Abstract
Purpose
The t(4;11)(q21;q23) translocation characterizes a form of acute lymphoblastic leukemia with a poor prognosis. It results in a fusion gene encoding a chimeric transcription factor, MLL-AF4, that deregulates gene expression through a variety of still controversial mechanisms. To provide new insights into these mechanisms, we examined the interaction between AF4, the most common MLL fusion partner, and the scaffold protein 14-3-3θ, in the context of t(4;11)-positive leukemia.
Methods
Protein-protein interactions were analyzed using immunoprecipitation and in vitro binding assays, and by fluorescence microscopy in t(4;11)-positive RS4;11 and MV4–11 leukemia cells and in HEK293 cells. Protein and mRNA expression levels were determined by Western blotting and RT-qPCR, respectively. A 5-bromo-2′-deoxyuridine assay and an annexin V/propidium iodide assay were used to assess proliferation and apoptosis rates, respectively, in t(4;11)-positive and control cells. Chromatin immunoprecipitation was performed to assess binding of 14-3-3θ and AF4 to a specific promoter element.
Results
We found that AF4 and 14-3-3θ are nuclear interactors, that 14-3-3θ binds Ser588 of AF4 and that 14-3-3θ forms a complex with MLL-AF4. In addition, we found that in t(4;11)-positive cells, 14-3-3θ knockdown decreased the expression of MLL-AF4 target genes, induced apoptosis and hampered cell proliferation. Moreover, we found that 14-3-3θ knockdown impaired the recruitment of AF4, but not of MLL-AF4, to target chromatin. Overall, our data indicate that the activity of the chimeric transcription factor MLL-AF4 depends on the cellular availability of 14-3-3θ, which triggers the transactivating function and subsequent degradation of AF4.
Conclusions
From our data we conclude that the scaffold protein 14-3-3θ enhances the aberrant activity of the chimeric transcription factor MLL-AF4 and, therefore, represents a new player in the molecular pathogenesis of t(4;11)-positive leukemia and a new promising therapeutic target.
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References
R.K. Slany, When epigenetics kills: MLL fusion proteins in leukemia. Hematol Oncol 23, 1–9 (2005). https://doi.org/10.1002/hon.739
A. Daser, T.H. Rabbitts, The versatile mixed lineage leukaemia gene MLL and its many associations in leukaemogenesis. Semin Cancer Biol 15, 175–188 (2005). https://doi.org/10.1016/j.semcancer.2005.01.007
J.L. Huret, P. Dessen, A. Bernheim, An atlas of chromosomes in hematological malignancies. Example: 11q23 and MLL partners. Leukemia 15, 987–989 (2001)
R.L. Wright, A.T.M. Vaughan, A systematic description of MLL fusion gene formation. Crit Rev Oncol/Hematol 91, 283–291 (2014). https://doi.org/10.1016/j.critrevonc.2014.03.004
R. Marschalek, Mechanisms of leukemogenesis by MLL fusion proteins. Br J Haematol 152, 141–154 (2011). https://doi.org/10.1111/j.1365-2141.2010.08459.x
C.H. Pui, D. Campana, Age-related differences in leukemia biology and prognosis: The paradigm of MLL-AF4-positive acute lymphoblastic leukemia. Leukemia 21, 593–594 (2007). https://doi.org/10.1038/sj.leu.2404598
M.W.J.C. Jansen, L. Corral, V.H.J. van der Velden, R. Panzer-Grumayer, M. Schrappe, A. Schrauder, R. Marschalek, C. Meyer, M.L. den Boer, W.J.C. Hop, M.G. Valsecchi, G. Basso, A. Biondi, R. Pieters, J.J.M. van Dongen, Immunobiological diversity in infant acute lymphoblastic leukemia is related to the occurrence and type of MLL gene rearragement. Leukemia 21, 633–641 (2007). https://doi.org/10.1038/sj.leu.2404578
