Abstract
Particles were ablated from laser desorption and inlet ionization matrix thin films with a UV laser in reflection and transmission geometries. Particle size distributions were measured with a combined scanning mobility particle sizer (SMPS) and aerodynamic particle sizer (APS) system that measured particles in the size range from 10 nm to 20 μm. The matrixes investigated were 2,5-dihydroxybenzoic acid (DHB), α-cyano-4-hydroxycinnamic acid (CHCA), sinapic acid (SA), 2,5-dihydroxy-acetophenone (DHAP), and 2-nitrophloroglucinol (NPG). Nanoparticles with average diameters between 20 and 120 nm were observed in both transmission and reflection geometry. The particle mass distribution was significantly different in reflection and transmission geometry. In reflection geometry, approximately equal mass was distributed between particles in the 20 to 450 nm range of diameters and particles in the 450 nm to 1.5 μm diameter range. In transmission mode, the particle mass distribution was dominated by large particles in the 2 to 20 μm diameter range. Ablation of inlet ionization matrices DHAP and NPG produced particles that were 3 to 4 times smaller compared with the other matrices. The results are consistent with ion formation by nanoparticle melting and breakup or melting and breakup of the large particles through contact with heated inlet surfaces.
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Trimpin, S., Inutan, E.D., Herath, T.N., McEwen, C.N.: Laserspray ionization, a new atmospheric pressure MALDI method for producing highly charged gas-phase ions of peptides and proteins directly from solid solutions. Mol. Cel. Proteom. 9, 362–367 (2010)
Trimpin, S., Inutan, E.D., Herath, T.N., McEwen, C.N.: Matrix-assisted laser desorption/ionization mass spectrometry method for selectively producing either singly or multiply charged molecular ions. Anal. Chem. 82, 11–15 (2010)
Inutan, E.D., Wang, B., Trimpin, S.: Commercial Intermediate Pressure MALDI Ion mobility spectrometry mass spectrometer capable of producing highly charged laserspray ionization ions. Anal. Chem. 83, 678–684 (2011)
Trimpin, S., Ren, Y., Wang, B., Lietz, C.B., Richards, A.L., Marshall, D.D., Inutan, E.D.: Extending the laserspray ionization concept to produce highly charged ions at high vacuum on a time-of-flight mass analyzer. Anal. Chem. 83, 5469–5475 (2011)
Overberg, A., Karas, M., Bahr, U., Kaufmann, S., Hillenkamp, F.: Matrix-assisted infrared-laser (2.94 mm) desorption/ionization mass spectrometry of large biomolecules. Rapid Commun. Mass Spectrom 4, 293–296 (1990)
Overberg, A., Karas, M., Hillenkamp, F.: Matrix-assisted laser desorption of large biomolecules with a TEA-CO2-Laser. Rapid Commun. Mass Spectrom. 5, 128–131 (1991)
Menzel, C., Dreisewerd, K., Berkenkamp, S., Hillenkamp, F.: The role of the laser pulse duration in infrared matrix-assisted laser desorption/ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 13, 975–984 (2002)
McEwen, C.N., Trimpin, S.: An alternative ionization paradigm for atmospheric pressure mass spectrometry: Flying elephants from Trojan horses. Int. J. Mass Spectrom. 300, 167–172 (2011)
Zilch, L.W., Maze, J.T., Smith, J.W., Ewing, G.E., Jarrold, M.F.: Charge separation in the aerodynamic breakup of micrometer-sized water droplets. J. Phys. Chem. A 112, 13352–13363 (2008)
Musapelo, T., Murray, K.K.: Particle formation in ambient MALDI Plumes. Anal. Chem 83, 6601–6608 (2011)
Alves, S., Kalberer, M., Zenobi, R.: Direct detection of particles formed by laser ablation of matrices during matrix-assisted laser desorption/ionization. Rapid. Commun. Mass. Spectrom. 17, 2034–2038 (2003)
Jackson, S.N., Mishra, S., Murray, K.K.: Characterization of coarse particles formed by laser ablation of MALDI matrixes. J. Phys. Chem. B 107, 13106–13110 (2003)
Kim, J.-K., Jackson, S.N., Murray, K.K.: Matrix-assisted laser desorption/ionization mass spectrometry of collected bioaerosol particles. Rapid Commun. Mass Spectrom. 19, 1725–1729 (2005)
