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Evaluation of genetic diversity of parental lines for development of heterotic groups in hybrid rice (Oryza sativa L.)

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Abstract

Knowledge of genetic diversity and potential heterotic relationships among parental lines is of significant importance in hybrid rice breeding programs. In the present study, in order to understand the genetic diversity among 96 parental lines, they were characterized for their diversity with respect to their morphological traits (n = 12) and molecular markers using a set of 50 SSR markers. Morphological diversity was estimated using Mahalanobis D2 statistics in terms of generalized group distance. Based on morphological diversity analysis, the 96 lines were grouped into 5 major and 13 monogenotypic clusters. In molecular marker analysis, the parental lines were consistently clustered into B (Maintainer group) and R (Restorer group) groups based on distance and model based approaches. Strong correspondence was observed between the pedigree of parental lines with molecular genotyping based grouping than morphological trait based grouping. From the results of the present investigation, it is evident that the available diversity among the two groups i.e., maintainer group (B) and restorer group (R) is sufficient for developing heterotic hybrids, but within the maintainer and restorer groups, the diversity is limited, the diversity among restorers was moderate, while it was low among the maintainers and hence efforts are needed for broadening their genetic base of parental lines for development and adoption of high-yielding hybrids.

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Abbreviations

SSR:

Simple sequence repeats

GCP:

Generation challenge programme

CTAB:

Cetyl trimethyl ammonium bromide

PIC:

Polymorphic information content

PCA:

Principal component analysis

AMOVA:

Analysis of molecular variance

UPGMA:

Unweighted pair group method with arithmetic mean

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Acknowledgements

We are grateful to Director, ICAR-IIRR, Hyderabad for guidance, providing research facilities and funding to conduct the experiment.

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Authors and Affiliations

  1. Crop Improvement Section, ICAR-Indian Institute of Rice Research, Rajendranagar, Hyderabad, 500030, India

    K. Sruthi, P. Senguttuvel, P. Revathi, K. B. Kemparaju, P. Koteswararao & A. S. Hari Prasad

  2. Genetics Division, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Vikram Jeet Singh, E. Ranjith Kumar, Prolay Kumar Bhowmick, K. K. Vinod, S. Gopala Krishnan & A. K. Singh

  3. ICAR- National Professor Project, ICAR- Indian Institute of Rice Research, Hyderabad, India

    B. Divya

  4. Biotechnology, Crop Improvement Section, ICAR-IIRR, Rajendranagar, Hyderabad, 500030, India

    R. M. Sundaram

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  1. K. Sruthi
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Contributions

AKS and ASH conceptualised the experiment; KS conducted the lab and field experiment; KS and PK collected and compiled data; KS, BD, RKE, VJS, PKB, KKV and GKS molecular and field data analysis; KS, BD, ASH, RMS, PS, PR and KBK, RKE wrote and corrected the manuscript.

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Correspondence to A. S. Hari Prasad.

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Sruthi, K., Divya, B., Senguttuvel, P. et al. Evaluation of genetic diversity of parental lines for development of heterotic groups in hybrid rice (Oryza sativa L.). J. Plant Biochem. Biotechnol. 29, 236–252 (2020). https://doi.org/10.1007/s13562-019-00529-9

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