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Development and validation of a user-friendly KASP marker for the Sw-5 locus in tomato

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Abstract

Tomato spotted wilt virus (TSWV) is a serious pathogen of tomato. The most efficient strategy for control of TSWV is the breeding of varieties resistant to the virus. In tomatoes, the Sw-5 locus controls resistance to TSWV. Molecular markers based on single nucleotide polymorphisms (SNPs) are widely used for screening in plant breeding. In the present study, we developed primers specific to the Sw-5 locus for use in Kompetitive Allele-Specific PCR (KASP) which is a novel method based on SNPs. These primers were validated in a number of tomato genotypes with different genetic backgrounds, showing that this KASP assay can be used successfully for the selection of TSWV-resistant plants in tomato breeding.

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Acknowledgements

This study was financially supported by M.Y. Genetik Tarım Teknoloji Ltd. Şti. (Antalya, Turkey). The authors would like to thank Multi Tohum Tar. San. Tic. A.Ş. (Antalya, Turkey) and Yüksel Tohum Tarım San. ve Tic. A. Ş. (Antalya, Turkey) for providing tomato materials. In addition, the authors are grateful to Dr. Atilla Göknur for his support in developing the KASP assay.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, University of Akdeniz, Antalya, Turkey

    Zübeyir Devran

  2. M.Y. Genetik Tarım Teknoloji Laboratuvar Ltd. Şti., Antalya, Turkey

    E. Kahveci

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  1. Zübeyir Devran
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  2. E. Kahveci
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Correspondence to Zübeyir Devran.

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Devran, Z., Kahveci, E. Development and validation of a user-friendly KASP marker for the Sw-5 locus in tomato. Australasian Plant Pathol. 48, 503–507 (2019). https://doi.org/10.1007/s13313-019-00651-1

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  • DOI: https://doi.org/10.1007/s13313-019-00651-1

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