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Susceptibility of oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) pupae to entomopathogenic fungi

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Abstract

Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is considered as a highly invasive and destructive agricultural pest due to its strong dispersal and adaptive capacity. We evaluated the susceptibility of B. dorsalis pupae to six entomopathogenic fungi (Isaria fumosorosea IFCF01, Beauveria bassiana Bb02, B. bassiana Bb04, Metarhizium anisopliae Ma01, M. anisopliae Ma04, and M. anisopliae Ma09) in the laboratory through impregnation method (immersing fly pupae into conidial suspension). Results indicated that fungus treatment during the pupal stage of B. dorsalis, not only caused significant mortality of target pupae (4.4–14.4% mortality), but also influence the survival of the emerged adults (38.9–72.8% mortality on the 10 days post-emergence). Among the six entomopathogenic fungi, M. anisopliae Ma04 presented the highest virulence against B. dorsalis. Results of virulence bioassay indicated that the LC50 values of M. anisopliae Ma04 against B. dorsalis declined from 5.2 × 1028 to 5.2 × 107 conidia/ml over a 1–10-day period post adult emergence, and the LT50 values decreased from 5.25 to 2.78 days with the concentrations of conidial suspension increasing from 1.0 × 108 to 1.0 × 1010 conidia/ml. Therefore, M. anisopliae Ma04 have a greater potential for B. dorsalis control.

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Acknowledgements

This study was supported by the National Key Research and Development Program of China (2018YFD0201300), and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (2019KJ125).

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Author notes

  1. Desen Wang, Qiling Liang and Minzhong Chen contributed equally to this study.

Authors and Affiliations

  1. College of Plant Protection, South China Agricultural University, Guangzhou, 510642, China

    Desen Wang, Qiling Liang, Hui Ye, Junyi Yin & Yurong He

  2. Key Laboratory of Bio-Pesticide Innovation and Application, Guangzhou, 510642, Guangdong, China

    Desen Wang & Yurong He

  3. Engineering Research Center of Biological Control, Ministry of Education, Guangzhou, 510642, China

    Desen Wang & Yurong He

  4. Zhanjiang Agricultural Technology Extension Center, Zhanjiang, 524000, China

    Minzhong Chen

  5. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Yonglin Liao, Lihua Lü, Yanyuan Lei & Waqar Jaleel

  6. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, 510640, China

    Yonglin Liao

  7. National Nature Reserve Administration of Henan Jigong Mountain, Xinyang, 464000, China

    Dongzhang Cai

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  1. Desen Wang
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Correspondence to Yurong He.

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Wang, D., Liang, Q., Chen, M. et al. Susceptibility of oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) pupae to entomopathogenic fungi. Appl Entomol Zool 56, 269–275 (2021). https://doi.org/10.1007/s13355-021-00734-w

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