Skip to main content
SpringerLink
Log in

In vitro effects of Geranium graveolens, Sarcopoterium spinosum and Varthemia iphionoides extracts on pancreatic MIN6 proliferation and insulin secretion and on extrapancreatic glucose diffusion

  • Original Article
  • Published:
International Journal of Diabetes in Developing Countries Aims and scope Submit manuscript

Abstract

Geranium graveolens, Sarcopoterium spinosum and Varthemia iphionoides are utilized traditionally for diabetes ethnomedicine in Jordan. Their aqueous extracts (AE) for antidiabetic activity and mechanism of action were investigated in vitro. Insulin secretion and pancreatic proliferation as well as glucose diffusion in vitro bioassays were recruited. Similar to L-alanine, S. spinosum AE (0.01 and 0.5 mg/ml) potentiated acute glucose-stimulated Ca2+ regulated insulin secretion in the clonal pancreatic β-cell line MIN6 (19.3 and 20.6 folds, P < 0.001). G. graveolens and V. iphionoides AEs were inactive. Comparable to GLP-1-enhanced β-cell proliferation, G. graveolens AE (0.01 and 0.05 mg/ml), S. spinosum (0.1 mg/ml) and V. iphionoides AEs (0.5 and 1 mg/ml) induced augmentations in pancreatic BrdU incorporation (P < 0.05−0.001). Performing effectively as guar gum diffusional hindrance, all three plants’ AE concentrations retarded 24 h glucose efflux into external solution across dialysis membrane (P < 0.05−0.001). Conclusively β-cell mass expansion was augmented and carbohydrate absorption was reduced by all selected plants. Exceptionally, S. spinosum stimulated pancreatic insulin secretion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. UK Prospective Diabetes Study (UKPDS) Group. Intensive blood-glucose control with sulphonylureas or insulin compared with conventional treatment and risk of complications in patients with type 2 diabetes (UKPDS 33). Lancet. 1998;352:837–53.

    Google Scholar 

  2. Levy P. The current unmet need in type 2 diabetes mellitus: addressing glycaemia and cardiovascular disease. Postgrad Med. 2009;121:7–12.

    Article  PubMed  Google Scholar 

  3. Ball AJ, McCluskey JT, Flatt PR, McClenaghan N. Chronic exposure to tolbutamide and glibenclamide impairs insulin secretion but not transcription of KATP channel components. Pharmacol Res. 2004;50:41–6.

    Article  PubMed  CAS  Google Scholar 

  4. Al-Aboudi A, Afifi FU. Plants used for the treatment of diabetes in Jordan: A review of scientific evidence. Pharma Biol. 2011;49:221–39.

    Article  Google Scholar 

  5. Wazaify M, Afifi FU, El-Khateeb M, Ajlouni K. Complementary and alternative medicine use among Jordanian diabetes patients. Complement Ther Clin Pract. 2011;17:71–5.

    Article  PubMed  Google Scholar 

  6. Ajlouni K, Khader YS, Batieha A, Ajlouni H, El-Khateeb M. An increase of diabetes mellitus in Jordan over 10 years. J Diabetes Complications. 2008;22:317–24.

    Article  PubMed  Google Scholar 

  7. Zindah M, Belbeisi A, Walke H, Mokdad AH. Obesity and diabetes in Jordan: findings from the behavioral risk factor surveillance system, 2004. Prev Chron Disease. 2008;5:1–8.

    Google Scholar 

  8. Oran SA, Al-Eisawi DM. Check list of medicinal plants in Jordan. Dirasat. 1998;25:84–112.

    Google Scholar 

  9. Hamdan II, Afifi FU. Screening of Jordanian flora for α-amylase inhibitory activity. Pharm Biol. 2008;46:746–50.

    Article  CAS  Google Scholar 

  10. Flammang AM, Kendall DM, Baumgartner CJ, Slagle TD, Choe YS. Effect of a viscous fiber bar on postprandial glycemia in subjects with type 2 diabetes. J Am Coll Nutr. 2006;25:409–14.

    Article  PubMed  CAS  Google Scholar 

  11. Khookhor O, Sato Y. Mongolian plant extracts with potential glucose absorption inhibiting effects in rats. J Trad Med. 2009;26:74–9.

    Google Scholar 

  12. Gupta P, Premavalli KS. In-vitro studies on functional properties of selected natural dietary fibers. Int J Food Propert. 2011;14:397–410.

    Article  CAS  Google Scholar 

  13. Gallagher AM, Flatt PR, Duggy G, Abdel-Wahab YHA. The effects of traditional antidiabetic plants on in vitro glucose diffusion. Nutr Res. 2003;23:413–24.

    Article  CAS  Google Scholar 

  14. Gorelick J, Kitron A, Pen S, Rosenzweig T, Madar Z. Anti-diabetic activity of Chiliadenus iphionoides. J Ethnopharmacol. 2011;137:1245–9.

