Skip to main content
SpringerLink
Log in

Genetic identification of apple cultivars bred in Korea using simple sequence repeat markers

  • Research Report
  • Published:
Horticulture, Environment, and Biotechnology Aims and scope Submit manuscript

Abstract

Among the many DNA markers currently available, simple sequence repeat (SSR) markers are well developed and widely used for gene identification and cultivar discrimination by apple breeders and researchers. We developed SSR fingerprints for identification and to reveal the genetic diversity of 22 apple cultivars released from Korea. We used a total of 22 SSR markers. Among them, 15 SSR markers were developed from the complete sequence of ‘Golden Delicious’ and 7 SSR markers were chosen from the HiDRAS database. Except for somatic mutants, cultivar identification of all Korean bred apple genotypes was accomplished by using only two SSR markers (CH03d07 and KFBG-7). Parent-offspring relationships analysis was performed to investigate the real parents of three suspicious cultivars (‘Gamhong’, ‘Hongan’, and ‘Green Ball’). Based on the SSR markers experiment and reported S-RNase allele, we confirmed that ‘Fuji’ was used as paternal parent of ‘Gamhong’ and either ‘Hongro’ or ‘Natumidori’ were used as paternal parent of ‘Hongan’. Moreover, ‘Jonathan’ rather than ‘Fuji’ seems to be a paternal parent of ‘Green Ball’.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cho, K.H., S. Heo, J.H. Kim, I.S. Shin, S.H. Kim, D.H. Kim, S.E. Han, and H.R. Kim. 2010. Analysis of genetic diversity of apple cultivars using RAPD and SSR markers. Kor. J. Breed. Sci. 42: 525–533.

    Google Scholar 

  • Ciofi, C., S.M. Funk, T. Coote, D.J. Cheesman, R.L. Hammond, I.J. Saccheri, and M.W. Bruford. 1998. Genotyping with microsatellite markers, p. 195–201. In: A. Karp, P.G. Isaac, and D.S. Ingram. (eds.). Molecular tools for screening biodiversity. Chapman and Hall, London.

  • Cooke, R.J. 1995. Varietal identification of crop plants, p. 33–63. In: J.H. Skerritt and R. Appels (eds.). New diagnostics in crop sciences. CAB Int. Wallingford, UK.

  • Doyle, J.J. and J.L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13–15.

    Google Scholar 

  • Galli, Z., G. Halász, E. Kiss, L. Heszky, and J. Dobránszki. 2005. Molecular identification of commercial apple cultivars with microsatellite markers. HortScience 40:1974–1977.

    CAS  Google Scholar 

  • Gianfranceschi, L., N. Seglias, R. Tarchini, M. Komjanc, and C. Gessler. 1998. Simple sequence repeats for the genetic analysis of apple. Theor. Appl. Genet. 96:1069–1076.

    Article  CAS  Google Scholar 

  • Guilford, P., S. Prakash, J.M. Zhu, E. Rikkerink, S. Gardiner, H. Bassett, and R. Forster. 1997. Microsatellites in apple and pear. J. Amer. Soc. Hort. Sci. 128:515–520.

    Google Scholar 

  • Heo, S., S.E. Han, S.I. Kwon, J.H. Jun, M.J. Kim, and H.J. Lee. 2011. Identification of S-allele genotypes of Korean apple cultivars by using allele-specific polymerase chain reaction. Hort. Environ. Biotechnol. 52:158–162.

    Article  Google Scholar 

  • Hokanson, S.C., A.K. Szewc-McFadden, W.F. Lamboy, and J.R. McFerson. 1998. Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus × domestica Borkh. core subset collection. Theor. Appl. Genet. 97:671–683.

    Article  CAS  Google Scholar 

  • Kiraly, I., M. Toth, A. Pedrye, J. Halasz, and T. Deak. 2009. Parent identification of Hungarian apple cultivars using SSR markers. Acta Hort. 839:471–477.

    CAS  Google Scholar 

  • Kitahara, K., S. Matsumoto, T. Yamamoto, J. Seojima, T. Kimura, H. Komatsu, and K. Abe. 2005. Parent Identification of eight apple cultivars by S-RNase analysis and simple sequence repeat Markers. HortScience 40:314–317.

    CAS  Google Scholar 

  • Laurens, F., C.E. Durel, and M. Lascostes. 2004. Molecular characterization of french local apple cultivars using SSRs. Acta Hort. 663:639–642.

    CAS  Google Scholar 

  • Liebhard, R., L. Gianfranceschi, B. Koller, C.D. Ryder, R. Tarchini, E. Van de Weg, and C. Gessler. 2002. Development and characterisation of 140 new microsatellites in apple (Malus × domestica Borkh.). Mole. Breed. 10:217–241.

