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Comparative Genomic Hybridization and Array Based CGH in Cancer

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Molecular Testing in Cancer

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Abstract

Genomic instability is a key feature of cancer. Chromosomal rearrangements and segmental DNA copy-number alterations in the tumor genome lead to aberrant gene expression pattern. Array-based comparative genomic hybridization (aCGH) is a molecular cytogenetic technique designed to measure DNA copy-number status and identify segmental dosage alterations. The emergence of aCGH technology is placed into context in the evolution of molecular cytogenetics. The defining characteristics of contemporary genomic array platforms are reviewed, and the material considerations for using these platforms are explained. This chapter describes the development and current knowledge on aCGH technology and its role in cancer research and clinical diagnostics.

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    Authors and Affiliations

    1. Department of Integrative Oncology, BC Cancer Agency and Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada

      Roland Hubaux Ph.D., Victor D. Martinez Ph.D., David Rowbotham B.SC. & Wan L. Lam Ph.D.

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    Correspondence to Victor D. Martinez Ph.D. .

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    1. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

      George M. Yousef

    2. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada

      Serge Jothy

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    Hubaux, R., Martinez, V.D., Rowbotham, D., Lam, W.L. (2014). Comparative Genomic Hybridization and Array Based CGH in Cancer. In: Yousef, G., Jothy, S. (eds) Molecular Testing in Cancer. Springer, New York, NY. https://doi.org/10.1007/978-1-4899-8050-2_3

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