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Clinical significance of the low expression of FER1L4 in gastric cancer patients

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Tumor Biology

Abstract

Long non-coding RNA (lncRNA) is a new class of regulative non-coding RNA, with a length larger than 200 nucleotides. Recent studies found that there are close relations between disregulative lncRNAs and human tumors. However, the clinical significances are largely unknown. In this study, we investigated the lncRNA–Fer-1-like protein 4 (FER1L4) level in gastric cancer tissues and plasma. The FER1L4 level in human tissues and plasma were measured by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Then, the correlations between the tissue or plasma FER1L4 levels and clinicopathological factors were assessed. A receiver operating characteristic (ROC) curve was constructed for differentiating GC patients from controls. Compared to matched adjacent non-tumorous tissues, FER1L4 expression levels in 91.80 % (56/61) of gastric cancer tissues are significantly decreased. The low FER1L4 level were associated with tumor size (p < 0.001), histologic grade (p = 0.001), general classification (p < 0.001), depth of invasion (p < 0.001), lymphatic metastasis (p < 0.001), distant metastasis (p = 0.003), TNM stage (p < 0.001), vessel or nerve invasion (p < 0.001 or p = 0.003), and serum CA72-4 (p < 0.001). The area under the ROC curve (AUC) was up to 0.778 (p < 0.001) and the sensitivity and specificity were 67.2 and 80.3 %, respectively. Also, plasma FER1L4 was detected by qRT-PCR. Our data show that there was no difference of plasma FER1L4 level between healthy person and preoperative gastric cancer patients, with a sharp decline in 63.9 % (53/83) of gastric cancer patients 2 weeks after surgery (p = 0.028). Taken together, FER1L4 might play a crucial role in human gastric cancer and may be a new potential biomarker for clinical prognosis evaluation.

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References

  1. Wapinski O, Chang HY. Long noncoding RNAs and human disease. Trends Cell Biol. 2011;21:354–61.

    Article  CAS  PubMed  Google Scholar 

  2. Okazaki Y, Furuno M, Kasukawa T, Adachi J, Bono H, Kondo S, et al. Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs. Nature. 2002;420:563–73.

    Article  PubMed  Google Scholar 

  3. Kapranov P, Cheng J, Dike S, Nix DA, Duttagupta R, Willingham AT, et al. RNA maps reveal new RNA classes and a possible function for pervasive transcription. Science. 2007;316:1484–8.

    Article  CAS  PubMed  Google Scholar 

  4. Mercer TR, Dinger ME, Mattick JS. Long non-coding RNAs: insights into functions. Nat Rev Genet. 2009;10:155–9.

    Article  CAS  PubMed  Google Scholar 

  5. Li J, Xuan Z, Liu C. Long non-coding RNAs and complex human diseases. Int J Mol Sci. 2013;14:18790–808.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Clark MB, Mattick JS. Long noncoding RNAs in cell biology. Semin Cell Dev Biol. 2011;22:366–76.

    Article  CAS  PubMed  Google Scholar 

  7. Hauptman N, Glavac D. Long non-coding RNA in cancer. Int J Mol Sci. 2013;14:4655–69.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Matouk IJ, DeGroot N, Mezan S, Ayesh S, Abu-lail R, Hochberg A, et al. The H19 non-coding RNA is essential for human tumor growth. PLoS One. 2007;2:e845.

    Article  PubMed Central  PubMed  Google Scholar 

  9. Zhou Y, Zhang X, Klibanski A. MEG3 noncoding RNA: a tumor suppressor. J Mol Endocrinol. 2012;48:R45–53.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Wu HH, Lin WC, Tsai KW. Advances in molecular biomarkers for gastric cancer: miRNAs as emerging novel cancer markers. Expert Rev Mol Med. 2014. doi:10.1017/erm.2013.16.

    PubMed Central  PubMed  Google Scholar 

  11. Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM, et al. Treatment of gastric cancer. World J Gastroenterol. 2014;20:1635–49.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Duraes C, Almeida GM, Seruca R, Oliveira C, Carneiro F. Biomarkers for gastric cancer: prognostic, predictive or targets of therapy? Virchows Arch. 2014;464:367–78.

    Article  CAS  PubMed  Google Scholar 

  13. Song H, Sun W, Ye G, Ding X, Liu Z, Zhang S, et al. Long non-coding RNA expression profile in human gastric cancer and its clinical significances. J Transl Med. 2013;11:225.

