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KRAS polymorphisms are associated with survival of CRC in Chinese population

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Tumor Biology

Abstract

rs12245, rs12587, rs9266, rs1137282, rs61764370, and rs712 of KRAS oncogene are characterized in the 3′UTR. The study highlights the important role of these polymorphisms playing in the susceptibility, oxaliplatin-based chemotherapy sensitivity, progression, and prognosis of CRC. Improved multiplex ligation detection reaction (iMLDR) technique is used for genotyping. An unconditional logistic regression model was used to estimate the association of certain polymorphism and CRC risk. The Kaplan–Meier method, log-rank test, and Cox regression model were used to evaluate the effects of polymorphisms on survival analysis. Results demonstrated that TT genotype and T allele of rs712 were associated with the increased risk of CRC; the patients with GG genotype and G allele of rs61764370 had a shorter survival and a higher risk of relapse or metastasis of CRC. Our studies supported the conclusions that rs61764370 and rs712 polymorphisms of the KRAS are functional and it may play an important role in the development of CRC and oxaliplatin-based chemotherapy efficiency and prognosis of CRC.

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Acknowledgments

These work successfully completed were supported by Genesky Biotechnologies, Inc. (Shanghai, China) and all the volunteer participants.

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    Authors and Affiliations

    1. Department of Human Anatomy, Luzhou Medical College, Luzhou, 646000, Sichuan, China

      Qiong Dai

    2. Department of Pathology, The First Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan, China

      Hui Lian Wei, Juan Huang, Tie Jun Zhou, Li Chai & Zhi-Hui Yang

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    1. Qiong Dai
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    2. Hui Lian Wei
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    3. Juan Huang
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    Correspondence to Zhi-Hui Yang.

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    Funding

    This work was financially supported by the State Natural Sciences Foundation Projects of China (No. 81101679), the Joint Project of Department of Technology of Luzhou, Sichuan and Luzhou Medical College (No. 2013LZLY-J44, No. 14JC0091), Fund Project of Department of Education of Sichuan Province (No. 13ZB0272), and Project of Department of Health of Sichuan Province (No. 120376).

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    Qiong Dai and Hui Lian Wei contributed equally to this work.

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    Dai, Q., Wei, H.L., Huang, J. et al. KRAS polymorphisms are associated with survival of CRC in Chinese population. Tumor Biol. 37, 4727–4734 (2016). https://doi.org/10.1007/s13277-015-4314-1

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