1407–1413.

    Article  CAS  PubMed  Google Scholar 

  • Hartmann, H.T., D.E. Kester, F.T. Davies, and R.L. Geneve. 1997. Plant propagation: Principles and practices. Prentice Hall, Upper Saddle River, NJ, USA. p. 770.

    Google Scholar 

  • Huang, L.H., Y.H. Hong, X.Z. Dai, and X.W. Zhang. 2009. Effect of light quality on calluses induction and differentiation of Capsicum annuum. J. Hunan Agr. Uni. 35:615–617.

    CAS  Google Scholar 

  • Jang, Y.A., E. Goto, Y. Ishigami, B. Mun, and C.H. Chun. 2011. Effect of light intensity and relative humidity on photosynthesis, growth and graft-take of grafted cucumber seedlings during healing and acclimatization. Hort. Environ. Biotechnol. 52:331–338.

    Article  CAS  Google Scholar 

  • Johkan, M., M. Oda, and G. Mori. 2008. Ascorbic acid promotes graft-take in sweet pepper plants (Capsicum annuum L). Sci. Hort. 116:343–347.

    Article  CAS  Google Scholar 

  • Johkan, M., K. Mitukuri, S. Yamasaki, G. Mori, and M. Oda. 2009. Causes of defoliation and low survival rate of grafted sweet pepper plants. Sci. Hort. 119:103–107.

    Article  CAS  Google Scholar 

  • Johkan, M., K. Shoji, F. Goto, S. Hashida, and T. Yoshihara. 2010. Blue light-emitting diode light irradiation of seedlings improves seedling quality and growth after transplanting in red leaf lettuce. Hortscience 45:1809–1814.

    Google Scholar 

  • Keiko, O.K., T. Masahide, K. Noya, F. Kazuhiro, and K. Kenji. 2007. Effect of light quality on growth and vegetable quality in leaf lettuce, spinach, and komatsuna. Environ. Control Biol. 45:189–198.

    Article  Google Scholar 

  • Kim, Y.H., C.S. Kim, J.W. Lee, and S.G. Lee. 2001. Effect of vapor pressure deficit on the evapotranspiration rate and graft-taking of grafted seedlings population under artificial lighting. J. Bio-Env. Cont. 10:232–236.

    Google Scholar 

  • Kim, H.H., G.D. Goins, and R.M. Wheeler. 2004. Stomatal conductance of lettuce grown under or exposed to different light qualities. Ann. Bot. 94:691–697.

    Article  PubMed  Google Scholar 

  • Lee, J. and M. Oda. 2003. Grafting of herbaceous vegetable and ornamental crops. Hort. Rev. 28:61–124.

    Google Scholar 

  • Lee, J.M., C. Kubota, S.J. Tsao, Z. Bie, P. Hoyos Echevarria, L. Morra, and M. Oda. 2010. Current status of vegetable grafting: Diffusion, grafting techniques, automation. Sci. Hort. 127:93–105.

    Article  Google Scholar 

  • Li, H., Z. Xu, and C. Tang. 2010. Effect of light-emitting diodes on growth and morphogenesis of upland cotton (Gossypium hirsutum L.) plantlets in vitro. Plant Cell Tissue Organ Cult. 103:155–163.

    Article  Google Scholar 

  • McNellis, T.W. and X.W. Deng. 1995. Light control of seedling morphogenetic pattern. Plant Cell 7:1749–1761.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Moon, H.K., S.K. Park, Y.W. Kim, and C.S. Kim. 2006. Growth of Tsuru-rindo (Tripterospermum japonicum) cultured in vitro under various sources of light-emitting diode (LED) irradiation. J. Plant. Biol. 49:174–179.

    Article  Google Scholar 

  • Morgan, D.C. and H. Smith. 1979. A systematic relationship between phytochrome-controlled development and species habitat, for plants grown in simulated natural irradiation. Planta 145:253–258.

    Article  CAS  PubMed  Google Scholar 

  • Miyamoto, Y., R.E. Nisbett, and T. Masuda. 2006. Culture and the physical environment. Holistic versus analytic perceptual affordances. Psychol. Sci. 17:113–119.

