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MicroRNA-93 suppress colorectal cancer development via Wnt/β-catenin pathway downregulating

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Tumor Biology

Abstract

MicroRNA-93 (miR-93) is involved in several carcinoma progressions. It has been reported that miR-93 acts as a promoter or suppressor in different tumors. However, till now, the role of miR-93 in colon cancer is unclear. Herein, we have found that expression of miR-93 was lower in human colon cancer tissue and colorectal carcinoma cell lines compared with normal colon mucosa. Forced expression of miR-93 in colon cancer cells inhibits colon cancer invasion, migration, and proliferation. Furthermore, miR-93 may downregulate the Wnt/β-catenin pathway, which was confirmed by measuring the expression level of the β-catenin, axin, c-Myc, and cyclin-D1 in this pathway. Mothers against decapentaplegic homolog 7 (Smad7), as an essential molecular protein for nuclear accumulation of β-catenin in the canonical Wnt signaling pathway, is predicted as a putative target gene of miR-93 by the silico method and demonstrated that it may be suppressed by targeting its 3′UTR. These findings showed that miR-93 suppresses colorectal cancer development via downregulating Wnt/β-catenin, at least in part, by targeting Smad7. This study revealed that miR-93 is an important negative regulator in colon cancer and suggested that miR-93 may serve as a novel therapeutic agent that offers benefits for colon cancer treatment.

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Funding

The work was supported by the National Natural Science Foundation of China (81272706).

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None.

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Authors and Affiliations

  1. Department of Colorectal Cancer Surgery, Cancer Center, the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China

    Qingchao Tang, Qian Zhang, Rui Huang, Xu Guan & Xishan Wang

  2. Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, 150081, China

    Zhaoxia Zou, Chendan Zou, Zhongjing Han, Dayong Wang, Huiyan Wei & Xu Gao

  3. Department of General Surgery, the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China

    Qiang Li

  4. Colorectal Cancer Institute, the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China

    Xishan Wang

Authors
  1. Qingchao Tang
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  2. Zhaoxia Zou
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  4. Qian Zhang
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Correspondence to Xu Gao or Xishan Wang.

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Tang, Q., Zou, Z., Zou, C. et al. MicroRNA-93 suppress colorectal cancer development via Wnt/β-catenin pathway downregulating. Tumor Biol. 36, 1701–1710 (2015). https://doi.org/10.1007/s13277-014-2771-6

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  • DOI: https://doi.org/10.1007/s13277-014-2771-6

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