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Isolation and screening of Tetradesmus dimorphus and Desmodesmus asymmetricus from natural habitats in Northwestern China for clean fuel production and N, P removal

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Abstract

Isolation and screening of microalgal species from natural habitats should be a continues work to discover effective species for biofuel generation and wastewater treatment. In the current study, microalgal species namely Tetradesmus dimorphus GEEL-04 and Desmodesmus asymmetricus GEEL-05 were isolated, identified, and assessed for growth kinetics, nutrients removal efficiency, and whole biomass composition. T. dimorphus showed higher growth (1.46 OD680nm) compared with D. asymmetricus (1.16 OD680nm), and both showed 99% total nitrogen and total phosphorous removal. T. dimorphus and D. asymmetricus accumulated higher protein content (40.02 and 45.10%), carbohydrate (31.91 and 24.64%), and lipid (15.41 and 24.16%), respectively. The FTIR spectra absorbance revealed different function groups in microalgal biomass available for biofuel generation. Elemental analysis of microalgal biomass showed high carbon content 50.13% and 43.01% for T. dimorphus and D. asymmetricus, respectively. Thermogravimetric analysis indicated ≥ 80% volatile content in both isolates. The fatty acid composition of both microalgal strains showed high palmitic acid and linoleic acid corresponding to high quality biodiesel. These results indicated that the T. dimorphus and D. asymmetricus could be potential candidates for high biocomponents accumulation and N, P removal.

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Funding

This study was supported by a start-up fund for the “Construction of the double first-class” project (grant number 561119201).

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Authors and Affiliations

  1. Department of Occupational and Environmental Health, School of Public Health, Lanzhou University, Lanzhou, 730000, Gansu Province, People’s Republic of China

    Le Wang, Lei Wang, Habasi Patrick Manzi, Qi Yang, Zhaodi Guo, Xingrong Liu & El-Sayed Salama

  2. Department of Molecular Biology, School of Medicine Biochemistry, Indiana University, Indianapolis, IN, 46202, USA

    Yuanzhang Zheng

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  1. Le Wang
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Correspondence to Xingrong Liu or El-Sayed Salama.

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Wang, L., Wang, L., Manzi, H.P. et al. Isolation and screening of Tetradesmus dimorphus and Desmodesmus asymmetricus from natural habitats in Northwestern China for clean fuel production and N, P removal. Biomass Conv. Bioref. 12, 1503–1512 (2022). https://doi.org/10.1007/s13399-020-01034-z

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