C.J. Harrison, M. Griffiths, F. Moorman, S. Schnittger, J.M. Cayuela, S. Shurtleff, E. Gottardi, G. Mitterbauer, D. Colomer, E. Delabesse and V. Casteras, A multicenter evaluation of comprehensive analysis of MLL translocations and fusion gene partners in acute leukemia using the MLL FusionChip device. (vol 173, pg 17, 2007). Cancer Genet Cytogenet 179, 167–167 (2007). https://doi.org/10.1016/j.cancergencyto.2006.09.006
C. Bueno, F.J. Calero-Nieto, X. Wang, R. Valdés-Mas, F. Gutiérrez-Agüera, H. Roca-Ho, V. Ayllon, P.J. Real, D. Arambile, L. Espinosa, R. Torres-Ruiz, A. Agraz-Doblas, I. Varela, J. de Boer, A. Bigas, B. Gottgens, R. Marschalek, P. Menendez, Enhanced hemato-endothelial specification during human embryonic differentiation through developmental cooperation between AF4-MLL and MLL-AF4 fusions. Haematologica 104, 1189–1201 (2019). https://doi.org/10.3324/haematol.2018.202044
C. Ma, L.M. Staudt, LAF-4 encodes a lymphoid nuclear protein with transactivation potential that is homologous to AF-4, the gene fused to MLL in t(4;11) leukemias. Blood 87, 734–745 (1996)
J. Gecz, A.K. Gedeon, G.R. Sutherland, J.C. Mulley, Identification of the gene FMR2, associated with FRAXE mental retardation. Nat Genet 13, 105–108 (1996)
E. Bitoun, P.L. Oliver, K.E. Davies, The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling. Hum Mol Genet 16, 92–106 (2007). https://doi.org/10.1093/hmg/ddl444
G. Esposito, A. Cevenini, A. Cuomo, F. De Falco, D. Sabbatino, F. Pane, M. Ruoppolo, F. Salvatore, Protein network study of human AF4 reveals its central role in RNA pol II-mediated transcription and in phosphorylation-dependent regulatory mechanisms. Biochem J 438, 121–131 (2011). https://doi.org/10.1042/Bj20101633
F. Erfurth, C.S. Hemenway, A.C. de Erkenez, P.H. Domer, MLL fusion partners AF4 and AF9 interact at subnuclear foci. Leukemia 18, 92–102 (2004). https://doi.org/10.1038/sj.leu.2403200
J.M. Benito, L. Godfrey, K. Kojima, L. Hogdal, M. Wunderlich, H.M. Geng, I. Marzo, K.G. Harutyunyan, L. Golfman, P. North, J. Kerry, E. Ballabio, T.N. Chonghaile, O. Gonzalo, Y.H. Qiu, I. Jeremias, L. Debose, E. O'Brien, H.L. Ma, P. Zhou, R. Jacamo, E. Park, K.R. Coombes, N.A.X. Zhang, D.A. Thomas, S. O'Brien, H.M. Kantarjian, J.D. Leverson, S.M. Kornblau, M. Andreeff, M. Muschen, P.A. Zweidler-McKay, J.C. Mulloy, A. Letai, T.A. Milne, M. Konopleva, MLL-Rearranged Acute Lymphoblastic Leukemias Activate BCL-2 through H3K79 Methylation and Are Sensitive to the BCL-2-Specific Antagonist ABT-199. Cell Reports 13, 2715–2727 (2015). https://doi.org/10.1016/j.celrep.2015.12.003
H. Okuda, B. Stanojevic, A. Kanai, T. Kawamura, S. Takahashi, H. Matsui, A. Takaori-Kondo, A. Yokoyama, Cooperative gene activation by AF4 and DOT1L drives MLL-rearranged leukemia. J Clin Invest 127, 1918–1931 (2017). https://doi.org/10.1172/JCI91406
A. Yokoyama, M. Lin, A. Naresh, I. Kitabayashi, M.L. Cleary, A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell 17, 198–212 (2010). https://doi.org/10.1016/j.ccr.2009.12.040
A. Bursen, S. Moritz, A. Gaussmann, S. Moritz, T. Dingermann, R. Marschalek, Interaction of AF4 wild-type and AF4. MLL fusion protein with SIAH proteins: Indication for t(4;11) pathobiology? Oncogene 23, 6237–6249 (2004). https://doi.org/10.1038/sj.onc.1207837