    Article  PubMed  Google Scholar 

  15. Smirin P, Taler D, Abitbol G, Brutman-Barazani T, Kerem Z, Sampson SR, et al. Sarcopoterium spinosum extract as an antidiabetic agent: in vitro and in vivo study. J Ethnopharmacol. 2010;129:10–7.

    Article  PubMed  Google Scholar 

  16. Kasabri V, Flat PR, Abdel-Wahab YH. Terminalia bellirica stimulates the secretion and action of insulin and inhibits starch digestion and protein glycation in vitro. Br J Nutr. 2010;103:212–7.

    Article  PubMed  CAS  Google Scholar 

  17. Mathews JN, Flatt PR, Abdel-Wahab YH. Asparagus adscendes (Shwera musali) stimulates insulin secretion, insulin action and inhibits starch digestion. Br J Nutr. 2006;95:576–81.

    Article  PubMed  CAS  Google Scholar 

  18. Hamdan II, Afifi FU. Studies on the in vitro and in vivo hypoglycaemic activities of some medicinal plants used in treatment of diabetes in Jordanian traditional medicine. J Ethnopharmacol. 2004;93:117–21.

    Article  PubMed  CAS  Google Scholar 

  19. Miyazaki J, Araki K, Yamato E, Ikegami H, Asano T, Shibasaki Y, et al. Establishment of a pancreatic β cell line that retains glucose inducible insulin secretion: Special reference to expression of glucose transport forms. Endocrinology. 1990;127:126–32.

    Article  PubMed  CAS  Google Scholar 

  20. Hannan JM, Ali L, Rokeya B, Khaleque J, Akhter M, Flatt PR, et al. Soluble dietary fibre fraction of Trigonella foenum-graecum (fenugreek) seed improves glucose homeostasis in animal models of type 1 and type 2 diabetes by delaying carbohydrate digestion and absorption, and enhancing insulin action. Br J Nutr. 2007;97:514–21.

    Article  PubMed  CAS  Google Scholar 

  21. List JF, Habener JF. Glucagon-like peptide 1 agonists and the development and growth of pancreatic β-cells. Am J Physiol Endocrinol Metab. 2004;286:E875–81.

    Article  PubMed  CAS  Google Scholar 

  22. Thiebart-Fassy I, Hervagault J. Combined effects of diffusional hindrances, electronic repulsion and product inhibition on the kinetic properties of a bound acid phosphatase. FEBS. 1993;334:89–94.

    Article  CAS  Google Scholar 

  23. Takahashi T, Yokawa T, Ishihara N, Okubo T, Chu DC, Nishigaki E, et al. Hydrolysed guar gum decreases postprandial blood glucose and glucose absorption in the rat small intestine. Nutr Res. 2009;29:419–25.

    Article  PubMed  CAS  Google Scholar 

  24. Wagner H, Bladt S. Plant drug analysis, a thin layer chromatography atlas. Berlin, Springer-Verlag, 1996; 155, 176, 178.

  25. Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1995;2:137–89.

    Article  PubMed  CAS  Google Scholar 

  26. Rosata A, Vitali C, De Laurentis N, Armenise D, Antonietta MM. Antibacterial effect of some essential oils administered alone or in combination with Norfloxacin. Phytomedicine. 2007;14:727–32.

    Article  Google Scholar 

  27. Kasabri V, Afifi FU, Hamdan I. Evaluation of the acute antihyperglycemic effects of four selected indigenous plants from Jordan used in traditional medicine. Pharm Biol. 2011;49:687–95.

    Article  PubMed  Google Scholar 

  28. Al-Mustafa AH, Al-Thunibat OY. Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes. Pak J Biol Sci. 2008;11:351–8.

    Article  PubMed  Google Scholar 

  29. Kasabri V, Afifi FU, Hamdan I. In vitro and in vivo acute antihyperglycemic effects of five selected indigenous plants from Jordan used in traditional medicine. J Ethnopharmacol. 2011;13:888–96.

    Article  Google Scholar 

  30. Cefalu WT. Pharmacotherapy for the treatment of patients with type 2 diabetes mellitus: rationale and specific agents. Nature. 2007;81:636–49.

    CAS  Google Scholar 

  31. Al-Dabbas MM, Suganuma T, Kitahara K, Hou DX, Fujii M. Cytotoxic, antioxidant and antibacterial activities of Varthemia iphionoides Boiss. Extracts J Ethnopharmacol. 2006;108:287–93.

    Article  Google Scholar 

  32. Afifi F, Aburjai T. Antiplatelet activity of Varthenmia iphionoides. Fitoterapia. 2004;75:629–33.

    Article  PubMed  CAS  Google Scholar 

  33. Abu-Hijleh A, Jarrar N, Adwan K. Antibacterial activity of common Varthemia, Varthemia iphionoides ethanol extract alone and in combination with cefotaxine. Adv Biol Res. 2009;3:144–7.

