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Anti-inflammatory effect of Chunkoongkeigi-tang on IL-1β-induced inflamed A549 by the inhibition of COX-2 expression

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Abstract

Backgrounds

Inflammatory lung disease can arise due to the immoderate expression of pro-inflammatory genes and the disharmony of complex cytokines. Chunkoongkeigi-tang (CKT) has been prescribed to patients with asthmatic symptoms in herbal medicine clinics. However, the effects of CKT on the inflamed human lung cell line A549 have yet to be revealed. Thus we investigate whether CKT can suppress inflammatory response on interleukin 1β (IL-1β)-induced inflamed A549.

Methods

In an effort to understand the inhibitory activity of CKT on inflamed A549, we use IL-1β to induce inflammatory gene COX-2 expressions on A549. Inhibition of COX-2 expression and inflammatory cytokine secretion through blocking mitogen activated protein kinase (MAPK) pathway and transcriptional nuclear factor kappa B (NF-κB) activity was confimed by western blot and real-time PCR. Compounds in CKT were identified by liquid chromatography-mass spectrometry (LC-MS).

Results

CKT inhibited increased COX-2 in an IL-1β-induced inflammatory state of A549. We confirmed that CKT regulated the mitogen activated protein kinase (MAPK) pathway and transcriptional nuclear factor kappa B (NF-κB). Additionally, a suppressive effect of CKT on the mRNA expression levels of cytokine-related asthma ((IL-5, IL-6, IL-17 and TNF-α) was noted in IL-1β-induced inflamed A549. According to LC-MS analysis, we identified several components in CKT which can be expected to be the active compounds of the anti-inflammatory effect of CKT.

Conclusion

We verify the anti-inflammatory effect of CKT via the reduction of the IL-1β-induced increased COX-2 expression through the MAPK and NF-κB pathways, suggesting useful anti-asthmatic potential by inhibiting inflammatory cytokines, especially in patients with asthma.

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Authors and Affiliations

  1. College of Korean Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Eun-Jin An & Hyeung-Jin Jang

  2. Department of Science in Korean Medicine, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Eun-Jin An & Hyeung-Jin Jang

  3. Division of Allergy, Immune and Respiratory System, Department of Internal Medicine, College of Korean Medicine, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea

    Kwanil Kim

  4. Department of Microbiology, Catholic Kwandong University, College of Medicine, Gangneung, 25601, Republic of Korea

    Daeho Kwon

Authors
  1. Eun-Jin An
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  2. Kwanil Kim
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  4. Hyeung-Jin Jang
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Correspondence to Kwanil Kim, Daeho Kwon or Hyeung-Jin Jang.

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An, EJ., Kim, K., Kwon, D. et al. Anti-inflammatory effect of Chunkoongkeigi-tang on IL-1β-induced inflamed A549 by the inhibition of COX-2 expression. Mol. Cell. Toxicol. 15, 93–102 (2019). https://doi.org/10.1007/s13273-019-0011-2

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  • DOI: https://doi.org/10.1007/s13273-019-0011-2

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