    Article  CAS  Google Scholar 

  • Lodhi, M.A., G.N. Ye, N.F. Weeden, and B.I. Reisch. 1994. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species and Ampelopsis. Plant Mol. Biol. Rep. 12:6–13.

    Article  CAS  Google Scholar 

  • Silfverberg-Dilworth, E., C.L. Matasci, W.E. Van de Weg, M.P.W. Van kaauwn, M. Walser, L.P. Kodde, V. Soglio, L. Gianfranceschi, C.E. Durel, F. Costa, T. Yamamoto, B. Koller, C. Gesster, and A. Patochi. 2006. Microsatellite markers spanning the apple (Malus × domestica Borkh.) genome. Tree Genet. Genomes 2:202–224.

    Article  Google Scholar 

  • Sitther, V., D. Zhang, S.A. Dhekney, D.L. Harris, A.K. Yadav, and W.R. Okie. 2012. Cultivar identification, pedigree verification, and diversity analysis among peach cultivars based on simple sequence repeat markers. J. Amer. Soc. Hort. Sci. 137:114–121.

    Article  CAS  Google Scholar 

  • This, P., A. Jung, P. Boccacci, J. Borrego, R. Botta, L. Costantini, M. Crespan, G.S. Dangl, C. Eisenheld, F. Ferreira-Monteiro, S. Grando, J. Ibáñez, T. Lacombe, V. Laucou, M. Magalhães, C.P. Meredith, N. Milani, E. Peterlunger, F. Regner, L. Zulini, and E. Maul. 2004. Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theor. Appl. Genet. 109:1448–1458.

    Article  CAS  PubMed  Google Scholar 

  • Ukai, Y. 2004. The theory of cultivar identification in plant (In Japanese). Agricul. Hortic. 79:194–198.

    Google Scholar 

  • International Union for the Protection of New Varieties of Plants (UPOV). 2006. Guidelines for DNA-profiling: Molecular marker selection and database construction (“BMT guidelines” project 6). UPOV, Geneva, Switzerland.

    Google Scholar 

  • Velasco, R., A. Zharkikh, J. Affourtit, A. Dhingra, A. Cestaro, A. Kalyanaraman, P. Fontana, S.K. Bhatnagar, M. Troggio, D. Pruss, S. Salvi, M. Pindo, P. Baldi, S. Castelletti, M. Cavaiuolo, G. Coppola, F. Costa, V. Cova, A. Dal Ri, V. Goremykin, M. Komjanc, S. Longhi, P. Magnago, G. Malacarne, M. Malno, D. Micheletti, M. Moretto, M. Perazzolli, A. Si-Ammour, S. Vezzulli, E. Zini, G. Eldredge, L.M. Fitzgerald, N. Gutin, J. Lanchbury, T. Macalma, J.T. Mitchell, J. Reid, B. Wardell, C. Kodira, Z. Chen, B. Desany, F. Niazi, M. Palmer, T. Koepke, D. Jiwan, S. Schaeffer, V. Krishnan, C. Wu, V.T. Chu, S.T. King, J. Vick, Q. Tao, A. Mraz, A. Stormo, K. Stormo, R. Bogden, D. Ederle, A. Stella, A. Vecchietti, M.M. Kater, S. Masiero, P. Lasserre, Y. Lespinasse, A.C. Allan, V. Bus, D. Chagné, R.N. Crowhurst, A.P. Gleave, E. Lavezzo, J.A. Fawcett, S. Proost, P. Rouzé, L. Sterck, S. Toppo, B. Lazzari, R.P. Hellens, C.E. Durel, A. Gutin, R.E. Bumgarner, S.E. Gardiner, M. Skolnick, M. Egholm, Y. Van de Peer, F. Salamini, and R. Viola. 2010. The genome of the domesticated apple (Malus × domestica Borkh.). Nat. Genet. 42:833–839.

    Article  CAS  PubMed  Google Scholar 

  • Wichmann, B., Z. Galli, S. Molnár, Z. Galbács, E. Kiss, T. Szabó, and L. Heszky. 2007. Molecular identification of old hungarian apple varieties. Int. J. Hort. Sci. 13:37–42.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Agriculture and Life Science, Kyungpook National University, Daegu, 702-701, Korea

    Seung Hyun Ban, Won Ho Yun, Gi Hoon Kim & Cheol Choi

  2. Apple Experiment Station, National Institute of Horticultural & Herbal Science, Rural Development Administration, Gunwi, 716-812, Korea

    Soon Il Kwon

Authors
  1. Seung Hyun Ban
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Won Ho Yun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gi Hoon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Soon Il Kwon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cheol Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cheol Choi.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ban, S.H., Yun, W.H., Kim, G.H. et al. Genetic identification of apple cultivars bred in Korea using simple sequence repeat markers. Hortic. Environ. Biotechnol. 55, 531–539 (2014). https://doi.org/10.1007/s13580-014-0017-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-014-0017-6

Additional key words

Search

Navigation