Abstract
The leaf rust resistance gene Lr25, transferred from Secale cereale L. into wheat and located on chromosome 4B, imparts resistance to all pathotypes of leaf rust in South-East Asia. In an F2-derived F3 population, created by crossing TcLr25 that carries the gene Lr25 for leaf rust resistance with leaf rust-susceptible parent Agra Local, three microsatellite markers located on the long arm of chromosome 4B were found to be linked to the Lr25 locus. The donor parent TcLr25 is a near-isogenic line derived from the variety Thatcher. The most virulent pathotype of leaf rust in the South-East Asian region, designated 77–5 (121R63-1), was used for challenging the population under artificially controlled conditions. The marker Xgwm251 behaved as a co-dominant marker placed 3.8 cM away from the Lr25 locus on 4BL. Two null allele markers, Xgwm538 and Xgwm6, in the same linkage group were located at a distance of 3.8 cM and 16.2 cM from the Lr25 locus, respectively. The genetic sequence of Xgwm251, Lr25, Xgwm538, and Xgwm6 covered a total length of 20 cM on 4BL. The markers were validated for their specificity to Lr25 resistance in a set of 43 wheat genetic stocks representing 43 other Lr genes.
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Bhardwaj SC, Prashar M, Jain SK, Kumar S, Sharma YP, Sivasamy M, Kalappanavar IK (2010) Virulence of Puccinia triticina on Lr28 in wheat and its evolutionary relation to prevalent pathotypes in India. Cereal Res Commun 38(1):83–88
Cheng FS, Weeden NF, Brown SK, Aldwinckle HS, Gardiner SE, Bus VG (1998) Development of a DNA marker for Vm, a gene conferring resistance to apple scab. Genome 41:208–214
Driscoll CJ, Anderson LM (1967) Cytogenetic studies of Transec—a wheat–rye translocation line. Can J Genet Cytol 9:375–380
Driscoll CJ, Bielig LM (1968) Mapping of the Transec wheat–rye translocation. Can J Genet Cytol 10:421–425
Driscoll CJ, Jensen NF (1965) Release of a wheat–rye translocation stock involving leaf rust and powdery mildew resistances. Crop Sci 5:279–280
Friebe B, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87
Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breeding 118:369–390
Gupta SK, Charpe A, Prabhu KV, Haque QMR (2006) Identification and validation of molecular markers linked to the leaf rust resistance gene Lr19 in wheat. Theor Appl Genet 113:1027–1036
Huerta-Espino J, Roelfs AP (1992) Leaf rust on durum wheats. Vortr Pflanzenzuchtg 24:100–102
Huszar J, Bartos P, Hanzalova A (2001) Importance of wheat disease resistance for sustainable agriculture. Acta Fytotech Zootech 4:292–294
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Long DL, Roelfs AP, Leonard KJ, Roberts JJ (1993) Virulence and diversity of Puccinia recondita f. sp. tritici in the United States in 1991. Plant Dis 77:786–791
McCallum BD, Seto-Goh P (2002) Physiologic specialization of wheat leaf rust (Puccinia triticina) in Canada in 1999. Can J Plant Pathol 24:205–210
McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO Publications, Victoria, Australia, 200 pp
McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalog of gene symbols for wheat. Proceedings of the 11th International Wheat Genetics Symposium. Sydney University Press, Sydney, pp 143–150
Melchinger AE (1990) Use of molecular markers in breeding for oligogenic disease resistance. Plant Breeding 104:1–19
Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9829–9832
Nayar SK, Nagarajan S, Bahadur P (1985) Results of Puccinia recondita f.sp. tritici virulence monitoring survey in India—1981 & 1982. Indian Phytopathol 33:252–257
Prabhu KV, Somers DJ, Rakow G, Gugel RK (1998) Molecular markers linked to white rust resistance in mustard Brassica juncea. Theor Appl Genet 97:865–870
Procunier JD, Townley-Smith TF, Fox S, Prashar S, Gray M, Kim WK, Czarnecki E, Dyck PL (1995) PCR-based RAPD/DGGE markers linked to leaf rust resistance genes Lr29 and Lr25 in wheat (Triticum aestivum L.). J Genet Breed 49:87–92
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Roelfs AP (1988) Resistance to leaf and stem rusts in wheat. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico DF, pp 10–22
Samsampour D, Maleki Zanjani B, Singh A, Pallavi JK, Prabhu KV (2009) Marker assisted selection to pyramid seedling resistance gene Lr24 and adult plant resistance gene Lr48 for leaf rust resistance in wheat. Indian J Genet 69:1–9
Samsampour D, Maleki Zanjani B, Pallavi JK, Singh A, Charpe A, Gupta SK, Prabhu KV (2010) Identification of molecular markers linked to adult plant leaf rust resistance gene Lr48 in wheat and detection of Lr48 in the Thatcher near-isogenic line with gene Lr25. Euphytica 174:337–342
Schachermayr GM, Messmer MM, Feuillet C, Winzeler H, Winzeler M, Keller B (1995) Identification of molecular markers linked to the Agropyron elongatum-derived leaf rust resistance gene Lr24 in wheat. Theor Appl Genet 90:982–990
Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Sci 43:333–336
Stakman EC, Stewart DM, Loegering WQ (1962) Identification of physiological races of Puccinia graminis var tritici. Agricultural Research Service E617. United States Department of Agriculture, Washington DC, p 53
Timmerman GM, Frew TJ, Weeden NF, Miller AL, Goulden DS (1994) Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.). Theor Appl Genet 88:1050–1055
Tomar SMS, Menon MK (1998) Adult plant response of near-isogenic lines and stocks of wheat carrying specific Lr genes against leaf rust. Indian Phytopathol 51:61–67
Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Res 14:415–421
Acknowledgements
The authors are grateful to the Indian Council of Agricultural Research for sponsoring the project and funding the fellowship to AS and JKP under the Molecular Breeding Network Project. We acknowledge Dr. R. G. Saini for supplying the parental material of the Lr gene donors. The authors are grateful to the Head, Regional Station, Indian Agricultural Research Institute, Wellington, for providing the pure seed of the near-isogenic lines of wheat and the Directorate of Wheat Research, Flowerdale, Shimla, for providing the pure inoculum of leaf rust pathogen.
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Singh, A., Pallavi, J.K., Gupta, P. et al. Identification of microsatellite markers linked to leaf rust resistance gene Lr25 in wheat. J Appl Genetics 53, 19–25 (2012). https://doi.org/10.1007/s13353-011-0070-0
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DOI: https://doi.org/10.1007/s13353-011-0070-0