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Gene copy number variation in male breast cancer by aCGH

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An Erratum to this article was published on 01 October 2011

Abstract

Background

Male breast cancer (MBC) is a rare disease and little is known about its etiopathogenesis. Array comparative genomic hybridization (aCGH) provides a method to quantitatively measure the changes of DNA copy number and to map them directly onto the complete linear genome sequences. The aim of this study was to investigate DNA imbalances by aCGH and compare them with a female breast cancer dataset.

Methods

We used Agilent Human Genome CGH Microarray Kit 44B and 44 K to compare genomic alterations in 25 male breast cancer tissues studied at NCC of Bari and 16 female breast cancer deposited with the Gene Expression Omnibus (GSE 12659). Data analysis was performed with Nexus Copy Number 5.0 software.

Results

All the 25 male and 16 female breast cancer samples displayed some chromosomal instability (110,93 alterations per patient in female, 69 in male). However, male samples presented a lower frequency of genetic alterations both in terms of loss and gains.

Conclusion

aCGH is an effective tool for analysis of cytogenetic aberrations in MBC, which involves different biological processes than female. Male most significant altered regions contained genes involved in cell communication, cell division and immunological response, while female cell-cell junction maintenance, regulation of transcription and neuron development.

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Authors and Affiliations

  1. Clinical Experimental Oncology Laboratory, National Cancer Centre of Bari, viale Orazio Flacco 65, 70124, Bari, Italy

    Stefania Tommasi, Anita Mangia, Giuseppina Iannelli, Patrizia Chiarappa & Angelo Paradiso

  2. Department of General Biology and Medical Genetics, University of Pavia, Pavia, Italy

    Elena Rossi & Orsetta Zuffardi

  3. Department of Experimental Medicine, University La Sapienza, Rome, Italy

    Laura Ottini

  4. Division of Medical Oncology A, Regina Elena Cancer Institute, Rome, Italy

    Marcella Mottolese

  5. Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori, Meldola, FC, Italy

    Wainer Zoli

Authors
  1. Stefania Tommasi
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  2. Anita Mangia
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  3. Giuseppina Iannelli
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  4. Patrizia Chiarappa
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Corresponding author

Correspondence to Anita Mangia.

Additional information

Stefania Tommasi and Anita Mangia equally contributed to this work.

This paper is a reprint from ‘Gene copy number variation in male breast cancer by aCGH, Stefania Tommasi, Anita Mangia, Giuseppina Iannelli, Patrizia Chiarappa, Elena Rossi, Laura Ottini, Marcella Mottolese, Wainer Zoli, Orsetta Zuffardi and Angelo Paradiso’ originally published in Analytical Cellular Pathology/Cellular Oncology, Volume 33, number 3–4, 2010, pp. 113–119, IOS Press.

An erratum to this article can be found at http://dx.doi.org/10.1007/s13402-011-0063-3

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Tommasi, S., Mangia, A., Iannelli, G. et al. Gene copy number variation in male breast cancer by aCGH. Cell Oncol. 34, 467–473 (2011). https://doi.org/10.1007/s13402-011-0041-9

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