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Statistical analysis and genetic diversity of three dog breeds using simple sequence repeats

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Abstract

There are more than 400 pure dog breeds developed through intentional artificial selection and purebred breeding. Purebred animals have higher risk of inbreeding depression and hereditary diseases. We investigated the genetic diversity and structure of three dog breeds in South Korea by using 12 microsatellite loci for one Korean native dog breed, Sapsaree, and two foreign breeds, German shepherd and Belgian Malinois. The mean allele number of nine loci across all dog breeds was 4.833, and the number of alleles per locus ranged from 2 to 8. The mean of expected and observed heterozygosity were 0.415 and 0.577, respectively. Sapsaree, Korean native dog, had higher level of genetic diversity than the foreign German shepherd and Belgian Malinois. The highest mean value of polymorphism information content was found in Sapsaree (0.480), followed by Belgian Malinois (0.373) and German shepherd (0.355). Pairwise genetic differentiation was estimated using fixation index F ST. Sapsaree and German shepherd (F ST = 0.2536) and Sapsaree and Belgian Malinois (F ST = 0.2522) had very great genetic differentiation, while moderate level of genetic differentiation was observed between German shepherd and Belgian Malinois (F ST = 0.1003). These genetic information and structure of the three dog breeds will be effective in conservation and preservation of the genetic diversity of the three dog breeds.

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Acknowledgments

This research was supported by the funding provided by the AGENDA project (Project No. PJ009254) from the National Institute of Animal Science, Rural Development Administration (RDA).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 609-735, Republic of Korea

    Hoim Jeong, Jungwoo Eo, Yun-Jeong Kwon, Hee-Eun Lee, Yuri Choi, Jeong-An Gim & Heui-Soo Kim

  2. Division of Animal Genomics and Bioinformatics, National Institute of Animal Science, Rural Development Administration, Suwon, 441-706, Republic of Korea

    Bong-Hwan Choi, Tae-Hun Kim & Hwan-Hoo Seong

  3. Department of Equine Sciences, Sorabol College, Gyeongju, Gyeongbuk, 780-711, Republic of Korea

    Dong-Hoon Lee

  4. School of Life Science and Biotechnology, Kyungpook National University, Taegu, 702-701, Republic of Korea

    Ji-Hong Ha

  5. Sapsaree Breeding Research Institute, Gyeongsan, Gyeongbuk, 712-844, Republic of Korea

    Ji-Hong Ha & Kook-Il Han

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  1. Hoim Jeong
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  2. Bong-Hwan Choi
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  3. Jungwoo Eo
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  4. Yun-Jeong Kwon
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  8. Tae-Hun Kim
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  9. Hwan-Hoo Seong
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  10. Dong-Hoon Lee
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  11. Ji-Hong Ha
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  12. Kook-Il Han
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  13. Heui-Soo Kim
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Correspondence to Heui-Soo Kim.

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Jeong, H., Choi, BH., Eo, J. et al. Statistical analysis and genetic diversity of three dog breeds using simple sequence repeats. Genes Genom 36, 883–889 (2014). https://doi.org/10.1007/s13258-014-0237-2

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