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Prognostic significance of human tissue kallikrein-related peptidases 11 and 15 in gastric cancer

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Tumor Biology

Abstract

Human tissue kallikrein-related peptidases (KLK) are a group of 15 serine proteases which have been investigated as potential cancer biomarkers. This study determined the prognostic significance of KLK 11 and 15 expression levels in gastric carcinoma specimens. Expression of KLK11 and KLK15 was assessed by immunohistochemistry staining on a tissue microarray constructed from 113 gastrectomy specimens from patients with gastric carcinoma. To minimize inter-observer variability, expression levels were quantified using an automated algorithm. Epithelial and stromal staining were assessed separately. Both KLK11 and KLK15 were expressed in gastric carcinoma. There was no significant correlation between either KLK11 or KLK15 expression and the presence of lymph node metastases or Lauren classification (intestinal vs. diffuse). Higher levels of KLK11 expression in gastric carcinoma were associated with significantly worse overall survival (p = 0.008), and a multivariate analysis showed that it had prognostic value independent of tumor stage and differentiation (p = 0.004). Variations in KLK15 expression were not significantly associated with prognosis. KLK11 shows promise as a potential independent prognostic marker for gastric carcinoma.

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Acknowledgments

This work was supported by grants from the Kidney Foundation of Canada, the Kidney Cancer Research Network of Canada, Prostate Cancer Canada, and the Canadian Institute of Health Research.

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Authors and Affiliations

  1. Department of Laboratory Medicine and the Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, Toronto, M5B 1W8, Canada

    David L. Kolin, Fabio Rotondo, Kalman Kovacs, Catherine J. Streutker & George M. Yousef

  2. Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, Canada

    David L. Kolin, Catherine J. Streutker & George M. Yousef

  3. Department of Pathology, Credit Valley Hospital, Mississauga, ON, L5M 2N1, Canada

    Keiyan Sy

  4. Department of Community Medicine and Public Health, Menoufiya University, Shebeen el kom, Egypt

    Mena N. Bassily

  5. Department of Medicine and Hematology-Oncology, St. Michael’s Hospital, Toronto, M5B 1W8, Canada

    Christine Brezden-Masley

  6. Department of Laboratory Medicine, St. Michael’s Hospital, 30 Bond St, Toronto, ON, M5B 1W8, Canada

    George M. Yousef

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  1. David L. Kolin
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  8. George M. Yousef
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Correspondence to George M. Yousef.

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Kolin, D.L., Sy, K., Rotondo, F. et al. Prognostic significance of human tissue kallikrein-related peptidases 11 and 15 in gastric cancer. Tumor Biol. 37, 437–446 (2016). https://doi.org/10.1007/s13277-015-3802-7

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  • DOI: https://doi.org/10.1007/s13277-015-3802-7

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