Abstract
In this study, 12 dogs affected by canine transmissible venereal tumor (CTVT) and testicular seminoma tumor were studied retrospectively. The cytological sample was smeared onto a glass slide and either air-dried for May-Grünwald-stain, and masses were surgically removed. The tumors were grossly examined, and sections of 4-μm thick were obtained from each sample and stained with H&E. For chemotherapy, vincristine sulfate was administered weekly as an infusion over 3 min via the cephalic vein at a dose of 0.025 mg/kg after diluting with physiological saline to a total amount of 10 ml. If no remission was observed after 8 weeks, chemotherapy was continued with weekly doxorubicin infusion at a dose of 1 mg/kg. All the tumor samples were divided into four cytohistopathologic groups, namely: multilobular (six cases), papillary (two cases), pedunculated (two cases), and tubular (two cases of seminoma). The most frequently represented tumor type was multilobular (6/10, 60 %) followed by pedunculated (2/10, 20 %), papillary (2/10, 20 %), and tubular (two cases of seminoma, 100 %). Cytological smears from eight tumors in regression after chemotherapy were poorly cellular, and many cells were fragmented. In two progressive tumors, there was an average of 1,406 ± 972 CTVT 200 cells/μl or 96 · 71 % of total cells counted. Thus, tumor cells represented 96 · 71 % of total cells within the biopsy specimens and the leukocytes 4 · 29 % (leukocyte, tumor cell ratio = 0 · 062 ± 0 · 031). In eight regressive tumors, there was an average of 1,245 ± 1,032 CTVT 200 cells/μl or 97 · 31 % of total cells counted. Thus, tumor cells represented 97 · 31 % of total cells and leukocytes 2 · 69 % (leukocyte, tumor cell ratio = 0 · 071 ± 0 · 174). Our data suggested that combination treatment with vincristine and doxorubicin in the future could be an excellent therapeutic alternative for the treatment of TVT for probably reducing the resistance to vincristine, and also, treatment success could easily be followed by the cytological changes.
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The authors thank Dr. Saeid Fathi and Dr. Mehdi Aghamohammad Hassan, Faculty of Veterinary Medicine, Tehran University, Iran, for their help with this manuscript.
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This article has been retracted at the request of the Editor-in-Chief, the International Society of Oncology and BioMarkers (ISOBM) and the Publisher per the Committee on Publication Ethics guidelines. The article shows evidence of irregularities in authorship during the submission process, irregularities in the methods section, there is strong reason to believe that the peer review process was compromised and the authors have plagiarized substantial parts from the following article:
D. Nak, Y. Nak, I. T. Cangul, B. Tuna, A Clinico-pathological Study on the Effect of Vincristine on Transmissible Venereal Tumour in Dogs, Journal of Veterinary Medicine Series A. 2005; 52:7 366-370
DOI: 10.1111/j.1439-0442.2005.00743.x
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An erratum to this article is available at http://dx.doi.org/10.1007/s13277-016-5473-4.
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Javanbakht, J., Pedram, B., Taheriyan, M.R. et al. RETRACTED ARTICLE: Canine transmissible venereal tumor and seminoma: a cytohistopathology and chemotherapy study of tumors in the growth phase and during regression after chemotherapy. Tumor Biol. 35, 5493–5500 (2014). https://doi.org/10.1007/s13277-014-1723-5
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DOI: https://doi.org/10.1007/s13277-014-1723-5