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Lipid desaturation in prokaryotic pathway abates the high-oleic phenotype of FAD2-silenced tobacco at lower temperature

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Abstract

The stability of the high-oleic characteristic of tobacco (Nicotiana tabacum) transformants obtained by silencing of microsomal Ω-6 desaturase (FAD2) was investigated when grown at low temperature. Results showed that oleic acid content in total leaf lipids, individual leaf lipids and total seed lipids of transformants decreased drastically at low temperature, with a corresponding increase of polyunsaturated fatty acid contents. The decrease of oleate was caused by neither the restoration of FAD2 transcripts nor the disappearance of small interference RNA. A significant increase of hexadecadienoic acid and hexadecatrienoic acid was observed in MGDG (monogalactosyldiacylglycerol) of transformants grown at lower temperature. Hexadecadienoic and hexadecatrienoic acids, typical fatty acids in MGDG, are produced through the activity of Ω-6 desaturase in plastids (FAD6). The significant increase of Hexadecadienoic and hexadecatrienoic acids suggested the involvement of FAD6 and/or other desaturases in the lipid desaturation of transgenic plants under lower temperature, leading to a significant decrease of oleate in plant cells. These results suggested that the activities of plastidic desaturases must be, at least partially, responsible for the instability of the high-oleic characteristic of FAD2-silenced plants.

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Abbreviations

DGDG:

Digalactosyldiacylglycerol

MGDG:

Monogalactosyldiacylglycerol

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

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Acknowledgments

This work was supported by the National Sciences Foundation of China under Grant numbers 31370674, 31300620, and 31200462; Foundation of Beijing Municipal Education Committee under Grant numbers KM201410020001 and KM20130020002; Funding Project for Scientific Research Quality Improvement in Beijing University of Agriculture (GJB2013001); and Training Programme Foundation for the Beijing Municipal Excellent Talents under Grant number 2013D005021000003.

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Authors and Affiliations

  1. Key Laboratory of Urban Agriculture (North) Ministry of Agriculture, Beijing University of Agriculture, Beijing, 102206, China

    Lulu Zhang, Heshu Lu, Chunmei Liu, Feiyan Xue, Lanqing Ma & Mingfeng Yang

  2. School of Chemical and Environmental Engineering, North University of China, Taiyuan, Shanxi, 030051, China

    Jing Yang

Authors
  1. Lulu Zhang
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  2. Heshu Lu
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  3. Chunmei Liu
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  4. Feiyan Xue
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  5. Jing Yang
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  6. Lanqing Ma
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  7. Mingfeng Yang
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Corresponding author

Correspondence to Mingfeng Yang.

Additional information

Lulu Zhang and Heshu Lu contributed equally to this work.

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Zhang, L., Lu, H., Liu, C. et al. Lipid desaturation in prokaryotic pathway abates the high-oleic phenotype of FAD2-silenced tobacco at lower temperature. J. Plant Biochem. Biotechnol. 25, 375–381 (2016). https://doi.org/10.1007/s13562-016-0349-7

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  • DOI: https://doi.org/10.1007/s13562-016-0349-7

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