P.L. Oliver, E. Bitoun, J. Clark, E.L. Jones, K.E. Davies, Mediation of Af4 protein function in the cerebellum by Siah proteins. Proc Natl Acad Sci U S A 101, 14901–14906 (2004)
J.L. Hess, MLL, Hox genes, and leukemia: the plot thickens. Blood 103, 2870–2871 (2004)
Y.T. Tan, Y. Sun, S.H. Zhu, L. Ye, C.J. Zhao, W.L. Zhao, Z. Chen, S.J. Chen, H. Liu, Deregulation of HOX genes by DNMT3A and MLL mutations converges on BMI1. Leukemia 30, 1609–1612 (2016). https://doi.org/10.1038/leu.2016.15
R.W. Stam, P. Schneider, J.A.P. Hagelstein, M.H. van der Linden, D.J.P.M. Stumpel, R.X. de Menezes, P. de Lorenzo, M.G. Valsecchi, R. Pieters, Gene expression profiling-based dissection of MLL translocated and MLL germline acute lymphoblastic leukemia in infants. Blood 115, 2835–2844 (2010). https://doi.org/10.1182/blood-2009-07-233049
T. Rozovskaia, E. Feinstein, O. Mor, R. Foa, J. Blechman, T. Nakamura, C.M. Croce, G. Cimino, E. Canaani, Upregulation of Meis1 and HoxA9 in acute lymphocytic leukemias with the t(4 : 11) abnormality. Oncogene 20, 874–878 (2001). https://doi.org/10.1038/sj.onc.1204174
J. Faber, A.V. Krivtsov, M.C. Stubbs, R. Wright, T.N. Davis, M. van den Heuvel-Eibrink, C.M. Zwaan, A.L. Kung, S.A. Armstrong, HOXA9 is required for survival in human MLL-rearranged acute leukemias. Blood 113, 2375–2385 (2009). https://doi.org/10.1182/blood-2007-09-113597
E. Kroon, J. Krosl, A.M. Buchberg, G. Sauvageau, Hoxa9 transforms primary bone marrow cells through specific collaboration with Meis1 but not PBX1. Blood 90, 1900–1900 (1998). https://doi.org/10.1093/emboj/17.13.3714
K. Orlovsky, A. Kalinkovich, T. Rozovskaia, E. Shezen, T. Itkin, H. Alder, H.G. Ozer, L. Carramusa, A. Avigdor, S. Volinia, A. Buchberg, A. Mazo, O. Kollet, C. Largman, C.M. Croce, T. Nakamura, T. Lapidot, E. Canaani, Down-regulation of homeobox genes MEIS1 and HOXA in MLL-rearranged acute leukemia impairs engraftment and reduces proliferation. Proc Natl Acad Sci U S A 108, 7956–7961 (2011). https://doi.org/10.1073/pnas.1103154108
A.V. Krivtsov, Z. Feng, M.E. Lemieux, J. Faber, S. Vempati, A.U. Sinha, X. Xia, J. Jesneck, A.P. Bracken, L.B. Silverman, J.L. Kutok, A.L. Kung, S.A. Armstrong, H3K79 methylation profiles define murine and human MLL-AF4 leukemias. Cancer Cell 14, 355–368 (2008). https://doi.org/10.1016/j.ccr.2008.10.001
P. Isnard, N. Core, P. Naquet, M. Djabali, Altered lymphoid development in mice deficient for the mAF4 proto-oncogene. Blood 96, 705–710 (2000)
M. Mancini, N. Veljkovic, V. Corradi, E. Zuffa, P. Corrado, E. Pagnotta, G. Martinelli, E. Barbieri, M.A. Santucci, 14-3-3 ligand prevents nuclear import of c-ABL protein in chronic myeloid leukemia. Traffic 10, 637–647 (2009). https://doi.org/10.1111/j.1600-0854.2009.00897.x
C. Mackintosh, Dynamic interactions between 14-3-3 proteins and phosphoproteins regulate diverse cellular processes. Biochem J 381, 329–342 (2004). https://doi.org/10.1042/BJ20031332
V. Obsilova, J. Silhan, E. Boura, J. Teisinger, T. Obsil, 14-3-3 proteins: A family of versatile molecular regulators. Physiol Res 57, S11–S21 (2008)
M.B. Yaffe, How do 14-3-3 proteins work? - gatekeeper phosphorylation and the molecular anvil hypothesis. FEBS Lett 513, 53–57 (2002). https://doi.org/10.1016/S0014-5793(01)03288-4
J. Silhan, V. Obsilova, J. Vecer, P. Herman, M. Sulc, J. Teisinger, T. Obsil, 14-3-3 protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding. J Biol Chem 279, 49113–49119 (2004). https://doi.org/10.1074/jbc.M408671200
