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Diagnostic and prognostic relevance of salivary microRNA-21, -125a, -31 and -200a levels in patients with oral lichen planus - a short report

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Abstract

Background

Oral lichen planus (OLP), a relatively common chronic inflammatory disease of the oral mucosa, is considered to be a premalignant disorder of the oral cavity. Previously, several biomarkers have been tested for their diagnostic potential. Here, we aimed to investigate the diagnostic potential of four miRNAs, miR-21, -125a, -31 and -200a, known to be involved in oral squamous cell carcinoma (OSCC) development, in the saliva of OLP patients as also their putative relation to OSCC development in these patients.

Materials and methods

Saliva samples from 30 patients with OLP were collected, 15 of whom were diagnosed with dysplasia upon histopathologic examination. In addition, 15 saliva samples from patients with OSCC and 15 saliva samples from healthy donors were collected. After RNA extraction, the respective miRNA levels were assessed by quantitative RT-PCR.

Results

We found that the miR-21 levels were significantly increased in saliva samples derived from patients with OLP, dysplastic OLP and OSCC, compared to those from healthy controls (p = 0.012, p = 0.0017 and p < 0.0001, respectively). Conversely, significant decreases in miR-125a levels were found in the OLP, dysplastic OLP and OSCC samples, compared to those from healthy controls (p < 0.0014, p < 0.0001 and p < 0.0001, respectively). In addition, significant increases in miR-31 levels were found in samples derived from dysplastic OLP and OSCC patients, but not in those from nondysplastic OLP patients, compared to those in healthy controls (p = 0.01 and p = 0.004, respectively). Finally, we found that the miR-200a levels were significantly decreased only in samples derived from OSCC patients (p < 0.0001).

Conclusions

From our data we conclude that increased miR-21 levels in conjunction with decreased miR-125a levels in saliva of OLP patients may be indicative for a poor prognosis. Conversely, we conclude that lack of significant alterations in miR-31 and miR-200a levels in saliva of OLP patients may be indicative for absence of malignant transformation.

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Fig. 1: Comparative salivary miR-200a, miR-21, miR-31 and miR-125a levels.
Fig. 2: Histograms showing relative miRNA levels.

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Author information

Authors and Affiliations

  1. Department of Oral Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Masoumeh Mehdipour, Soudeh Jafari & Hamed Mortazavi

  2. Hematology and blood banking Department, Faculty of Allied Medicine & Cellular & Molecular Research Center (CMRC), Iran University of Medical Sciences, Tehran, Iran

    Minoo Shahidi

  3. Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

    Soheila Manifar

  4. Department of Oral Pathology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Fatemeh Mashhadi Abbas

  5. Department of Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mahmood Barati

  6. Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

    Mohammad Shirkhoda

  7. Research Center for Prevention of Oral and Dental Diseases, Baghiyatallah, University of Medical Sciences, Tehran, Iran

    Amir Farzanegan

  8. Department of Oral and Maxillofacial Medicine, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran

    Zahra Elmi Rankohi

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  1. Masoumeh Mehdipour
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Correspondence to Soudeh Jafari.

Electronic supplementary material

Supplementary Fig. 1

Oral lesions: The illustrations show the oral lesions in a patient with OLP, erosive and ulcerative type (a, b &c) and a patient with OSCC (d & e). (TIFF 1164 kb)

High resolution image (GIF 605 kb)

Supplementary Fig. 2

Representative histopathologic micrographs: Different histopathologic sections (hematoxylin-eosin) including nondysplastic OLP (a), Dysplastic OLP (b), along with OSCC (c) by 40× magnification, as reported by two independent pathologists (OLP = Oral Lichen Planus & OSCC = squamous cell carcinoma (TIFF 966 kb)

High resolution image (GIF 552 kb)

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Mehdipour, M., Shahidi, M., Manifar, S. et al. Diagnostic and prognostic relevance of salivary microRNA-21, -125a, -31 and -200a levels in patients with oral lichen planus - a short report. Cell Oncol. 41, 329–334 (2018). https://doi.org/10.1007/s13402-018-0372-x

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