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Molecular characterization and assessment of genetic diversity of inbred lines showing variability for drought tolerance in maize

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Abstract

A set of 24 genotypes bred at different centres in India as well as in CIMMYT showing variability for drought tolerance were selected for molecular and morpho-physiological characterization. A set of 35 SSR markers, having genome-wide coverage, was chosen for genotyping the inbreds. These markers generated a total of 111 polymorphic alleles with an average of 3.17 alleles per locus. The minimum and maximum PIC value was 0.27 and 0.77 with a mean of 0.5. A total of 13 unique alleles were found in the 24 inbred lines. The coefficient of genetic dissimilarity ranged from 0.192 to 0.803. NJ-based tree suggested the presence of three major clusters of which, two of them had subgroups. Phenotyping of inbreds by morpho-physiological traits revealed that there was a positive relationship among root length, chlorophyll content, relative water content while anthesis-silking interval was negative relationship with all these traits. Genotyping data complemented by morpho-physiological parameters were used to identify a number of pair-wise combinations for the development of mapping population segregating for drought tolerance and potential heterotic pairs for the development of drought tolerant hybrids.

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Abbreviations

RWC:

Relative water content

ASI:

Anthesis-silking interval

RL:

Root length

CC:

Chlorophyll content

LAI:

Leaf area index

QTL:

Quantitative trait locus

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Acknowledgement

The authors sincerely acknowledge the National Agricultural Innovation Project (NAIP) for their financial support to carry out this research.

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Authors and Affiliations

  1. Maize Genetics Unit, Division of Genetics, Indian Agricultural Research Institute, New Delhi, 110012, India

    T. Nepolean, Firoz Hossain, Neha Pandey & H. S. Gupta

  2. Directorate of Maize Research, PUSA, New Delhi, 110012, India

    Ishwar Singh

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  1. T. Nepolean
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Correspondence to T. Nepolean.

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Nepolean, T., Singh, I., Hossain, F. et al. Molecular characterization and assessment of genetic diversity of inbred lines showing variability for drought tolerance in maize. J. Plant Biochem. Biotechnol. 22, 71–79 (2013). https://doi.org/10.1007/s13562-012-0112-7

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