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Establishment and genetic characterization of an immortal tumor cell line derived from intestinal-type sinonasal adenocarcinoma

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Abstract

Background

Intestinal-type sinonasal adenocarcinoma (ITAC) is a rare tumor etiologically related to professional exposure to wood dust. The overall prognosis is poor, mainly due to the difficulty to resect the tumor completely in this anatomically complex region. Therefore, there is great need for alternative treatments. However, the lack of a good tumor model system for ITAC has hampered the development and testing of new therapeutic agents. Here, we report the establishment and characterization of the first human ITAC cell line named ITAC-3.

Methods

The cell line was initiated from small explants of a T4bN0M0 colonic type ITAC from the ethmoid sinus. Growth and invasion parameters as well as genetic characteristics were analyzed.

Results

The population doubling time was 18 h and the cell line was capable of invasion in matrigel. Chromosomal analysis showed a tetraploid karyotype with both numerical and structural aberrations. High resolution microarray CGH analysis identified many copy number alterations, including homozygous deletions. TP53 carried a mutation c.818G>T in exon eight concurring with a strong nuclear protein overexpression. Immunohistochemical analysis showed protein overexpression of EGFR and normal expression of β-catenin and p16.

Conclusion

This is the first report of the establishment of a cell line derived from a primary ITAC. The genomic profile of the cell line was the same as the primary tumor from which it was derived. This new cell line will be a useful tool for the development and testing of new therapeutic agents for this tumor type.

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Acknowledgements

This work was supported by grants PI05-1387, PI08-1599 and EMER07-048 of Fondos de Investigación Sanitaria (FIS) and RD06/0020/0034 of Red Temática de Investigación Cooperativa en Cáncer (RTICC), Spain. JPE was supported by the Spanish Ministry of Science and Innovation (MICINN), co financed by the European Social Fund.

Conflict of interest statement

None declared.

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Authors and Affiliations

  1. Department of Otolaryngology, IUOPA, Hospital Universitario Central de Asturias, Oviedo, Asturias, Spain

    Jhudit Pérez-Escuredo, Jorge García Martínez, Cristina García-Inclán, María Costales, César Álvarez Marcos & José Luis Llorente

  2. Department Pathology, Hospital Universitario Central de Asturias, Oviedo, Asturias, Spain

    Blanca Vivanco

  3. Department of Otolaryngology, Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Edificio H Covadonga 1ª Planta Centro, lab 2, Hospital Universitario Central de Asturias, Celestino Villamil s/n, 33006, Oviedo, Asturias, Spain

    Mario A. Hermsen

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  1. Jhudit Pérez-Escuredo
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Correspondence to Mario A. Hermsen.

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Pérez-Escuredo, J., García Martínez, J., García-Inclán, C. et al. Establishment and genetic characterization of an immortal tumor cell line derived from intestinal-type sinonasal adenocarcinoma. Cellular Onc. 34, 23–31 (2011). https://doi.org/10.1007/s13402-010-0002-8

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