    Article  PubMed Central  PubMed  Google Scholar 

  14. Li CH, Chen Y. Targeting long non-coding RNAs in cancers: progress and prospects. Int J Biochem Cell Biol. 2013;45:1895–910.

    Article  CAS  PubMed  Google Scholar 

  15. Tang JY, Lee JC, Chang YT, Hou MF, Huang HW, Liaw CC, et al. Long noncoding RNAs-related diseases, cancers, and drugs. Sci World J. 2013. doi:10.1155/2013/943539.

    Google Scholar 

  16. Sun W, Wu Y, Yu X, Liu Y, Song H, Xia T, et al. Decreased expression of long noncoding RNA AC096655.1-002 in gastric cancer and its clinical significance. Tumour Biol. 2013;34:2697–701.

    Article  CAS  PubMed  Google Scholar 

  17. Qi P, Xu MD, Ni SJ, Huang D, Wei P, Tan C, et al. Low expression of LOC285194 is associated with poor prognosis in colorectal cancer. J Transl Med. 2013;11:122.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. He X, Bao W, Li X, Chen Z, Che Q, Wang H, et al. The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis. Int J Mol Med. 2014;33:325–32.

    CAS  PubMed  Google Scholar 

  19. Weber DG, Johnen G, Casjens S, Bryk O, Pesch B, Jöckel KH, et al. Evaluation of long noncoding RNA MALAT1 as a candidate blood-based biomarker for the diagnosis of non-small cell lung cancer. BMC Res Notes. 2013;6:518.

    Article  PubMed Central  PubMed  Google Scholar 

  20. Wang W, Li YF, Sun XW, Chen YB, Li W, Xu DZ, et al. Prognosis of 980 patients with gastric cancer after surgical resection. Chin J Cancer. 2010;29:923–30.

    Article  CAS  PubMed  Google Scholar 

  21. Selcukbiricik F, Tural D, Buyukunal E, Serdengeçti S. Perineural invasion independent prognostic factors in patients with gastric cancer undergoing curative resection. Asian Pac J Cancer Prev. 2012;13:3149–52.

    Article  PubMed  Google Scholar 

  22. Saito H, Osaki T, Murakami D, Sakamoto T, Kanaji S, Oro S, et al. Macroscopic tumor size as a simple prognostic indicator in patients with gastric cancer. AmJ Surg. 2006;192:296–300.

    Article  Google Scholar 

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Acknowledgements

This work was supported by National Natural Science Foundation of China (no. 81171660), the Zhejiang Provincial Natural Science Foundation of China (no. Y14C060003), the Natural Science Foundation of Ningbo (no. 2012A610207), the Ningbo Social Development Research Project (no. 2012C50005), the Scientific Innovation Team Project of Ningbo (no. 2011B82014), the Project of Key Disciplines in Ningbo (nos. XKL11D2127 and XKL11D2128), the Scientific Research Foundation of Graduate School of Ningbo University (no. G14069), and the K. C. Wong Magna Fund in Ningbo University.

Conflicts of interest

None.

Expression microarray data

Expression microarray data accessible at NCBI GEO database, accession GSE478 (Guo, 2013)

Ethics approval

This study was conducted with the approval of The Human Research Ethics Committee of Ningbo University.

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Not commissioned; externally peer reviewed.

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Authors and Affiliations

  1. Department of General Surgery, The Affiliated Hospital of Ningbo University School of Medicine, Ningbo, 315020, China

    Zhong Liu, Lin Tan & Huajun Shi

  2. Department of Biochemistry and Molecular Biology, and Zhejiang Provincial Key Laboratory of Pathophysiology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo, 315211, China

    Yongfu Shao, Shengcan Chen & Junming Guo

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  1. Zhong Liu
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  2. Yongfu Shao
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  3. Lin Tan
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  4. Huajun Shi
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  6. Junming Guo
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Correspondence to Yongfu Shao or Junming Guo.

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Liu, Z., Shao, Y., Tan, L. et al. Clinical significance of the low expression of FER1L4 in gastric cancer patients. Tumor Biol. 35, 9613–9617 (2014). https://doi.org/10.1007/s13277-014-2259-4

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  • DOI: https://doi.org/10.1007/s13277-014-2259-4

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