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Comparison of MS/MS Methods for Characterization of DNA/Cisplatin Adducts

  • Research Article
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Journal of The American Society for Mass Spectrometry

Abstract

The development of activation/dissociation techniques such as ultraviolet photodissociation (UVPD), infrared multiphoton dissociation (IRMPD), and electron transfer dissociation (ETD) as alternatives to collision induced dissociation (CID) has extended the range of strategies for characterizing biologically relevant molecules. Here, we describe a comprehensive comparison of CID, IRMPD, UVPD, ETD, and hybrid processes termed ETcaD and ET-IRMPD (and analogous hybrid methods in the negative mode NETcaD and NET-IRMPD) for generating sequence-specific fragment ions and allowing adduction sites to be pinpointed for DNA/cisplatin adducts. Among the six MS/MS methods, the numerous products generated by the IRMPD and UVPD techniques resulted in the most specific and extensive backbone cleavages. We conclude that IRMPD and UVPD methods generally offer the best characteristics for pinpointing the cisplatin adduction sites in the fragment-rich spectra.

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Acknowledgments

The authors gratefully acknowledge funding from NIH (RO1 GM65956) and the Welch Foundation (F1155).

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  1. Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, TX, 78712, USA

    Zhe Xu, Jared B. Shaw & Jennifer S. Brodbelt

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  1. Zhe Xu
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  2. Jared B. Shaw
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  3. Jennifer S. Brodbelt
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Correspondence to Jennifer S. Brodbelt.

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Xu, Z., Shaw, J.B. & Brodbelt, J.S. Comparison of MS/MS Methods for Characterization of DNA/Cisplatin Adducts. J. Am. Soc. Mass Spectrom. 24, 265–273 (2013). https://doi.org/10.1007/s13361-012-0532-6

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