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Expression of the molecular chaperone αB-crystallin in infiltrating ductal breast carcinomas and the significance thereof: an immunohistochemical and proteomics-based strategy

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Tumor Biology

Abstract

This study aims to evaluate αB-crystallin expression in infiltrating ductal breast carcinomas (IDCAs), as well as, its prognostic significance. Using a two-dimensional electrophoresis matrix-assisted laser desorption/ionisation-time of flight mass spectrometry investigation coupled to an immunohistochemical approach, we have assessed the expression of αB-crystallin in IDCAs, as well as, in other types of breast tumors (invasive lobular carcinomas, medullary carcinomas, and in situ ductal carcinomas). Correlation between αB-crystallin expression and clinicopathological parameters of breast cancer has also been investigated. Proteomic analyses revealed an increased expression of αB-crystallin in IDCA tumors compared to adjacent nontumor tissues. Overexpression of this molecular chaperone was further confirmed in 51 tumor specimens. Statistical analyses revealed, however, no significant correlations between αB-crystallin expression and clinicopathological parameters of the disease (tumor stage, patient age, hormone receptors, SBR grade, and lymph node metastases). This study demonstrates the upregulation of αB-crystallin in IDCA tissues which may highlight its possible involvement in breast cancer development. Our findings do not, however, support the involvement of this molecular chaperone in the progression of this disease.

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Acknowledgments

This work was supported by le Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, le Ministère de la Santé Publique de la République Tunisienne and by the Centre National de Recherche Scientifique (Strasbourg, France).

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Authors and Affiliations

  1. Laboratoire d’Immuno-Oncologie Moléculaire, Faculté de Médecine de Monastir, Monastir, Tunisia

    Maria Kabbage, Lotfi Chouchane & Karim Chahed

  2. Département de Pathologie, Centre Hospitalo-Universitaire-Farhat-Hached, Sousse, Tunisia

    Mounir Trimeche

  3. Institut Supérieur des Sciences Appliquées et Technologiques ISSAT, Sousse, Tunisia

    Hela ben Nasr

  4. Plate Forme Protéomique, Institut de Biologie Moléculaire et Cellulaire, CNRS, 67084, Strasbourg, France

    Philippe Hammann & Lauriane Kuhn

  5. Ecole préparatoire des ingénieurs de Sfax (IPEIS), Sfax, Tunisia

    Bechr Hamrita

  6. Service d’Obstétrique et des maladies féminines, Centre Hospitalo-Universitaire-Farhat-Hached, Sousse, Tunisia

    Anouar Chaieb

  7. Faculté des Sciences de Sfax, Université de Sfax, Sfax, Tunisia

    Karim Chahed

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  1. Maria Kabbage
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  2. Mounir Trimeche
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  8. Lotfi Chouchane
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  9. Karim Chahed
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Correspondence to Karim Chahed.

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Kabbage, M., Trimeche, M., ben Nasr, H. et al. Expression of the molecular chaperone αB-crystallin in infiltrating ductal breast carcinomas and the significance thereof: an immunohistochemical and proteomics-based strategy. Tumor Biol. 33, 2279–2288 (2012). https://doi.org/10.1007/s13277-012-0490-4

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  • DOI: https://doi.org/10.1007/s13277-012-0490-4

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