D.L. Bolton, R.A. Barnitz, K. Sakai, M.J. Lenardo, 14-3-3 theta binding to cell cycle regulatory factors is enhanced by HIV-1 Vpr. Biol Direct 3, 17 (2008). https://doi.org/10.1186/1745-6150-3-17
A. Brunet, F. Kanai, J. Stehn, J. Xu, D. Sarbassova, J.V. Frangioni, S.N. Dalal, J.A. DeCaprio, M.E. Greenberg, M.B. Yaffe, 14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport. J Cell Biol 156, 817–828 (2002). https://doi.org/10.1083/jcb.200112059
M. Evangelopoulos, A. Parodi, J.O. Martinez, I.K. Yazdi, A. Cevenini, A.L. van de Ven, N. Quattrocchi, C. Boada, N. Taghipour, C. Corbo, B.S. Brown, S. Scaria, X. Liu, M. Ferrari and E. Tasciotti, Cell source determines the immunological impact of biomimetic nanoparticles. Biomaterials 82, 168–177 (2016). https://doi.org/10.1016/j.biomaterials.2015.11.054
F. Cattaneo, M. Parisi, T. Fioretti, D. Sarnataro, G. Esposito, R. Ammendola, Nuclear localization of formyl-peptide receptor 2 in human cancer cells. Arch Biochem Biophys 603, 10–19 (2016). https://doi.org/10.1016/j.abb.2016.05.006
M. Caterino, A. Pastore, M.G. Strozziero, G. Di Giovamberardino, E. Imperlini, E. Scolamiero, L. Ingenito, S. Boenzi, F. Ceravolo, D. Martinelli, C. Dionisi-Vici, M. Ruoppolo, The proteome of cblC defect: In vivo elucidation of altered cellular pathways in humans. J Inherited Metab Dis 38, 969–979 (2015). https://doi.org/10.1007/s10545-014-9806-4
S. Spaziani, E. Imperlini, A. Mancini, M. Caterino, P. Buono, S. Orru, Insulin-like growth factor 1 receptor signaling induced by supraphysiological doses of IGF-1 in human peripheral blood lymphocytes. Proteomics 14, 1623–1629 (2014). https://doi.org/10.1002/pmic.201300318
M. Costanzo, A. Cevenini, E. Marchese, E. Imperlini, M. Raia, L. Del Vecchio, M. Caterino and M. Ruoppolo, Label-Free Quantitative Proteomics in a Methylmalonyl-CoA Mutase-Silenced Neuroblastoma Cell Line. Int J Mol Sci 19, (2018). doi: ijms19113580
Y. Aghazadeh, V. Papadopoulos, The role of the 14-3-3 protein family in health, disease, and drug development. Drug Discov Today 21, 278–287 (2016). https://doi.org/10.1016/j.drudis.2015.09.012
J.R. Lakowicz, Principles of Fluorescence Spectroscopy, (Springer US, 2006)
M. Tramier, M. Zahid, J.C. Mevel, M.J. Masse, M. Coppey-Moisan, Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells. Microsc Res Tech 69, 933–939 (2006). https://doi.org/10.1002/jemt.20370
A. Margineanu, J.J. Chan, D.J. Kelly, S.C. Warren, D. Flatters, S. Kumar, M. Katan, C.W. Dunsby and P.M.W. French, Screening for protein-protein interactions using Forster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) (vol 6, 28186, 2016). Sci Rep 6, (2016). https://doi.org/10.1038/srep28186
B.J. Bacskai, J. Skoch, G.A. Hickey, R. Allen, B.T. Hyman, Fluorescence resonance energy transfer determinations using multiphoton fluorescence lifetime imaging microscopy to characterize amyloid-beta plaques. J Biomed Opt 8, 368–375 (2003). https://doi.org/10.1117/1.1584442
S.A. Beausoleil, M. Jedrychowski, D. Schwartz, J.E. Elias, J. Villen, J.X. Li, M.A. Cohn, L.C. Cantley, S.P. Gygi, Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130–12135 (2004). https://doi.org/10.1073/pnas.0404720101
H. Daub, J.V. Olsen, M. Bairlein, F. Gnad, F.S. Oppermann, R. Korner, Z. Greff, G. Keri, O. Stemmann, M. Mann, Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438–448 (2008). https://doi.org/10.1016/j.molcel.2008.07.007