    CAS  Google Scholar 

  34. Al-Dabbas MM, Kitahara K, Suganuma T, Hashimoto F, Tadera K. Antioxidant and alpha-amylase inhibitory compounds from aerial parts of Varthemia iphionoides. Boiss Biosci Biotechnol Biochem. 2006;70:2178–84.

    Google Scholar 

  35. Sjoholm S. Liraglutide therapy for type 2 diabetes: overcoming unmet needs. Pharmaceuticals. 2010;3:764–81.

    Article  Google Scholar 

  36. Movassat J, Portha B. Models for pharmacological activation of beta-cell regeneration in diabetes. Drug Discov Today: Disease Models. 2007;4:31–8.

    Article  Google Scholar 

  37. Dor Y, Brown J, Martinez OI, Melton DA. Adult pancreatic β-cells are formed by self-duplication rather than stem cell differentiation. Nature. 2004;429:41–6.

    Article  PubMed  CAS  Google Scholar 

  38. Fu Z, Zhang W, Zhen W, Lum H, Nadler J, Bassaganya-Riera J, et al. Genistein induces pancreatic β-cell proliferation through activation of multiple signaling pathways and prevents insulin-deficient diabetes in mice. Endocrinology. 2010;151:3026–37.

    Article  PubMed  CAS  Google Scholar 

  39. Krisanapun C, Peungvicha P, Temsiririrkkul R, Wongkrajang Y. Aqueous extract of Abutilon indicum sweet inhibits glucose absorption and stimulates insulin secretion in rodents. Nutr Res. 2009;29:579–89.

    Article  PubMed  CAS  Google Scholar 

  40. Giacco R, Clemente G, Riccardi G. Dietary fiber in treatment of diabetes: myth or reality? Dig Liver Dis. 2002;34 Suppl 2:S140–4.

    Article  PubMed  Google Scholar 

  41. Kim M. High-methoxyl pectin has greater enhancing effect on glucose uptake in intestinal perfused rats. Nutrition. 2005;21:372–7.

    Article  PubMed  CAS  Google Scholar 

  42. Butt MS, Ahmad A, Sharif MK. Influence of pectin and guar gum composite flour on plasma biochemical profile of streptozocin-induced diabetic male albino rats. Int J Food Propert. 2007;10:345–61.

    Article  CAS  Google Scholar 

  43. Flourie B, Vidon N, Florent CH, Bernier JJ. Effect of pectin on jejunal glucose absorption and unstirred layer thickness in normal man. Gut. 1984;2:936–41.

    Article  Google Scholar 

  44. Jenkins DJA, Leeds AR, Wolever TMS, Goff DV, George K, Alberti MM, et al. Unabsorbable carbohydrates and diabetes: decreased postprandial hyperglycaemia. Lancet. 1976;2:172–4.

    Article  PubMed  CAS  Google Scholar 

  45. Edwards CA, Blackburn NA, Craigne L, Davison P, Tomlin J, Sugden K, et al. Viscosity of food gums determined in vitro related to their hypoglycaemic actions. Am J Clin Nutr. 1987;46:72–7.

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Deanship of Academic Research, The University of Jordan [Grant numbers 218/2007–2008 and 29/2010–2011]. The authors thank Professor Jun-ichi Miyazaki, Osaka University, Japan for the gift of MIN6 cells and information on culturing. Further gratitude goes to Professor Peter Jones, King’s College London, UK. The technical assistance of Ismail Abaza and Dana AlQudah is acknowledged.

Conflict of interest

None.

Contributors’ detail

Violet Kasabri, Rana Abu-Dahab and Fatma U. Afifi conceptualized and designed the study, performed statistical analyses, edited and reviewed manuscript, performed the literature survey and phytochemical analyses.

Randa Naffa, Lara Majdalawi and Hazar Shawash performed the experiments.

Author information

Authors and Affiliations

  1. Faculty of Pharmacy, The University of Jordan, Queen Rania Al-Abdullah Street, Amman, 11942, Jordan

    Violet Kasabri, Rana Abu-Dahab, Fatma U. Afifi, Lara Majdalawi & Hazar Shawash

  2. Faculty of Medicine, The University of Jordan, Queen Rania Al-Abdullah Street, Amman, 11942, Jordan

    Randa Naffa

Authors
  1. Violet Kasabri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rana Abu-Dahab
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fatma U. Afifi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Randa Naffa
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Lara Majdalawi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hazar Shawash
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fatma U. Afifi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kasabri, V., Abu-Dahab, R., Afifi, F.U. et al. In vitro effects of Geranium graveolens, Sarcopoterium spinosum and Varthemia iphionoides extracts on pancreatic MIN6 proliferation and insulin secretion and on extrapancreatic glucose diffusion. Int J Diabetes Dev Ctries 33, 170–177 (2013). https://doi.org/10.1007/s13410-013-0131-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13410-013-0131-5

Keywords

Search

Navigation