Abstract
Two-year old seedlings derived from a Ligustrum quihoui plant (NA56612.01) showed variation in leaf sizes, and the leaf sizes of several L. quihoui seedlings were significantly larger than other seedlings, suggesting that possibility of interspecific hybridization with L. japonicum or L. foliosum which were maintained sympatrically with the L. quihoui at the repository. However, the leaves of seedlings were smaller than those of mature L. quihoui plants, thus requiring the use of molecular markers to verify any possible hybridization. Based on the sequence analysis of the nuclear internal transcribed spacers 1, 2 in ribosomal RNA gene (ITS 1, 2 region), the sequence of all seedlings showed 100% homology and perfectly matched with the L. quihoui accession, but not with L. japonicum or L. foliosum. These data suggested that the seedlings were not resulted from interspecific hybridization, but from intra-pollination among the L. quihoui progeny. Genetic purity of the L. quihoui can be maintained among seedlings obtained from the mother plant. This is an important feature for ex situ germplasm conservation of L. quihoui.
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Ma, S.H., Burchi, G., Suh, J.K. et al. Identification of Ligustrum seedlings based on sequence analysis of an internal transcribed spacer. Hortic. Environ. Biotechnol. 55, 423–427 (2014). https://doi.org/10.1007/s13580-014-0058-x
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DOI: https://doi.org/10.1007/s13580-014-0058-x