J.V. Olsen, M. Vermeulen, A. Santamaria, C. Kumar, M.L. Miller, L.J. Jensen, F. Gnad, J. Cox, T.S. Jensen, E.A. Nigg, S. Brunak and M. Mann, Quantitative Phosphoproteomics Reveals Widespread Full Phosphorylation Site Occupancy During Mitosis. Science Signaling 3, ra3 (2010). https://doi.org/10.1126/scisignal.2000475
C. Johnson, S. Crowther, M.J. Stafford, D.G. Campbell, R. Toth, C. MacKintosh, Bioinformatic and experimental survey of 14-3-3-binding sites. Biochem J 427, 69–78 (2010). https://doi.org/10.1042/Bj20091834
P.M. Chan, Y.W. Ng, E. Manser, A robust protocol to map binding sites of the 14-3-3 Interactome: Cdc25C requires phosphorylation of both S216 and S263 to bind 14-3-3. Mol Cell Proteomics 10, M110.005157 (2011). https://doi.org/10.1074/mcp.M110.005157
A. Gessner, M. Thomas, P.G. Castro, L. Buchler, A. Scholz, T.H. Brummendorf, N.M. Soria, J. Vormoor, J. Greil, O. Heidenreich, Leukemic fusion genes MLL/AF4 and AML1/MTG8 support leukemic self-renewal by controlling expression of the telomerase subunit TERT. Leukemia 24, 1751–1759 (2010). https://doi.org/10.1038/leu.2010.155
M.G. Guenther, L.N. Lawton, T. Rozovskaia, G.M. Frampton, S.S. Levine, T.L. Volkert, C.M. Croce, T. Nakamura, E. Canaani, R.A. Young, Aberrant chromatin at genes encoding stem cell regulators in human mixed-lineage leukemia. Genes Dev 22, 3403–3408 (2008). https://doi.org/10.1101/gad.1741408
H. Okuda, A. Kanai, S. Ito, H. Matsui, A. Yokoyama, AF4 uses the SL1 components of RNAP1 machinery to initiate MLL fusion- and AEP-dependent transcription. Nat Commun 6, 8869 (2015). https://doi.org/10.1038/Ncomms9869
H. Okuda, S. Takahashi, A. Takaori-Kondo, A. Yokoyama, TBP loading by AF4 through SL1 is the major rate-limiting step in MLL fusion-dependent transcription. Cell Cycle 15, 2712–2722 (2016). https://doi.org/10.1080/15384101.2016.1222337
S.Q. Pan, P.C. Sehnke, R.J. Ferl, W.B. Gurley, Specific interactions with TBP and TFIIB in vitro suggest that 14-3-3 proteins may participate in the regulation of transcription when part of a DNA binding complex. Plant Cell 11, 1591–1602 (1999). https://doi.org/10.1105/tpc.11.8.1591
Acknowledgements
We are grateful to Jean Ann Gilder (Scientific Communication srl, Naples, Italy) for revising and editing the text and Vittorio Lucignano, CEINGE-Biotecnologie Avanzate, for technical assistance. This work was supported by Italian Ministry of Health [RF-2011-02349269 to GE]. We are also grateful to AIL Onlus for supporting research in the field of leukemias. Tiziana Fioretti and Armando Cevenini performed and supervised the experimental analyses, evaluated the overall data and drafted the manuscript. Mariateresa Zanobio, Maddalena Raia and Daniela Sarnataro contributed to experimental and data analyses. Francesco Salvatore supervised the entire study and performed a critical review of the manuscript. Gabriella Esposito designed, coordinated and supervised the entire study, and contributed to draft and revise the manuscript.
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Fioretti, T., Cevenini, A., Zanobio, M. et al. Crosstalk between 14-3-3θ and AF4 enhances MLL-AF4 activity and promotes leukemia cell proliferation. Cell Oncol. 42, 829–845 (2019). https://doi.org/10.1007/s13402-019-00468-6
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DOI: https://doi.org/10.1007/s13402-019-00468-6