Fan, X., Little, M.W., Murray, K.K.: Infrared laser wavelength dependence of particles ablated from glycerol. Appl. Surf. Sci 255, 1699–1704 (2008)
Li, J., Inutan, E.D., Wang, B., Lietz, C.B., Green, D.R., Manly, C.D., Richards, A.L., Marshall, D.D., Lingenfelter, S., Ren, Y., Trimpin, S.: Matrix assisted ionization: New aromatic and nonaromatic matrix compounds producing multiply charged lipid, peptide, and protein ions in the positive and negative mode observed directly from surfaces. J. Am. Soc. Mass Spectrom. 23, 1625–1643 (2012)
Zhigilei, L.V., Yingling, Y.G., Itina, T.E., Schoolcraft, T.A., Garrison, B.J.: Molecular dynamics simulations of matrix-assisted laser desorption-connections to experiment. Int. J. Mass. Spectrom. 226, 85–106 (2003)
Miotello, A., Kelly, R.: Laser-induced phase explosion: New physical problems when a condensed phase approaches the thermodynamic critical temperature. Appl. Phys. A 69, S67–S73 (1999)
Zhigilei, L.V., Garrison, B.J.: Microscopic mechanisms of laser ablation of organic solids in the thermal and stress confinement irradiation regimes. J. Appl. Phys. 88, 1281–1298 (2000)
Apitz, I., Vogel, A.: Material ejection in nanosecond Er:YAG laser ablation of water, liver, and skin. Appl. Phys. A 81, 329–338 (2005)
Vogel, A., Venugopalan, V.: Mechanisms of pulsed laser ablation of biological tissues. Chem. Rev. 103, 577–644 (2003)
Knochenmuss, R., Zhigilei, L.V.: Molecular dynamics model of ultraviolet matrix-assisted laser desorption/ionization including ionization processes. J. Phys. Chem. B 109, 22947–22957 (2005)
Leisner, A., Rohlfing, A., Rohling, U., Dreisewerd, K., Hillenkamp, F.: Time-resolved imaging of the plume dynamics in infrared matrix-assisted laser desorption/ionization with a glycerol matrix. J. Phys. Chem. B 109, 11661–11666 (2005)
Zhigilei, L.V., Ivanov, D.S., Leveugle, E., Sadigh, B., Bringa, E.M.: Computer simulations of laser ablation from simple metals to complex metallic alloys. Proceedings of SPIE 5448, 505–519 (2004)
Nikolayev, V.S., Beysens, D.A.: Boiling crisis and non-equilibrium drying transition. Europhys. Lett. 47, 345–351 (1999)
Bulgakova, N.M., Bulgakov, A.V.: Pulsed laser ablation of solids: Transition from normal vaporization to phase explosion. Appl. Phys. A 73, 199–208 (2001)
Galicia, M., Vertes, A., Callahan, J.: Atmospheric pressure matrix-assisted laser desorption/ionization in transmission geometry. Anal. Chem. 74, 1891–1895 (2002)
Trimpin, S., Wang, B., Inutan, E.D., Li, J., Lietz, C.B., Harron, A., Pagnotti, V.S., Sardelis, D., McEwen, C.N.: A mechanism for ionization of nonvolatile compounds in mass spectrometry: Considerations from MALDI and inlet ionization. J. Am. Soc. Mass Spectrom. 23, 1644–1660 (2012)
Frankevich, V., Nieckarz, R.J., Sagulenko, P.N., Barylyuk, K., Zenobi, R., Levitsky, L.I., Agapov, A.Y., Perlova, T.Y., Gorshkov, M.V., Tarasova, I.A.: Probing the mechanisms of ambient ionization by laser-induced fluorescence spectroscopy. Rapid Commun. Mass Spectrom. 26, 1567–1572 (2012)
Costa, A.B.; Cooks, R.G.: Simulation of atmospheric transport and droplet-thin film collisions in desorption electrospray ionization. Chem. Commun. 3915–3917. doi:10.1039/b710511h (2007)
Costa, A.B., Cooks, R.G.: Simulated splashes: Elucidating the mechanism of desorption electrospray ionization mass spectrometry. Chem. Phys. Lett. 464, 1–8 (2008)
Huang, F., Murray, K.K.: Finite element simulation of infrared laser ablation for mass spectrometry. Rapid Commun. Mass Spectrom. 26, 2145–2150 (2012)
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Musapelo, T., Murray, K.K. Particle Production in Reflection and Transmission Mode Laser Ablation: Implications for Laserspray Ionization. J. Am. Soc. Mass Spectrom. 24, 1108–1115 (2013). https://doi.org/10.1007/s13361-013-0631-z
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DOI: https://doi.org/10.1007/s13361-013-0631-z