    Article  PubMed  Google Scholar 

  • Neff, M.M., C. Fankhauser, and J. Chory. 2000. Light: An indicator of time and place. Genes Dev. 14:257–271.

    CAS  PubMed  Google Scholar 

  • Nicola, S. 1998. Understanding root systems to improve seedling quality. HortTechnology 8:544–549.

    Google Scholar 

  • Nobuoka, T., T. Nishimoto, and K. Toi. 2005. Wind and light promote graft-take and growth of grafted tomato seedlings. J. Japan. Soc. Hort. Sci. 74:170–175.

    Article  Google Scholar 

  • Oda, M. 1999. Grafting of vegetables to improve greenhouse production. Food Fert. Technol. Centre Ext. Bul. 480:1–11.

    Google Scholar 

  • Oda, M., M. Maruyama, and G. Mori. 2005. Water transfer at graft union of tomato plants grafted onto Solanum rootstocks. J. Japan. Soc. Hort. Sci. 74:458–463.

    Article  Google Scholar 

  • Ogata, T., Y. Kabashima, S. Shiozaki, and S. Horiuchi. 2005. Regeneration of the vascular bundle at the graft interface in auto-and heterografts with juvenile nucellar seedlings of satsuma mandarin, yuzu and trifoliate orange. J. Japan. Soc. Hort. Sci. 74:214–220.

    Article  Google Scholar 

  • Rivero, R.M., J.M. Ruiz, and L. Romero. 2003. Role of grafting in horticultural plants under stress conditions. Food Agr. Environ. 1:70–74.

    Google Scholar 

  • Tennessen, D.J., E.L. Singsaas, and T.D. Sharkey. 1994. Light-emitting diodes as a light source for photosynthesis research. Photosynthesis. Res. 39:85–92.

    Article  CAS  Google Scholar 

  • Vu, N.T., C.H. Zhang, Z.H. Xu, Y.S. Kim, H.M. Kang, and I.S. Kim. 2013. Enhanced graft-take ratio and quality of grafted tomato seedlings by controlling temperature and humidity conditions. Protected Hort. Plant Fac. 22:146–153.

    Article  Google Scholar 

  • Wang, H., M. Gu, J. Cui, K. Shi, Y. Zhou, and J. Yu. 2009. Effects of light quality on CO2 assimilation, chlorophyll-fluorescence quenching, expression of Calvin cycle genes and carbohydrate accumulation in Cucumis sativus. J. Photochem. Photobiol. B: Biol. 96:30–37.

    Article  CAS  Google Scholar 

  • Yong, S.S., J.L. Mun, S.L. Eun, H.A. Joon, H.L. Jae, J.K. Ha, W.P. Hoo, G.U. Young, D.P. So, and H.C. Jang. 2012. Effect of LEDs (light emitting diodes) irradiation on growth and mineral absorption of lettuce (Lactuca sativa L. ‘Lollo Rosa’). J. Bio-Env. Cont. 21:180–185.

    Google Scholar 

  • Yu, H. and B.L. Ong. 2003. Effect of radiation quality on growth and photosynthesis of Acacia mangium seedlings. Photosynthetica 41:349–355.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Horticulture, Kangwon National University, Chuncheon, 200-701, Korea

    Ngoc-Thang Vu, Ho-Min Kang & Il-Seop Kim

  2. Department of Plant and Food Science, Sangmyung University, Cheonan, 330-720, Korea

    Young-Shik Kim

Authors
  1. Ngoc-Thang Vu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Young-Shik Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ho-Min Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Il-Seop Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Il-Seop Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vu, NT., Kim, YS., Kang, HM. et al. Influence of short-term irradiation during pre- and post-grafting period on the graft-take ratio and quality of tomato seedlings. Hortic. Environ. Biotechnol. 55, 27–35 (2014). https://doi.org/10.1007/s13580-014-0115-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-014-0115-5

Additional key words

Search

Navigation