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RAB34 was a progression- and prognosis-associated biomarker in gliomas

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Tumor Biology

Abstract

The objective of this study is to explore the expression pattern, prognostic value, and functional role of RAB34 in gliomas. RAB34 messenger RNA (mRNA) expression was evaluated from low grade to high grade in 220 glioma patients of the Chinese Glioma Genome Atlas (CGGA). We therefore analyzed RAB34 mRNA expression in two validated datasets. For detecting the protein expression level of RAB34, another 104 glioma tissues were stained by immunohistochemistry. Gene ontology (GO) analysis and gene set variation analysis (GSVA) were used for functional annotation of RAB34. The mRNA and protein expression levels of RAB34 were both related to glioma grade progression and were inversely correlated with overall survival (OS) in high-grade glioma patients. GO analysis and GSVA showed that RAB34 sets related to migration were significantly enriched in the cases with RAB34 high expression. Pearson correlation analysis identified that genes including MMP-11, HSPB1, IGFBP2, HSPA6, IGFBP5, and MMP19 were positively correlated with RAB34. The expression of RAB34 is related to glioma grade progression and confers a poor prognosis in high-grade glioma patients.

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Acknowledgments

This research was supported by grants from the National High Technology Research and Development Program (No. 2012AA02A508), International Science and Technology Cooperation Program (No. 2012DFA30470), National 973 Program (No. 2011CB707804), and National Natural Science Foundation of China (No. 81201993).

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Authors and Affiliations

  1. Department of Neurosurgery, The Second Affiliated Hospital of Harbin Medical University, No. 246, Xuefu Road, Harbin, 150086, Heilongjiang Province, China

    Hong-jun Wang, Yong-li Li & Chuan-lu Jiang

  2. Department of Critical Care Medicine, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, Heilongjiang, China

    Yang Gao

  3. Department of Neurosurgery, Xuanwu Hospital of Capital Medical University, Beijing, 100053, People’s Republic of China

    Ling Chen

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  1. Hong-jun Wang
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  2. Yang Gao
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  3. Ling Chen
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  4. Yong-li Li
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  5. Chuan-lu Jiang
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Corresponding author

Correspondence to Chuan-lu Jiang.

Additional information

Hong-jun Wang and Yang Gao contributed equally.

Hong-jun Wang and Chuan-lu Jiang are members of the Chinese Glioma Cooperative Group (CGCG).

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Wang, Hj., Gao, Y., Chen, L. et al. RAB34 was a progression- and prognosis-associated biomarker in gliomas. Tumor Biol. 36, 1573–1578 (2015). https://doi.org/10.1007/s13277-014-2732-0

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  • DOI: https://doi.org/10.1007/s13277-014-2732-0

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